LOCUS       sequence-397604-        4645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet BioPart for AWC neurons. (1) Mark neurons or (2) 
            cell-specific expression of transgenes (N or C-terminal fusions, or 
            untagged). Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4645)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH89 - [AWCp | wrmScarlet | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1893..1909)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1893..1909)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1906..1928)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2081..2669)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2161..2180)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2786..2810)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2910..3701,3702..3770))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3533..3552
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3771..3875)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3974..3996
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3988..4005
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3989..4005
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4629..4645,1..17))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 acattatacg aagttatgat atcaaaaatg attacgccaa gctgtaagtt taaacatgat
       61 cttactaact aactattctc atttaaattt tcagagctta aaaatggctg aaatcactca
      121 caacgatgga tacgctaatg ccaaccgaga tgtacctttt ttcaaagtgc agaaagtgaa
      181 tctaattaat ttgaagccaa actttgatgc atcaaaaaaa ctgccaacaa ctgctgatct
      241 tacgatggac tccaagaaaa acgtgacaat gtgtcgaaat ctttgaaatc tcgtttttga
      301 gtgagctctt tcaaacaaaa gcgaaagctt ctactcattc aattttaaaa gtttgaataa
      361 tattaatagt attacgcaat aatcccggat tagactcact ataaaaagta gtccaaagtt
      421 agcagtagca catcaagaaa aaagagaaca aaaatggggc ccgtttcaaa gggagaggcc
      481 gttataaaag aatttatgcg tttcaaggtc cacatggagg gatccatgaa cggacacgag
      541 ttcgagatcg agggagaggg agagggacgt ccatacgagg gaacccaaac cgccaaggtg
      601 agttttatta tcgattttcg ggattttcta cttgtaatta ccttttgttt ttaataagtt
      661 gcttttttcc agctcaaggt caccaaggga ggaccactcc cattctcctg ggacatcctc
      721 tccccacaat tcatgtacgg atcccgtgcc ttcaccaagc acccagccga catcccagac
      781 tactacaagc aatccttccc agagggattc aagtgggagc gtgtcatgaa cttcgaggac
      841 ggaggagccg tcaccgtcac ccaaggtagg ttttcagttt caatttttgt tgcaactacc
      901 tcaactctat gttttcagac acctccctcg aggacggaac cctcatctac aaggtcaagc
      961 tccgtggaac caacttccca ccagacggac cagtcatgca aaagaagacc atgggatggg
     1021 aggcctccac cgagcgactc tacccagagg acggagtcct caagggagac atcaaggtaa
     1081 atttttcaag cttctaaatt ccaaaataaa ccattttaat ttcttaattc cagatggccc
     1141 tccggctcaa ggacggagga cgttacctcg ccgacttcaa gaccacctac aaggccaaga
     1201 agccagtcca aatgccagga gcctacaacg tcgaccgtaa gctcgacatc acctcccaca
     1261 acgaggacta caccgtcgtc gagcaatacg agcgttccga gggacgtcac tccaccggag
     1321 gaatggacga gctctacaag cccgggtgat ggtctctgag ctatgcaaaa tcctttcaag
     1381 cattcccttc ttctctatca ctcttctttc tttttgtcaa aaaattctct cgctaattta
     1441 tttgcttttt taatgttatt attttatgac tttttatagt cactgaaaag tttgcatctg
     1501 agtgaagtga atgctatcaa aatgtgattc tgatatccag gaaacggaga atctgtgcgg
     1561 caatgtctta tcatttgaaa cgcgccgtat cgatgctgac atcatgcggc tcccgtgtta
     1621 gtcgagaaga tgggtacggg cctttattca tccaacactc tacggcttct tcaagagcta
     1681 gtagggcacc ctgtagttgg aaagggatta tttcgtatgg cgagcccata ccgtctttct
     1741 tgcggaagct taacacgata ggaagttgga tagtttcgaa cgatggttcc atacggaacg
     1801 ctgtctcaag gatgagtctg acggagtgta actcgaaggc taggtggagg ctcagtgatg
     1861 ataagtctgc gatggtggat gcatgtgtca tggtcatagc tgtttcctgt gtgaaattgt
     1921 tatccgctca gagggcacaa tcctattccg cgctatccga caatctccaa gacattaggt
     1981 ggagttcagt tcggcgtatg gcatatgtcg ctggaaagaa catgtgagca aaaggccagc
     2041 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2101 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2161 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2221 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2281 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2341 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2401 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2461 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2521 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2581 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2641 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2701 acgctctatt caacaaagcc gccgtcccgt caagtcagcg taaatgggta gggggcttca
     2761 aatcgtcctc gtgataccaa ttcggagcct gcttttttgt acaaacttgt tgataatggc
     2821 aattcaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2881 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2941 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3001 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agagccacgc
     3061 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3121 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3181 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3241 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3301 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3361 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3421 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3481 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3541 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3601 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3661 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3721 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3781 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3841 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccagat
     3901 acctgaaaca aaacccatcg tacggccaag gaagtctcca ataactgtga tccaccacaa
     3961 gcgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtcatgc ataatccgca
     4021 cgcatctgga ataaggaagt gccattccgc ctgacctaag tgcttttcga gatcgccaaa
     4081 ccggagaacg atcaggagaa tgatgagtcg ccacaggagt cggcaagata cgtgcgttat
     4141 agatttgctc caaacttttt gaaactcaaa acggtcggag caactgtgga attcaaagta
     4201 ggagacatcc caatccatca tttccgaatg atcgaacgtc acttcttcaa agatcgcctt
     4261 ctgaagtgtt ttgactttga attcggattc tgtattccga attcacgaaa caactgtgaa
     4321 catatctatg agttccctca actctctcaa caactcagtg agtcattatt ctaaaaagta
     4381 caattcaaaa gactaatctc tttttcagtg gacgacatga tcaacaatcc aaacgagact
     4441 cgttctgaca gcttctattt cgtcgataac aaactcgtca tgcacaacaa agccgactac
     4501 tcatatgatg cataaatctt cattgttgag tttatcttgt tgatttttga ataaattatc
     4561 aactctttac tttttaatgg gttatgaaat aaataaacat tgaaaactga taaacaacgt
     4621 tcatctctat aacttcgtat agcat
//
