LOCUS       sequence-397608-        4645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet BioPart for ASEL neurons. (1) Mark neurons or (2) 
            cell-specific expression of transgenes (N or C-terminal fusions, or 
            untagged). Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4645)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH59 - [ASELp | wrmScarlet | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1871..1887)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1871..1887)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1884..1906)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2059..2647)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2139..2158)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2764..2788)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2888..3679,3680..3748))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3511..3530
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3749..3853)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3952..3974
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3966..3983
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3967..3983
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4607..4640)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 caaaaatgat tacgccaagc tgtaagttta aacatgatct tactaactaa ctattctcat
       61 ttaaattttc agagcttaaa aatggctgaa atcactcaca acgatggata cgctaatgcc
      121 cctagaaatg agaagtgcat cctaaacaca tccgatgcat cccagacaat tcgtaatttt
      181 ctttctgact aatgaagctg attaatacgc gtataggact gaacaaatat attatctctg
      241 acttgttcgt cgaagccctt caacaagttt gctcatgatt atgagccgcc tggaatatat
      301 ccgaaaacta ataaaaacta aagaaaacaa gatagaatat tgtttttata acactttttg
      361 tatatattaa aacctcagaa gactgcccac ttattcattt tcataactcc tttccaaaaa
      421 agagaccaaa aatggggccc gtttcaaagg gagaggccgt tataaaagaa tttatgcgtt
      481 tcaaggtcca catggaggga tccatgaacg gacacgagtt cgagatcgag ggagagggag
      541 agggacgtcc atacgaggga acccaaaccg ccaaggtgag ttttattatc gattttcggg
      601 attttctact tgtaattacc ttttgttttt aataagttgc ttttttccag ctcaaggtca
      661 ccaagggagg accactccca ttctcctggg acatcctctc cccacaattc atgtacggat
      721 cccgtgcctt caccaagcac ccagccgaca tcccagacta ctacaagcaa tccttcccag
      781 agggattcaa gtgggagcgt gtcatgaact tcgaggacgg aggagccgtc accgtcaccc
      841 aaggtaggtt ttcagtttca atttttgttg caactacctc aactctatgt tttcagacac
      901 ctccctcgag gacggaaccc tcatctacaa ggtcaagctc cgtggaacca acttcccacc
      961 agacggacca gtcatgcaaa agaagaccat gggatgggag gcctccaccg agcgactcta
     1021 cccagaggac ggagtcctca agggagacat caaggtaaat ttttcaagct tctaaattcc
     1081 aaaataaacc attttaattt cttaattcca gatggccctc cggctcaagg acggaggacg
     1141 ttacctcgcc gacttcaaga ccacctacaa ggccaagaag ccagtccaaa tgccaggagc
     1201 ctacaacgtc gaccgtaagc tcgacatcac ctcccacaac gaggactaca ccgtcgtcga
     1261 gcaatacgag cgttccgagg gacgtcactc caccggagga atggacgagc tctacaagcc
     1321 cgggtgatgg tctctgagct atgcaaaatc ctttcaagca ttcccttctt ctctatcact
     1381 cttctttctt tttgtcaaaa aattctctcg ctaatttatt tgctttttta atgttattat
     1441 tttatgactt tttatagtca ctgaaaagtt tgcatctgag tgaagtgaat gctatcaaaa
     1501 tgtgattctg atatccagga aacggagaat ctgtgcggca atgtcttatc atttgaaacg
     1561 cgccgtatcg atgctgacat catgcggctc ccgtgttagt cgagaagatg ggtacgggcc
     1621 tttattcatc caacactcta cggcttcttc aagagctagt agggcaccct gtagttggaa
     1681 agggattatt tcgtatggcg agcccatacc gtctttcttg cggaagctta acacgatagg
     1741 aagttggata gtttcgaacg atggttccat acggaacgct gtctcaagga tgagtctgac
     1801 ggagtgtaac tcgaaggcta ggtggaggct cagtgatgat aagtctgcga tggtggatgc
     1861 atgtgtcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaga gggcacaatc
     1921 ctattccgcg ctatccgaca atctccaaga cattaggtgg agttcagttc ggcgtatggc
     1981 atatgtcgct ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2041 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2101 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2161 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2221 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2281 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2341 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2401 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2461 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2521 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2581 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2641 atctcaagaa gatcctttga tcttttctac ggggtctgac gctctattca acaaagccgc
     2701 cgtcccgtca agtcagcgta aatgggtagg gggcttcaaa tcgtcctcgt gataccaatt
     2761 cggagcctgc ttttttgtac aaacttgttg ataatggcaa ttcaaggatc ttcacctaga
     2821 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2881 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2941 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3001 ctggccccag tgctgcaatg ataccgcgag agccacgctc accggctcca gatttatcag
     3061 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3121 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3181 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3241 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3301 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3361 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3421 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3481 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3541 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3601 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3661 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3721 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3781 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3841 taggggttcc gcgcacattt ccccgaaaag tgccagatac ctgaaacaaa acccatcgta
     3901 cggccaagga agtctccaat aactgtgatc caccacaagc gccagggttt tcccagtcac
     3961 gacgttgtaa aacgacggcc agtcatgcat aatccgcacg catctggaat aaggaagtgc
     4021 cattccgcct gacctaagtg cttttcgaga tcgccaaacc ggagaacgat caggagaatg
     4081 atgagtcgcc acaggagtcg gcaagatacg tgcgttatag atttgctcca aactttttga
     4141 aactcaaaac ggtcggagca actgtggaat tcaaagtagg agacatccca atccatcatt
     4201 tccgaatgat cgaacgtcac ttcttcaaag atcgccttct gaagtgtttt gactttgaat
     4261 tcggattctg tattccgaat tcacgaaaca actgtgaaca tatctatgag ttccctcaac
     4321 tctctcaaca actcagtgag tcattattct aaaaagtaca attcaaaaga ctaatctctt
     4381 tttcagtgga cgacatgatc aacaatccaa acgagactcg ttctgacagc ttctatttcg
     4441 tcgataacaa actcgtcatg cacaacaaag ccgactactc atatgatgca taaatcttca
     4501 ttgttgagtt tatcttgttg atttttgaat aaattatcaa ctctttactt tttaatgggt
     4561 tatgaaataa ataaacattg aaaactgata aacaacgttc atctctataa cttcgtatag
     4621 catacattat acgaagttat gatat
//
