LOCUS       sequence-397612-        4404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector for mosTI(unc-119, gfp). MCS or Golden Gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4404)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH46 - MosTI [unc-119(partial) | MCS | gfp]
FEATURES             Location/Qualifiers
     source          1..4404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1652..1668)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1652..1668)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1665..1687)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1840..2428)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1920..1939)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2545..2569)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2669..3460,3461..3529))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3292..3311
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3530..3634)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3733..3755
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3747..3764
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3748..3764
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4388..4404,1..17))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 acattatacg aagttatgat atcaaaaatg attacgccaa gctgtaagtt taaacatgat
       61 cttactaact aactattctc atttaaattt tcagagctta aaaatggctg aaatcactca
      121 caacgatgga tacgctaatg ccagagacga gtgcacagct agcaataccg agctcactgc
      181 agacgtctct aaaaatgggg ccctctaagg gcgaagaatt gttcacggga gtagtcccaa
      241 tcttagtcga gcttgacgga ggtgagtttt attatcgatt ttcgggattt tctacttgta
      301 attacctttt gtttttaata agttgctttt ttccagatgt taatggccac aagttttccg
      361 tgtctggaga aggagaaggc gacgctacct acggtaagct gaccctgaag ttcatttgca
      421 ctactggcaa gttaccagta ccatggccga ccctggtgac aacattttgc tacggggttc
      481 aatgcttctc cagataccca ggtaggtttt cagtttcaat ttttgttgca actacctcaa
      541 ctctatgttt tcagatcata tgaaaagaca cgactttttt aaatccgcca tgccagaggg
      601 ttacgttcaa gaacgtacca ttttttttaa ggacgatggt aactacaaga cacgtgcgga
      661 ggttaaattt gaaggcgata ctctcgtgaa ccgtattgag ctcaaggtaa atttttcaag
      721 cttctaaatt ccaaaataaa ccattttaat ttcttaattc cagggaatcg atttcaagga
      781 agatggaaac attttgggcc acaagcttga atataattat aatagtcata atgtctacat
      841 tatggccgac aaacaaaaaa acggtattaa ggttaacttc aagattcgcc acaatatcga
      901 agatggatct gtccaacttg ctgaccacta ccaacaaaat actccaattg gagatggacc
      961 ggttttgctt ccagataacc actacctttc cactcagtct gccctctcta aggatccgaa
     1021 tgaaaaacgt gaccatatgg ttcttttgga gttcgttacc gcagcaggaa ttactcacgg
     1081 aatggatgaa ctgtacaagc ccgggtgatg gtctctgagc tatgcaaaat cctttcaagc
     1141 attcccttct tctctatcac tcttctttct ttttgtcaaa aaattctctc gctaatttat
     1201 ttgctttttt aatgttatta ttttatgact ttttatagtc actgaaaagt ttgcatctga
     1261 gtgaagtgaa tgctatcaaa atgtgattct gatatccagg aaacggagaa tctgtgcggc
     1321 aatgtcttat catttgaaac gcgccgtatc gatgctgaca tcatgcggct cccgtgttag
     1381 tcgagaagat gggtacgggc ctttattcat ccaacactct acggcttctt caagagctag
     1441 tagggcaccc tgtagttgga aagggattat ttcgtatggc gagcccatac cgtctttctt
     1501 gcggaagctt aacacgatag gaagttggat agtttcgaac gatggttcca tacggaacgc
     1561 tgtctcaagg atgagtctga cggagtgtaa ctcgaaggct aggtggaggc tcagtgatga
     1621 taagtctgcg atggtggatg catgtgtcat ggtcatagct gtttcctgtg tgaaattgtt
     1681 atccgctcag agggcacaat cctattccgc gctatccgac aatctccaag acattaggtg
     1741 gagttcagtt cggcgtatgg catatgtcgc tggaaagaac atgtgagcaa aaggccagca
     1801 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1861 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1921 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1981 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2041 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2101 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2161 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2221 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2281 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2341 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2401 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2461 cgctctattc aacaaagccg ccgtcccgtc aagtcagcgt aaatgggtag ggggcttcaa
     2521 atcgtcctcg tgataccaat tcggagcctg cttttttgta caaacttgtt gataatggca
     2581 attcaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2641 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2701 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     2761 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gagccacgct
     2821 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     2881 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     2941 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3001 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     3061 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     3121 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     3181 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     3241 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     3301 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3361 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3421 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3481 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3541 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3601 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccagata
     3661 cctgaaacaa aacccatcgt acggccaagg aagtctccaa taactgtgat ccaccacaag
     3721 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtcatgca taatccgcac
     3781 gcatctggaa taaggaagtg ccattccgcc tgacctaagt gcttttcgag atcgccaaac
     3841 cggagaacga tcaggagaat gatgagtcgc cacaggagtc ggcaagatac gtgcgttata
     3901 gatttgctcc aaactttttg aaactcaaaa cggtcggagc aactgtggaa ttcaaagtag
     3961 gagacatccc aatccatcat ttccgaatga tcgaacgtca cttcttcaaa gatcgccttc
     4021 tgaagtgttt tgactttgaa ttcggattct gtattccgaa ttcacgaaac aactgtgaac
     4081 atatctatga gttccctcaa ctctctcaac aactcagtga gtcattattc taaaaagtac
     4141 aattcaaaag actaatctct ttttcagtgg acgacatgat caacaatcca aacgagactc
     4201 gttctgacag cttctatttc gtcgataaca aactcgtcat gcacaacaaa gccgactact
     4261 catatgatgc ataaatcttc attgttgagt ttatcttgtt gatttttgaa taaattatca
     4321 actctttact ttttaatggg ttatgaaata aataaacatt gaaaactgat aaacaacgtt
     4381 catctctata acttcgtata gcat
//
