LOCUS       sequence-397601-        4645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet BioPart for AWC neurons. (1) Mark neurons or (2) 
            cell-specific expression of transgenes (N or C-terminal fusions, or 
            untagged). Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4645)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH85 - [AWCp | wrmScarlet | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1883..1899)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1883..1899)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1896..1918)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2071..2659)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2151..2170)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2776..2800)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2900..3691,3692..3760))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3523..3542
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3761..3865)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3964..3986
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3978..3995
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3979..3995
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4619..4645,1..7))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aagttatgat atcaaaaatg attacgccaa gctgtaagtt taaacatgat cttactaact
       61 aactattctc atttaaattt tcagagctta aaaatggctg aaatcactca caacgatgga
      121 tacgctaatg ccactccaaa aaattgatgt ttcgcgatta tttcaaggca actatacaaa
      181 atggtaattt taataccttt ctcgatttaa tttcgtcata actttgcttc gtcataaaaa
      241 taagcttact catcccccat tctatacaaa cttattttca attgtttagc tcttattcaa
      301 ctaaatccta ttccccctat tctccttaac attccttaaa tatcaaactc taaaacaaat
      361 gatctattga cgtctgatct actgggtata aaaacccctt aattcctacc tataacctct
      421 ctttatcaaa aaagagacca aaaatggggc ccgtttcaaa gggagaggcc gttataaaag
      481 aatttatgcg tttcaaggtc cacatggagg gatccatgaa cggacacgag ttcgagatcg
      541 agggagaggg agagggacgt ccatacgagg gaacccaaac cgccaaggtg agttttatta
      601 tcgattttcg ggattttcta cttgtaatta ccttttgttt ttaataagtt gcttttttcc
      661 agctcaaggt caccaaggga ggaccactcc cattctcctg ggacatcctc tccccacaat
      721 tcatgtacgg atcccgtgcc ttcaccaagc acccagccga catcccagac tactacaagc
      781 aatccttccc agagggattc aagtgggagc gtgtcatgaa cttcgaggac ggaggagccg
      841 tcaccgtcac ccaaggtagg ttttcagttt caatttttgt tgcaactacc tcaactctat
      901 gttttcagac acctccctcg aggacggaac cctcatctac aaggtcaagc tccgtggaac
      961 caacttccca ccagacggac cagtcatgca aaagaagacc atgggatggg aggcctccac
     1021 cgagcgactc tacccagagg acggagtcct caagggagac atcaaggtaa atttttcaag
     1081 cttctaaatt ccaaaataaa ccattttaat ttcttaattc cagatggccc tccggctcaa
     1141 ggacggagga cgttacctcg ccgacttcaa gaccacctac aaggccaaga agccagtcca
     1201 aatgccagga gcctacaacg tcgaccgtaa gctcgacatc acctcccaca acgaggacta
     1261 caccgtcgtc gagcaatacg agcgttccga gggacgtcac tccaccggag gaatggacga
     1321 gctctacaag cccgggtgat ggtctctgag ctatgcaaaa tcctttcaag cattcccttc
     1381 ttctctatca ctcttctttc tttttgtcaa aaaattctct cgctaattta tttgcttttt
     1441 taatgttatt attttatgac tttttatagt cactgaaaag tttgcatctg agtgaagtga
     1501 atgctatcaa aatgtgattc tgatatccag gaaacggaga atctgtgcgg caatgtctta
     1561 tcatttgaaa cgcgccgtat cgatgctgac atcatgcggc tcccgtgtta gtcgagaaga
     1621 tgggtacggg cctttattca tccaacactc tacggcttct tcaagagcta gtagggcacc
     1681 ctgtagttgg aaagggatta tttcgtatgg cgagcccata ccgtctttct tgcggaagct
     1741 taacacgata ggaagttgga tagtttcgaa cgatggttcc atacggaacg ctgtctcaag
     1801 gatgagtctg acggagtgta actcgaaggc taggtggagg ctcagtgatg ataagtctgc
     1861 gatggtggat gcatgtgtca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     1921 gagggcacaa tcctattccg cgctatccga caatctccaa gacattaggt ggagttcagt
     1981 tcggcgtatg gcatatgtcg ctggaaagaa catgtgagca aaaggccagc aaaaggccag
     2041 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2101 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2161 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2221 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2281 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2341 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2401 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2461 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2521 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2581 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2641 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctctatt
     2701 caacaaagcc gccgtcccgt caagtcagcg taaatgggta gggggcttca aatcgtcctc
     2761 gtgataccaa ttcggagcct gcttttttgt acaaacttgt tgataatggc aattcaagga
     2821 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2881 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2941 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3001 agggcttacc atctggcccc agtgctgcaa tgataccgcg agagccacgc tcaccggctc
     3061 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3121 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3181 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3241 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3301 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3361 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3421 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3481 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3541 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3601 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3661 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3721 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3781 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3841 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccagat acctgaaaca
     3901 aaacccatcg tacggccaag gaagtctcca ataactgtga tccaccacaa gcgccagggt
     3961 tttcccagtc acgacgttgt aaaacgacgg ccagtcatgc ataatccgca cgcatctgga
     4021 ataaggaagt gccattccgc ctgacctaag tgcttttcga gatcgccaaa ccggagaacg
     4081 atcaggagaa tgatgagtcg ccacaggagt cggcaagata cgtgcgttat agatttgctc
     4141 caaacttttt gaaactcaaa acggtcggag caactgtgga attcaaagta ggagacatcc
     4201 caatccatca tttccgaatg atcgaacgtc acttcttcaa agatcgcctt ctgaagtgtt
     4261 ttgactttga attcggattc tgtattccga attcacgaaa caactgtgaa catatctatg
     4321 agttccctca actctctcaa caactcagtg agtcattatt ctaaaaagta caattcaaaa
     4381 gactaatctc tttttcagtg gacgacatga tcaacaatcc aaacgagact cgttctgaca
     4441 gcttctattt cgtcgataac aaactcgtca tgcacaacaa agccgactac tcatatgatg
     4501 cataaatctt cattgttgag tttatcttgt tgatttttga ataaattatc aactctttac
     4561 tttttaatgg gttatgaaat aaataaacat tgaaaactga taaacaacgt tcatctctat
     4621 aacttcgtat agcatacatt atacg
//
