LOCUS       sequence-397609-        6876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soma and AIS-localized genetically encoded voltage indicator (GEVI) 
            JEDI-1P in AAV production vector for expression in excitatory 
            glutamatergic neurons using the promoter CaMKII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Lu X, Wang Y, Liu Z, Gou Y, Jaeger D, St-Pierre F
  TITLE     Widefield imaging of rapid pan-cortical voltage dynamics with an 
            indicator evolved for one-photon microscopy.
  JOURNAL   Nat Commun. 2023 Oct 12;14(1):6423. doi: 10.1038/s41467-023-41975-3.
  PUBMED    37828037
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-10-12"; volume: "14(1):6423. doi"; pages: " 
            10.1038/s41467-023-41975-3"
COMMENT     Sequence Label: pAAV-CaMKII-JEDI-1P-Kv-WPRE
FEATURES             Location/Qualifiers
     source          1..6876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        149..1437
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      1458..1467
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3003..3591
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3056..3076)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3474..3485)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3623..4099
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3822..3841)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4139..4279
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4139..4268
                     /label=AAV2 ITR
     rep_origin      4354..4809
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4441..4460)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4651..4672
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4826..4845)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4945..4967
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5005..5023)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5091..5195
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5196..5264,5265..6056)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5414..5433)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6227..6815
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6716..6735
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtttaaca ttatggcctt aggtcacttc atctccatgg
      181 ggttcttctt ctgattttct agaaaatgag atgggggtgc agagagcttc ctcagtgacc
      241 tgcccagggt cacatcagaa atgtcagagc tagaacttga actcagatta ctaatcttaa
      301 attccatgcc ttgggggcat gcaagtacga tatacagaag gagtgaactc attagggcag
      361 atgaccaatg agtttaggaa agaagagtcc agggcagggt acatctacac cacccgccca
      421 gccctgggtg agtccagcca cgttcacctc attatagttg cctctctcca gtcctacctt
      481 gacgggaagc acaagcagaa actgggacag gagccccagg agaccaaatc ttcatggtcc
      541 ctctgggagg atgggtgggg agagctgtgg cagaggcctc aggaggggcc ctgctgctca
      601 gtggtgacag ataggggtga gaaagcagac agagtcattc cgtcagcatt ctgggtctgt
      661 ttggtacttc ttctcacgct aaggtggcgg tgtgatatgc acaatggcta aaaagcaggg
      721 agagctggaa agaaacaagg acagagacag aggccaagtc aaccagacca attcccagag
      781 gaagcaaaga aaccattaca gagactacaa gggggaaggg aaggagagat gaattagctt
      841 cccctgtaaa ccttagaacc cagctgttgc cagggcaacg gggcaatacc tgtctcttca
      901 gaggagatga agttgccagg gtaactacat cctgtctttc tcaaggacca tcccagaatg
      961 tggcacccac tagccgttac catagcaact gcctctttgc cccacttaat cccatcccgt
     1021 ctgttaaaag ggccctatag ttggaggtgg gggaggtagg aagagcgatg atcacttgtg
     1081 gactaagttt gttcgcatcc ccttctccaa ccccctcagt acatcaccct gggggaacag
     1141 ggtccacttg ctcctgggcc cacacagtcc tgcagtattg tgtatataag gccagggcaa
     1201 agaggagcag gttttaaagt gaaaggcagg caggtgttgg ggaggcagtt accggggcaa
     1261 cgggaacagg gcgtttcgga ggtggttgcc atggggacct ggatgctgac gaaggctcgc
     1321 gaggctgtga gcagccacag tgccctgctc agaagcccca agctcgtcag tcaagccggt
     1381 tctccgtttg cactcaggag cacgggcagg cgagtggccc ctagttctgg gggcagctct
     1441 agagcggtac cggatccgcc accatggaga cgactgtgag gtatgaacag gggtcagagc
     1501 tcactaaaac ttcgagctct ccaacagcag atgagcccac gataaagatt gatgatggtc
     1561 gtgatgaggg taatgaacaa gacagctgtt ccaataccat taggagaaaa atttccccgt
     1621 ttgtgatgtc atttggattc agagtatttg gagttgtgct tatcattgta gacatcatag
     1681 tggtgattgt ggatctggcc atcagtgaga agaaaagagg cattagagag attcttgaag
     1741 gtgtttccct ggctatagca ctcttcttcc ttgttgatgt tctcatgaga gtgtttgttg
     1801 aaggcttcaa gaactatttc cggtccaaac tggttacttt ggatgcagtc atagtagtgg
     1861 gcactctgct aattaatatg acctactcct tctctgacct tgctgccttt aacagcgaga
     1921 acgtgtatat taccgcggat aaacagaaaa acggcattaa agcgaacttt accgtgcgcc
     1981 ataacgtgga agatggcagc gtgcagctgg cggatcatta tcagcagaac accccgattg
     2041 gcgatggccc ggtgctgctg ccggataacc attatctgag cacccagacc gtgctgagca
     2101 aagatccgaa cgaaaaacgc gatcacatgg tgctgctgga atttgtgacc gcagcgggca
     2161 ttacacacgg catggatgaa ctgtatggcg gcaccggcgg cagcgcgagc cagggcgaag
     2221 aactgtttac cggcgtggtg ccgattctgg tggaactgga tggcgatgtg aacggccata
     2281 aatttagcgt gcgcggcgaa ggcgaaggcg atgcgaccat tggcaaactg accctgaaat
     2341 ttatttgcac caccggcaaa ctaccggtgc cgtggccgac cctggtgacc accttaacct
     2401 atggcgtgca gtgctttagc cgctatccgg atcatatgaa acgccatgat ttttttaaaa
     2461 gcgcgatgcc ggaaggctat gtgcaggaac gcaccattag ctttaaagat gatggcaaat
     2521 ataaaacccg cgcggtggtg aaatttgaag gcgataccct ggtgaaccgc attgaactga
     2581 aaggcaccga ttttaaagaa gatggcaaca ttctggggca taaactggaa tataacacag
     2641 atcagatgcc gcacatggtt actcttcttc gagttctgaa aattgttatc ttaataagaa
     2701 tatttcgcct ggcttcacag aagaaacaac ttgaagtggt aaccggttct tcaggttcat
     2761 ctggatcttc cacgcgtcag tcccagccca tcctcaacac caaggagatg gccccgcaga
     2821 gcaagcctcc agaggagctg gagatgagca gcatgcccag ccccgtggcc cctctgcccg
     2881 cacgcacgga gggcgtcatc gacatgcgga gcatgtccag cattgacagc ttcatcagct
     2941 gtgccacgga cttccctgaa gccaccagat tctgataaga attcgatatc aagcttatcg
     3001 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3061 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3121 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     3181 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3241 ctggttgggg cattgccacc acctgtcagc tcctttccgg aactttcgct ttccccctcc
     3301 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3361 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     3421 tcgcctatgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     3481 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     3541 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     3601 gagcgctgct cgagagatct acgggtggca tccctgtgac ccctccccag tgcctctcct
     3661 ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat
     3721 cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag
     3781 caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg
     3841 gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct
     3901 cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt
     3961 tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta
     4021 atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac
     4081 tgctcccttc cctgtccttc tgattttgta ggtaaccacg tgcggaccga gcggccgcag
     4141 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     4201 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     4261 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     4321 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     4381 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
     4441 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4501 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4561 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4621 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4681 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
     4741 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     4801 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     4861 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     4921 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     4981 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5041 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5101 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5161 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5221 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5281 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5341 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5401 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5461 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5521 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5581 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5641 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5701 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5761 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5821 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5881 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5941 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6001 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6061 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6121 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6181 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6241 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6301 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6361 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6421 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6481 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6541 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6601 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6661 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6721 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6781 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6841 ggttcctggc cttttgctgg ccttttgctc acatgt
//
