LOCUS       sequence-397610-        4645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet BioPart for DD neurons. (1) Mark neurons or (2) 
            cell-specific expression of transgenes (N or C-terminal fusions, or 
            untagged). Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4645)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH51 - [DDp | wrmScarlet | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1901..1917)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1901..1917)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1914..1936)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2089..2677)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2169..2188)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2794..2818)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2918..3709,3710..3778))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3541..3560
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3779..3883)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3982..4004
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3996..4013
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3997..4013
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4637..4645,1..25))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tatagcatac attatacgaa gttatgatat caaaaatgat tacgccaagc tgtaagttta
       61 aacatgatct tactaactaa ctattctcat ttaaattttc agagcttaaa aatggctgaa
      121 atcactcaca acgatggata cgctaatgcc ttctccagaa tgtttttggt ttctgatcga
      181 taaatgttta ctggttgttg atgtttgtaa aagtcctgca aattctagaa aattctcaaa
      241 ctctttttct tattttcatg agaaaaccga aactttgaag tacataagca aagagttgcg
      301 ttgttatttt aatcttttga acctattaat aaatcttttt tgttttctcg ggattatgtt
      361 aggattacaa agacatttaa gacaagaagt tgtttctgtt atttcacttt ttttgtttgc
      421 gcattcacaa ttttgttaaa acaaaaaaaa agagaccaaa aatggggccc gtttcaaagg
      481 gagaggccgt tataaaagaa tttatgcgtt tcaaggtcca catggaggga tccatgaacg
      541 gacacgagtt cgagatcgag ggagagggag agggacgtcc atacgaggga acccaaaccg
      601 ccaaggtgag ttttattatc gattttcggg attttctact tgtaattacc ttttgttttt
      661 aataagttgc ttttttccag ctcaaggtca ccaagggagg accactccca ttctcctggg
      721 acatcctctc cccacaattc atgtacggat cccgtgcctt caccaagcac ccagccgaca
      781 tcccagacta ctacaagcaa tccttcccag agggattcaa gtgggagcgt gtcatgaact
      841 tcgaggacgg aggagccgtc accgtcaccc aaggtaggtt ttcagtttca atttttgttg
      901 caactacctc aactctatgt tttcagacac ctccctcgag gacggaaccc tcatctacaa
      961 ggtcaagctc cgtggaacca acttcccacc agacggacca gtcatgcaaa agaagaccat
     1021 gggatgggag gcctccaccg agcgactcta cccagaggac ggagtcctca agggagacat
     1081 caaggtaaat ttttcaagct tctaaattcc aaaataaacc attttaattt cttaattcca
     1141 gatggccctc cggctcaagg acggaggacg ttacctcgcc gacttcaaga ccacctacaa
     1201 ggccaagaag ccagtccaaa tgccaggagc ctacaacgtc gaccgtaagc tcgacatcac
     1261 ctcccacaac gaggactaca ccgtcgtcga gcaatacgag cgttccgagg gacgtcactc
     1321 caccggagga atggacgagc tctacaagcc cgggtgatgg tctctgagct atgcaaaatc
     1381 ctttcaagca ttcccttctt ctctatcact cttctttctt tttgtcaaaa aattctctcg
     1441 ctaatttatt tgctttttta atgttattat tttatgactt tttatagtca ctgaaaagtt
     1501 tgcatctgag tgaagtgaat gctatcaaaa tgtgattctg atatccagga aacggagaat
     1561 ctgtgcggca atgtcttatc atttgaaacg cgccgtatcg atgctgacat catgcggctc
     1621 ccgtgttagt cgagaagatg ggtacgggcc tttattcatc caacactcta cggcttcttc
     1681 aagagctagt agggcaccct gtagttggaa agggattatt tcgtatggcg agcccatacc
     1741 gtctttcttg cggaagctta acacgatagg aagttggata gtttcgaacg atggttccat
     1801 acggaacgct gtctcaagga tgagtctgac ggagtgtaac tcgaaggcta ggtggaggct
     1861 cagtgatgat aagtctgcga tggtggatgc atgtgtcatg gtcatagctg tttcctgtgt
     1921 gaaattgtta tccgctcaga gggcacaatc ctattccgcg ctatccgaca atctccaaga
     1981 cattaggtgg agttcagttc ggcgtatggc atatgtcgct ggaaagaaca tgtgagcaaa
     2041 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2101 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2161 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2221 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2281 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2341 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2401 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2461 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2521 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2581 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2641 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2701 ggggtctgac gctctattca acaaagccgc cgtcccgtca agtcagcgta aatgggtagg
     2761 gggcttcaaa tcgtcctcgt gataccaatt cggagcctgc ttttttgtac aaacttgttg
     2821 ataatggcaa ttcaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2881 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2941 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3001 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3061 agccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3121 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3181 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3241 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3301 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3361 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3421 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3481 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3541 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3601 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3661 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3721 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3781 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3841 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3901 tgccagatac ctgaaacaaa acccatcgta cggccaagga agtctccaat aactgtgatc
     3961 caccacaagc gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtcatgcat
     4021 aatccgcacg catctggaat aaggaagtgc cattccgcct gacctaagtg cttttcgaga
     4081 tcgccaaacc ggagaacgat caggagaatg atgagtcgcc acaggagtcg gcaagatacg
     4141 tgcgttatag atttgctcca aactttttga aactcaaaac ggtcggagca actgtggaat
     4201 tcaaagtagg agacatccca atccatcatt tccgaatgat cgaacgtcac ttcttcaaag
     4261 atcgccttct gaagtgtttt gactttgaat tcggattctg tattccgaat tcacgaaaca
     4321 actgtgaaca tatctatgag ttccctcaac tctctcaaca actcagtgag tcattattct
     4381 aaaaagtaca attcaaaaga ctaatctctt tttcagtgga cgacatgatc aacaatccaa
     4441 acgagactcg ttctgacagc ttctatttcg tcgataacaa actcgtcatg cacaacaaag
     4501 ccgactactc atatgatgca taaatcttca ttgttgagtt tatcttgttg atttttgaat
     4561 aaattatcaa ctctttactt tttaatgggt tatgaaataa ataaacattg aaaactgata
     4621 aacaacgttc atctctataa cttcg
//
