LOCUS       sequence-397592-       10195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses LOV-Turbo1-G3BP1 in the mammalian cytosol under dox 
            inducible promoter, lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Qin W, Cheah JS, Xu C, Messing J, Freibaum BD, Boeynaems S, Taylor 
            JP, Udeshi ND, Carr SA, Ting AY
  TITLE     Dynamic mapping of proteome trafficking within and between living 
            cells by TransitID.
  JOURNAL   Cell. 2023 Jun 23:S0092-8674(23)00596-2. doi: 
            10.1016/j.cell.2023.05.044.
  PUBMED    37385249
REFERENCE   2  (bases 1 to 10195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2023 
            Jun 23:S0092-8674(23)00596-2. doi: 10.1016/j.cell.2023.05.044."
COMMENT     Sequence Label: V5-LOV-Turbo1-G3BP1_pTRE-G418
FEATURES             Location/Qualifiers
     source          1..10195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2805..2922
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2976..3290
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2984..3002
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3020..3038
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3055..3073
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3091..3109
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3127..3145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3162..3180
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3198..3216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3263..3287
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3307..3316
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3316..3357
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        6180..6690
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6609..6627
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(6700..7317,7318..7323,7324..7359,7360..7362,7363..7398,
                     7399..7401,7402..7437,7438..7443)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             7453..7509
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7510..8301
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7510..8301
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7561..7580)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8171..8190
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             8492..8725
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8797..8918
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8834..8853)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8888..8907
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8958..9093
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9093..9164
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9165..9233,9234..10025)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacaattct cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2821 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2881 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2941 agcagagatc cactttggcc gcgaatcgat atgtcgagtt tactccctat cagtgataga
     3001 gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt ttactcccta
     3061 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     3121 gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca gtgatagaga
     3181 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac
     3241 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagaattg
     3301 gctagcgcca ccatgggcaa gcccatcccc aaccccctgc tgggcctgga cagcaccggt
     3361 accaaagaca atactgtgcc tctgaagctg atcgctctcc tggctaatgg cgagttccat
     3421 agtggcgaac agctgggaga aaccctgggc atgtccaggg ccgctatcaa caagcacatt
     3481 cagactctgc gcgactgggg cgtggacgtg ttcaccgtgc ccggaaaggg ctactctctg
     3541 cccgagccta tcccgctgct gaacgctaaa cagattctgg gacagctgga gtttcgggca
     3601 accacactgg aacggatcga gaaaagcttc gtgattactg atccgagact gcctgacaac
     3661 ccaatcattt ttgtgagcga ttccttcctg cagctgacag aatattctcg ggaagagatc
     3721 ctggggcgca attgccgttt tctgcaggga cccgagacag accgtgccac tgttcggaaa
     3781 atcagagatg ctattgacaa ccagactgaa gtgaccgttc agctgatcaa ttataccaag
     3841 agcggcaaga agttctggaa cgtgttccac ctgcagccga tgcgcgatta taagggcgac
     3901 gtccagtact tcattggcgt gcagctggat ggcaccgaac gtcttcatgg cgccgctgag
     3961 cgtgaggcgg tctgcctggt caaaaagaca gcctttcaga ttgctgtggc agtcctgcct
     4021 gtggtcgact ccaccaatca gtacctgctg gatcgaatcg gcgagctgaa gagtggggat
     4081 gcttgcattg cagaatatca gcaggcaggg agaggaagca gagggaggaa atggttctct
     4141 ccttttggag ctaacctgta cctgagtatg ttttggcgcc tgaagcgggg accagcagca
     4201 atcggcctgg gcccggtcat cggaattgtc atggcagaag cgctgcgaaa gctgggagca
     4261 gacaaggtgc gagtcaaatg gcccaatgac ctgtatctgc aggatagaaa gctggcaggc
     4321 atcctggtgg agctggccgg aataacaggc gatgctgcac agatcgtcat tggcgccggg
     4381 attaacgtgg ctatgaggcg cgtggaggaa agcgtggtca atcagggctg gatcacactg
     4441 caggaagcag ggattaacct ggacaggaat actctggccg ctacgctgat ccgagagctg
     4501 cgggcagccc tggaactgtt cgagcaggaa ggcctggctc catatctgcc acggtgggag
     4561 aagctggata acttcatcaa tagacccgtg aagctgatca ttggggacaa agagattttc
     4621 gggattagcc gggggattga taaacaggga gccctgctgc tggaacagga cggagttatc
     4681 aaaccctgga tgggcggaga aatcagtctg cggtctgccg aaaagggatc cggtggaggc
     4741 gggtctggag gcgggggtag tatggtgatg gagaagccca gccctctact agtaggccgg
     4801 gaatttgtga gacagtatta cacactgctg aaccaggccc cagacatgct gcatagattt
     4861 tatggaaaga actcttctta tgtccatggg ggattggatt caaatggaaa gccagcagat
     4921 gcagtctacg gacagaaaga aatccacagg aaagtgatgt cacaaaactt caccaactgc
     4981 cacaccaaga ttcgccatgt tgatgctcat gccacgctaa atgatggtgt ggtagtccag
     5041 gtgatggggc ttctctctaa caacaaccag gctttgagga gattcatgca aacgtttgtc
     5101 cttgctcctg aggggtctgt tgcaaataaa ttctatgttc acaatgatat cttcagatac
     5161 caagatgagg tctttggtgg gtttgtcact gagcctcagg aggagtctga agaagaagta
     5221 gaggaacctg aagaaagaca gcaaacacct gaggtggtac ctgatgattc tggaactttc
     5281 tatgatcagg cagttgtcag taatgacatg gaagaacatt tagaggagcc tgttgctgaa
     5341 ccagagcctg atcctgaacc agaaccagaa caagaacctg tatctgaaat ccaagaggaa
     5401 aagcctgagc cagtattaga agaaactgcc cctgaggatg ctcagaagag ttcttctcca
     5461 gcacctgcag acatagctca gacagtacag gaagacttga ggacattttc ttgggcatct
     5521 gtgaccagta agaatcttcc acccagtgga gctgttccag ttactgggat accacctcat
     5581 gttgttaaag taccagcttc acagccccgt ccagagtcta agcctgaatc tcagattcca
     5641 ccacaaagac ctcagcggga tcaaagagtg cgagaacaac gaataaatat tcctccccaa
     5701 aggggaccca gaccaatccg tgaggctggt gagcaaggtg acattgaacc ccgaagaatg
     5761 gtgagacacc ctgacagtca ccaactcttc attggcaacc tgcctcatga agtggacaaa
     5821 tcagagctta aagatttctt tcaaagttat ggaaacgtgg tggagttgcg cattaacagt
     5881 ggtgggaaat tacccaattt tggttttgtt gtgtttgatg attctgagcc tgttcagaaa
     5941 gtccttagca acagacccat catgttcaga ggtgaggtcc gtctgaatgt cgaagagaag
     6001 aagactcgag ctgccaggga aggcgaccga cgagataatc gccttcgggg acctggaggc
     6061 cctcgaggtg ggctgggtgg tggaatgaga ggccctcccc gtggaggcat ggtgcagaaa
     6121 ccaggatttg gagtgggaag ggggcttgcg ccacggcagt aatagggccg aattcatccg
     6181 gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct
     6241 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc
     6301 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct
     6361 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg
     6421 gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg
     6481 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc
     6541 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg
     6601 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc
     6661 gttgaccgaa tcaccgacct ctctccccag caattcacca tgtctagact ggacaagagc
     6721 aaagtcataa acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca
     6781 aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac
     6841 aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc
     6901 tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc
     6961 tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag
     7021 aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg
     7081 gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag
     7141 gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc
     7201 ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt
     7261 ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg
     7321 ccgaccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac
     7381 tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc
     7441 gggggaagcg gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac
     7501 cctggaccta ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     7561 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     7621 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     7681 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     7741 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     7801 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     7861 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     7921 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     7981 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
     8041 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     8101 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     8161 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     8221 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     8281 cttcttgacg agttcttctg ataaccgcgg cctgctgccg gctctgcggc ctcttccgcg
     8341 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgtttc
     8401 gcctcggcgt ccggactaga ggtaccttta agaccaatga cttacaaggc agctgtagat
     8461 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     8521 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     8581 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8641 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8701 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     8761 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     8821 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8881 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     8941 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     9001 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     9061 tccagaagta gtgaggaggc ttttttggag gcctttcaaa tatgtatccg ctcatgagac
     9121 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     9181 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     9241 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     9301 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     9361 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     9421 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     9481 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     9541 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     9601 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     9661 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     9721 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     9781 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     9841 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     9901 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     9961 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    10021 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    10081 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    10141 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
