LOCUS       sequence-397607-        9553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses APEX2-G3BP1 in the mammalian cytosol under dox inducible 
            promoter, lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Qin W, Cheah JS, Xu C, Messing J, Freibaum BD, Boeynaems S, Taylor 
            JP, Udeshi ND, Carr SA, Ting AY
  TITLE     Dynamic mapping of proteome trafficking within and between living 
            cells by TransitID.
  JOURNAL   Cell. 2023 Jun 23:S0092-8674(23)00596-2. doi: 
            10.1016/j.cell.2023.05.044.
  PUBMED    37385249
REFERENCE   2  (bases 1 to 9553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2023 
            Jun 23:S0092-8674(23)00596-2. doi: 10.1016/j.cell.2023.05.044."
COMMENT     Sequence Label: APEX2-V5-G3BP1_pTRE-G418
FEATURES             Location/Qualifiers
     source          1..9553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2805..2922
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2976..3290
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2984..3002
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3020..3038
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3055..3073
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3091..3109
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3127..3145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3162..3180
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3198..3216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3263..3287
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3307..3316
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4072..4113
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        5538..6048
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5967..5985
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(6058..6675,6676..6681,6682..6717,6718..6720,6721..6756,
                     6757..6759,6760..6795,6796..6801)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             6811..6867
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6868..7659
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6868..7659
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6919..6938)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7529..7548
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             7850..8083
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8155..8276
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8192..8211)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8246..8265
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8316..8451
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8451..8522
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8523..8591,8592..9383)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8741..8760)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacaattct cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2821 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2881 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2941 agcagagatc cactttggcc gcgaatcgat atgtcgagtt tactccctat cagtgataga
     3001 gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt ttactcccta
     3061 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     3121 gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca gtgatagaga
     3181 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac
     3241 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagaattg
     3301 gctagcgcca ccatggggaa atcataccca acagtgtccg cagactacca ggatgccgtg
     3361 gagaaagcca agaagaagct gagagggttt atcgcagaaa agaggtgcgc acctctgatg
     3421 ctgagactgg ctttccacag cgcaggcacc tttgacaagg gaaccaaaac aggcggaccc
     3481 tttggaacaa tcaagcaccc tgctgaactg gcacatagtg ccaacaatgg gctggacatc
     3541 gccgtgcggc tgctggaacc tctgaaagca gagttcccaa ttctgtccta cgccgatttt
     3601 tatcagctgg caggagtggt cgctgtggag gtcactgggg gccccaaggt gcctttccac
     3661 ccaggacggg aggacaaacc agaaccacct ccagaggggc gcctgccaga tccgacaaag
     3721 ggctccgacc atctgcgaga tgtgtttggg aaagctatgg gcctgactga ccaggatatc
     3781 gtcgcactgt ctggagggca caccattggc gccgctcata aggaaaggtc aggcttcgag
     3841 ggaccctgga caagcaaccc cctgattttc gacaattctt actttactga actgctgagt
     3901 ggagagaagg aagggctgct gcagctgccc agcgacaaag ccctgctgtc cgatcccgtg
     3961 ttcagacctc tggtcgataa gtatgcagcc gacgaggatg cttttttcgc agattacgca
     4021 gaagcacatc agaagctgtc agaactggga tttgccgacg ccgggcgcgc cggcaagccc
     4081 atccccaacc ccctgctggg cctggacagc accggatcca tggtgatgga gaagcccagc
     4141 cctctactag taggccggga atttgtgaga cagtattaca cactgctgaa ccaggcccca
     4201 gacatgctgc atagatttta tggaaagaac tcttcttatg tccatggggg attggattca
     4261 aatggaaagc cagcagatgc agtctacgga cagaaagaaa tccacaggaa agtgatgtca
     4321 caaaacttca ccaactgcca caccaagatt cgccatgttg atgctcatgc cacgctaaat
     4381 gatggtgtgg tagtccaggt gatggggctt ctctctaaca acaaccaggc tttgaggaga
     4441 ttcatgcaaa cgtttgtcct tgctcctgag gggtctgttg caaataaatt ctatgttcac
     4501 aatgatatct tcagatacca agatgaggtc tttggtgggt ttgtcactga gcctcaggag
     4561 gagtctgaag aagaagtaga ggaacctgaa gaaagacagc aaacacctga ggtggtacct
     4621 gatgattctg gaactttcta tgatcaggca gttgtcagta atgacatgga agaacattta
     4681 gaggagcctg ttgctgaacc agagcctgat cctgaaccag aaccagaaca agaacctgta
     4741 tctgaaatcc aagaggaaaa gcctgagcca gtattagaag aaactgcccc tgaggatgct
     4801 cagaagagtt cttctccagc acctgcagac atagctcaga cagtacagga agacttgagg
     4861 acattttctt gggcatctgt gaccagtaag aatcttccac ccagtggagc tgttccagtt
     4921 actgggatac cacctcatgt tgttaaagta ccagcttcac agccccgtcc agagtctaag
     4981 cctgaatctc agattccacc acaaagacct cagcgggatc aaagagtgcg agaacaacga
     5041 ataaatattc ctccccaaag gggacccaga ccaatccgtg aggctggtga gcaaggtgac
     5101 attgaacccc gaagaatggt gagacaccct gacagtcacc aactcttcat tggcaacctg
     5161 cctcatgaag tggacaaatc agagcttaaa gatttctttc aaagttatgg aaacgtggtg
     5221 gagttgcgca ttaacagtgg tgggaaatta cccaattttg gttttgttgt gtttgatgat
     5281 tctgagcctg ttcagaaagt ccttagcaac agacccatca tgttcagagg tgaggtccgt
     5341 ctgaatgtcg aagagaagaa gactcgagct gccagggaag gcgaccgacg agataatcgc
     5401 cttcggggac ctggaggccc tcgaggtggg ctgggtggtg gaatgagagg ccctccccgt
     5461 ggaggcatgg tgcagaaacc aggatttgga gtgggaaggg ggcttgcgcc acggcagtaa
     5521 tagggccgaa ttcatccggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg
     5581 cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct
     5641 cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc
     5701 cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg
     5761 tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc
     5821 cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca
     5881 gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta
     5941 gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg
     6001 tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccagca attcaccatg
     6061 tctagactgg acaagagcaa agtcataaac ggagctctgg aattactcaa tggtgtcggt
     6121 atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg
     6181 tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac
     6241 aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac
     6301 aacgccaagt cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc
     6361 ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt
     6421 cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca
     6481 ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct
     6541 accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga ccggcaggga
     6601 gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta
     6661 aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca
     6721 gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt
     6781 gaccttgaca tgctccccgg gggaagcgga gctactaact tcagcctgct gaagcaggct
     6841 ggagacgtgg aggagaaccc tggacctatt gaacaagatg gattgcacgc aggttctccg
     6901 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     6961 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     7021 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     7081 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     7141 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     7201 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     7261 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     7321 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     7381 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     7441 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     7501 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     7561 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     7621 cgcatcgcct tctatcgcct tcttgacgag ttcttctgat aaccgcggcc tgctgccggc
     7681 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7741 cgcctccccg cctgtttcgc ctcggcgtcc ggactagagg tacctttaag accaatgact
     7801 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7861 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     7921 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7981 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8041 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     8101 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     8161 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8221 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8281 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     8341 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8401 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctttcaaata
     8461 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     8521 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     8581 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     8641 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     8701 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     8761 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     8821 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8881 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8941 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     9001 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     9061 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     9121 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     9181 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     9241 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     9301 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     9361 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     9421 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     9481 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     9541 tcaaaggatc ttc
//
