LOCUS       sequence-397598-        4645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet BioPart for IL2LR neurons. (1) Mark neurons or (2) 
            cell-specific expression of transgenes (N or C-terminal fusions, or 
            untagged). Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4645)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH87 - [IL2LRp | wrmScarlet | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1889..1905)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1889..1905)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1902..1924)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2077..2665)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2157..2176)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2782..2806)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2906..3697,3698..3766))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3529..3548
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3767..3871)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3970..3992
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3984..4001
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3985..4001
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4625..4645,1..13))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tatacgaagt tatgatatca aaaatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaatgccag gagaaaatgg aattgctgac aattttaaga tggttgaaga
      181 aaatgcgatt actactgaag tacggctttt tgctgtttga attctcaagg tttttttgga
      241 ataaaaaaca tttttcggaa ctcctactaa tgaataattg cttctctact tgcaaaaaac
      301 aacattccca agactaaaga atgttggctc cctgtcctaa tggacacaca tctccttgga
      361 aatgctccat gggcaacgaa tagaatcgca aagcaacacg aaaactgaaa gaattcattt
      421 gtatttcttt tgctaaaaag agaccaaaaa tggggcccgt ttcaaaggga gaggccgtta
      481 taaaagaatt tatgcgtttc aaggtccaca tggagggatc catgaacgga cacgagttcg
      541 agatcgaggg agagggagag ggacgtccat acgagggaac ccaaaccgcc aaggtgagtt
      601 ttattatcga ttttcgggat tttctacttg taattacctt ttgtttttaa taagttgctt
      661 ttttccagct caaggtcacc aagggaggac cactcccatt ctcctgggac atcctctccc
      721 cacaattcat gtacggatcc cgtgccttca ccaagcaccc agccgacatc ccagactact
      781 acaagcaatc cttcccagag ggattcaagt gggagcgtgt catgaacttc gaggacggag
      841 gagccgtcac cgtcacccaa ggtaggtttt cagtttcaat ttttgttgca actacctcaa
      901 ctctatgttt tcagacacct ccctcgagga cggaaccctc atctacaagg tcaagctccg
      961 tggaaccaac ttcccaccag acggaccagt catgcaaaag aagaccatgg gatgggaggc
     1021 ctccaccgag cgactctacc cagaggacgg agtcctcaag ggagacatca aggtaaattt
     1081 ttcaagcttc taaattccaa aataaaccat tttaatttct taattccaga tggccctccg
     1141 gctcaaggac ggaggacgtt acctcgccga cttcaagacc acctacaagg ccaagaagcc
     1201 agtccaaatg ccaggagcct acaacgtcga ccgtaagctc gacatcacct cccacaacga
     1261 ggactacacc gtcgtcgagc aatacgagcg ttccgaggga cgtcactcca ccggaggaat
     1321 ggacgagctc tacaagcccg ggtgatggtc tctgagctat gcaaaatcct ttcaagcatt
     1381 cccttcttct ctatcactct tctttctttt tgtcaaaaaa ttctctcgct aatttatttg
     1441 cttttttaat gttattattt tatgactttt tatagtcact gaaaagtttg catctgagtg
     1501 aagtgaatgc tatcaaaatg tgattctgat atccaggaaa cggagaatct gtgcggcaat
     1561 gtcttatcat ttgaaacgcg ccgtatcgat gctgacatca tgcggctccc gtgttagtcg
     1621 agaagatggg tacgggcctt tattcatcca acactctacg gcttcttcaa gagctagtag
     1681 ggcaccctgt agttggaaag ggattatttc gtatggcgag cccataccgt ctttcttgcg
     1741 gaagcttaac acgataggaa gttggatagt ttcgaacgat ggttccatac ggaacgctgt
     1801 ctcaaggatg agtctgacgg agtgtaactc gaaggctagg tggaggctca gtgatgataa
     1861 gtctgcgatg gtggatgcat gtgtcatggt catagctgtt tcctgtgtga aattgttatc
     1921 cgctcagagg gcacaatcct attccgcgct atccgacaat ctccaagaca ttaggtggag
     1981 ttcagttcgg cgtatggcat atgtcgctgg aaagaacatg tgagcaaaag gccagcaaaa
     2041 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2101 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2161 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2221 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2281 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2341 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2401 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2461 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     2521 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2581 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2641 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2701 tctattcaac aaagccgccg tcccgtcaag tcagcgtaaa tgggtagggg gcttcaaatc
     2761 gtcctcgtga taccaattcg gagcctgctt ttttgtacaa acttgttgat aatggcaatt
     2821 caaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2881 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     2941 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3001 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagag ccacgctcac
     3061 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3121 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3181 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3241 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3301 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3361 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3421 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3481 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3541 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3601 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3661 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3721 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3781 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3841 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccagatacct
     3901 gaaacaaaac ccatcgtacg gccaaggaag tctccaataa ctgtgatcca ccacaagcgc
     3961 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tcatgcataa tccgcacgca
     4021 tctggaataa ggaagtgcca ttccgcctga cctaagtgct tttcgagatc gccaaaccgg
     4081 agaacgatca ggagaatgat gagtcgccac aggagtcggc aagatacgtg cgttatagat
     4141 ttgctccaaa ctttttgaaa ctcaaaacgg tcggagcaac tgtggaattc aaagtaggag
     4201 acatcccaat ccatcatttc cgaatgatcg aacgtcactt cttcaaagat cgccttctga
     4261 agtgttttga ctttgaattc ggattctgta ttccgaattc acgaaacaac tgtgaacata
     4321 tctatgagtt ccctcaactc tctcaacaac tcagtgagtc attattctaa aaagtacaat
     4381 tcaaaagact aatctctttt tcagtggacg acatgatcaa caatccaaac gagactcgtt
     4441 ctgacagctt ctatttcgtc gataacaaac tcgtcatgca caacaaagcc gactactcat
     4501 atgatgcata aatcttcatt gttgagttta tcttgttgat ttttgaataa attatcaact
     4561 ctttactttt taatgggtta tgaaataaat aaacattgaa aactgataaa caacgttcat
     4621 ctctataact tcgtatagca tacat
//
