LOCUS       sequence-397613-        4645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet BioPart for PVT neurons. (1) Mark neurons or (2) 
            cell-specific expression of transgenes (N or C-terminal fusions, or 
            untagged). Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4645)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH81 - [PVTp | wrmScarlet | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1908..1924)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1908..1924)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1921..1943)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2096..2684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2176..2195)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2801..2825)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2925..3716,3717..3785))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3548..3567
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3786..3890)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3989..4011
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4003..4020
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4004..4020
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4644..4645,1..32))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aacttcgtat agcatacatt atacgaagtt atgatatcaa aaatgattac gccaagctgt
       61 aagtttaaac atgatcttac taactaacta ttctcattta aattttcaga gcttaaaaat
      121 ggctgaaatc actcacaacg atggatacgc taatgcctcc tatccaacga gtcttgccag
      181 tggttttttc acatgatgaa atcgttgttg gtcatttgaa aataacatca attactgcat
      241 cactctctcc ccagatccaa ggccccactc gggtttgctc agaaagacgc tcagtattgg
      301 gcggggaaca tgggaacaac gactttggta ctatttctgt tttgcctctt cgcgttccgc
      361 cccttcaaac cttcctataa aaaggatgag cacacaactg ggagtttaat gtagcttttg
      421 tgattgtgct tgtttcaaac acaaacgttt aaaaaaaaga gaccaaaaat ggggcccgtt
      481 tcaaagggag aggccgttat aaaagaattt atgcgtttca aggtccacat ggagggatcc
      541 atgaacggac acgagttcga gatcgaggga gagggagagg gacgtccata cgagggaacc
      601 caaaccgcca aggtgagttt tattatcgat tttcgggatt ttctacttgt aattaccttt
      661 tgtttttaat aagttgcttt tttccagctc aaggtcacca agggaggacc actcccattc
      721 tcctgggaca tcctctcccc acaattcatg tacggatccc gtgccttcac caagcaccca
      781 gccgacatcc cagactacta caagcaatcc ttcccagagg gattcaagtg ggagcgtgtc
      841 atgaacttcg aggacggagg agccgtcacc gtcacccaag gtaggttttc agtttcaatt
      901 tttgttgcaa ctacctcaac tctatgtttt cagacacctc cctcgaggac ggaaccctca
      961 tctacaaggt caagctccgt ggaaccaact tcccaccaga cggaccagtc atgcaaaaga
     1021 agaccatggg atgggaggcc tccaccgagc gactctaccc agaggacgga gtcctcaagg
     1081 gagacatcaa ggtaaatttt tcaagcttct aaattccaaa ataaaccatt ttaatttctt
     1141 aattccagat ggccctccgg ctcaaggacg gaggacgtta cctcgccgac ttcaagacca
     1201 cctacaaggc caagaagcca gtccaaatgc caggagccta caacgtcgac cgtaagctcg
     1261 acatcacctc ccacaacgag gactacaccg tcgtcgagca atacgagcgt tccgagggac
     1321 gtcactccac cggaggaatg gacgagctct acaagcccgg gtgatggtct ctgagctatg
     1381 caaaatcctt tcaagcattc ccttcttctc tatcactctt ctttcttttt gtcaaaaaat
     1441 tctctcgcta atttatttgc ttttttaatg ttattatttt atgacttttt atagtcactg
     1501 aaaagtttgc atctgagtga agtgaatgct atcaaaatgt gattctgata tccaggaaac
     1561 ggagaatctg tgcggcaatg tcttatcatt tgaaacgcgc cgtatcgatg ctgacatcat
     1621 gcggctcccg tgttagtcga gaagatgggt acgggccttt attcatccaa cactctacgg
     1681 cttcttcaag agctagtagg gcaccctgta gttggaaagg gattatttcg tatggcgagc
     1741 ccataccgtc tttcttgcgg aagcttaaca cgataggaag ttggatagtt tcgaacgatg
     1801 gttccatacg gaacgctgtc tcaaggatga gtctgacgga gtgtaactcg aaggctaggt
     1861 ggaggctcag tgatgataag tctgcgatgg tggatgcatg tgtcatggtc atagctgttt
     1921 cctgtgtgaa attgttatcc gctcagaggg cacaatccta ttccgcgcta tccgacaatc
     1981 tccaagacat taggtggagt tcagttcggc gtatggcata tgtcgctgga aagaacatgt
     2041 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2101 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2161 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2221 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2281 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2341 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2401 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2461 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2521 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2581 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2641 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2701 tttctacggg gtctgacgct ctattcaaca aagccgccgt cccgtcaagt cagcgtaaat
     2761 gggtaggggg cttcaaatcg tcctcgtgat accaattcgg agcctgcttt tttgtacaaa
     2821 cttgttgata atggcaattc aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2881 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2941 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3001 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3061 ccgcgagagc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3121 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3181 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3241 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3301 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3361 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3421 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3481 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3541 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3601 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3661 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3721 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3781 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3841 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3901 cgaaaagtgc cagatacctg aaacaaaacc catcgtacgg ccaaggaagt ctccaataac
     3961 tgtgatccac cacaagcgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     4021 catgcataat ccgcacgcat ctggaataag gaagtgccat tccgcctgac ctaagtgctt
     4081 ttcgagatcg ccaaaccgga gaacgatcag gagaatgatg agtcgccaca ggagtcggca
     4141 agatacgtgc gttatagatt tgctccaaac tttttgaaac tcaaaacggt cggagcaact
     4201 gtggaattca aagtaggaga catcccaatc catcatttcc gaatgatcga acgtcacttc
     4261 ttcaaagatc gccttctgaa gtgttttgac tttgaattcg gattctgtat tccgaattca
     4321 cgaaacaact gtgaacatat ctatgagttc cctcaactct ctcaacaact cagtgagtca
     4381 ttattctaaa aagtacaatt caaaagacta atctcttttt cagtggacga catgatcaac
     4441 aatccaaacg agactcgttc tgacagcttc tatttcgtcg ataacaaact cgtcatgcac
     4501 aacaaagccg actactcata tgatgcataa atcttcattg ttgagtttat cttgttgatt
     4561 tttgaataaa ttatcaactc tttacttttt aatgggttat gaaataaata aacattgaaa
     4621 actgataaac aacgttcatc tctat
//
