LOCUS       sequence-397596-        4663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gfp BioPart for AWC neurons. (1) Mark neurons or (2) cell-specific 
            expression of transgenes (N or C-terminal fusions, or untagged). 
            Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH90 - [AWCp | gfp | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1890..1906)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1890..1906)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1903..1925)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2078..2666)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2158..2177)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2783..2807)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2907..3698,3699..3767))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3530..3549
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3768..3872)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3971..3993
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3985..4002
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3986..4002
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4626..4659)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tcaaaaatga ttacgccaag ctgtaagttt aaacatgatc ttactaacta actattctca
       61 tttaaatttt cagagcttaa aaatggctga aatcactcac aacgatggat acgctaatgc
      121 caaccgagat gtaccttttt tcaaagtgca gaaagtgaat ctaattaatt tgaagccaaa
      181 ctttgatgca tcaaaaaaac tgccaacaac tgctgatctt acgatggact ccaagaaaaa
      241 cgtgacaatg tgtcgaaatc tttgaaatct cgtttttgag tgagctcttt caaacaaaag
      301 cgaaagcttc tactcattca attttaaaag tttgaataat attaatagta ttacgcaata
      361 atcccggatt agactcacta taaaaagtag tccaaagtta gcagtagcac atcaagaaaa
      421 aagagaccaa aaatggggcc ctctaagggc gaagaattgt tcacgggagt agtcccaatc
      481 ttagtcgagc ttgacggagg tgagttttat tatcgatttt cgggattttc tacttgtaat
      541 taccttttgt ttttaataag ttgctttttt ccagatgtta atggccacaa gttttccgtg
      601 tctggagaag gagaaggcga cgctacctac ggtaagctga ccctgaagtt catttgcact
      661 actggcaagt taccagtacc atggccgacc ctggtgacaa cattttgcta cggggttcaa
      721 tgcttctcca gatacccagg taggttttca gtttcaattt ttgttgcaac tacctcaact
      781 ctatgttttc agatcatatg aaaagacacg acttttttaa atccgccatg ccagagggtt
      841 acgttcaaga acgtaccatt ttttttaagg acgatggtaa ctacaagaca cgtgcggagg
      901 ttaaatttga aggcgatact ctcgtgaacc gtattgagct caaggtaaat ttttcaagct
      961 tctaaattcc aaaataaacc attttaattt cttaattcca gggaatcgat ttcaaggaag
     1021 atggaaacat tttgggccac aagcttgaat ataattataa tagtcataat gtctacatta
     1081 tggccgacaa acaaaaaaac ggtattaagg ttaacttcaa gattcgccac aatatcgaag
     1141 atggatctgt ccaacttgct gaccactacc aacaaaatac tccaattgga gatggaccgg
     1201 ttttgcttcc agataaccac tacctttcca ctcagtctgc cctctctaag gatccgaatg
     1261 aaaaacgtga ccatatggtt cttttggagt tcgttaccgc agcaggaatt actcacggaa
     1321 tggatgaact gtacaagccc gggtgatggt ctctgagcta tgcaaaatcc tttcaagcat
     1381 tcccttcttc tctatcactc ttctttcttt ttgtcaaaaa attctctcgc taatttattt
     1441 gcttttttaa tgttattatt ttatgacttt ttatagtcac tgaaaagttt gcatctgagt
     1501 gaagtgaatg ctatcaaaat gtgattctga tatccaggaa acggagaatc tgtgcggcaa
     1561 tgtcttatca tttgaaacgc gccgtatcga tgctgacatc atgcggctcc cgtgttagtc
     1621 gagaagatgg gtacgggcct ttattcatcc aacactctac ggcttcttca agagctagta
     1681 gggcaccctg tagttggaaa gggattattt cgtatggcga gcccataccg tctttcttgc
     1741 ggaagcttaa cacgatagga agttggatag tttcgaacga tggttccata cggaacgctg
     1801 tctcaaggat gagtctgacg gagtgtaact cgaaggctag gtggaggctc agtgatgata
     1861 agtctgcgat ggtggatgca tgtgtcatgg tcatagctgt ttcctgtgtg aaattgttat
     1921 ccgctcagag ggcacaatcc tattccgcgc tatccgacaa tctccaagac attaggtgga
     1981 gttcagttcg gcgtatggca tatgtcgctg gaaagaacat gtgagcaaaa ggccagcaaa
     2041 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2101 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2161 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2221 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2281 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2341 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2401 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2461 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     2521 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2581 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2641 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2701 ctctattcaa caaagccgcc gtcccgtcaa gtcagcgtaa atgggtaggg ggcttcaaat
     2761 cgtcctcgtg ataccaattc ggagcctgct tttttgtaca aacttgttga taatggcaat
     2821 tcaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2881 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2941 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3001 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga gccacgctca
     3061 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3121 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3181 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3241 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3301 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3361 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3421 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3481 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3541 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3601 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3661 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3721 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3781 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3841 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccagatacc
     3901 tgaaacaaaa cccatcgtac ggccaaggaa gtctccaata actgtgatcc accacaagcg
     3961 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtcatgcata atccgcacgc
     4021 atctggaata aggaagtgcc attccgcctg acctaagtgc ttttcgagat cgccaaaccg
     4081 gagaacgatc aggagaatga tgagtcgcca caggagtcgg caagatacgt gcgttataga
     4141 tttgctccaa actttttgaa actcaaaacg gtcggagcaa ctgtggaatt caaagtagga
     4201 gacatcccaa tccatcattt ccgaatgatc gaacgtcact tcttcaaaga tcgccttctg
     4261 aagtgttttg actttgaatt cggattctgt attccgaatt cacgaaacaa ctgtgaacat
     4321 atctatgagt tccctcaact ctctcaacaa ctcagtgagt cattattcta aaaagtacaa
     4381 ttcaaaagac taatctcttt ttcagtggac gacatgatca acaatccaaa cgagactcgt
     4441 tctgacagct tctatttcgt cgataacaaa ctcgtcatgc acaacaaagc cgactactca
     4501 tatgatgcat aaatcttcat tgttgagttt atcttgttga tttttgaata aattatcaac
     4561 tctttacttt ttaatgggtt atgaaataaa taaacattga aaactgataa acaacgttca
     4621 tctctataac ttcgtatagc atacattata cgaagttatg ata
//
