LOCUS       sequence-397617-        4663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gfp BioPart for PVT neurons. (1) Mark neurons or (2) cell-specific 
            expression of transgenes (N or C-terminal fusions, or untagged). 
            Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH82 - [PVTp | gfp | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1898..1914)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1898..1914)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1911..1933)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2086..2674)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2166..2185)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2791..2815)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2915..3706,3707..3775))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3538..3557
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3776..3880)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3979..4001
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3993..4010
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3994..4010
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4634..4663,1..4))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttatgatatc aaaaatgatt acgccaagct gtaagtttaa acatgatctt actaactaac
       61 tattctcatt taaattttca gagcttaaaa atggctgaaa tcactcacaa cgatggatac
      121 gctaatgcct cctatccaac gagtcttgcc agtggttttt tcacatgatg aaatcgttgt
      181 tggtcatttg aaaataacat caattactgc atcactctct ccccagatcc aaggccccac
      241 tcgggtttgc tcagaaagac gctcagtatt gggcggggaa catgggaaca acgactttgg
      301 tactatttct gttttgcctc ttcgcgttcc gccccttcaa accttcctat aaaaaggatg
      361 agcacacaac tgggagttta atgtagcttt tgtgattgtg cttgtttcaa acacaaacgt
      421 ttaaaaaaaa gagaccaaaa atggggccct ctaagggcga agaattgttc acgggagtag
      481 tcccaatctt agtcgagctt gacggaggtg agttttatta tcgattttcg ggattttcta
      541 cttgtaatta ccttttgttt ttaataagtt gcttttttcc agatgttaat ggccacaagt
      601 tttccgtgtc tggagaagga gaaggcgacg ctacctacgg taagctgacc ctgaagttca
      661 tttgcactac tggcaagtta ccagtaccat ggccgaccct ggtgacaaca ttttgctacg
      721 gggttcaatg cttctccaga tacccaggta ggttttcagt ttcaattttt gttgcaacta
      781 cctcaactct atgttttcag atcatatgaa aagacacgac ttttttaaat ccgccatgcc
      841 agagggttac gttcaagaac gtaccatttt ttttaaggac gatggtaact acaagacacg
      901 tgcggaggtt aaatttgaag gcgatactct cgtgaaccgt attgagctca aggtaaattt
      961 ttcaagcttc taaattccaa aataaaccat tttaatttct taattccagg gaatcgattt
     1021 caaggaagat ggaaacattt tgggccacaa gcttgaatat aattataata gtcataatgt
     1081 ctacattatg gccgacaaac aaaaaaacgg tattaaggtt aacttcaaga ttcgccacaa
     1141 tatcgaagat ggatctgtcc aacttgctga ccactaccaa caaaatactc caattggaga
     1201 tggaccggtt ttgcttccag ataaccacta cctttccact cagtctgccc tctctaagga
     1261 tccgaatgaa aaacgtgacc atatggttct tttggagttc gttaccgcag caggaattac
     1321 tcacggaatg gatgaactgt acaagcccgg gtgatggtct ctgagctatg caaaatcctt
     1381 tcaagcattc ccttcttctc tatcactctt ctttcttttt gtcaaaaaat tctctcgcta
     1441 atttatttgc ttttttaatg ttattatttt atgacttttt atagtcactg aaaagtttgc
     1501 atctgagtga agtgaatgct atcaaaatgt gattctgata tccaggaaac ggagaatctg
     1561 tgcggcaatg tcttatcatt tgaaacgcgc cgtatcgatg ctgacatcat gcggctcccg
     1621 tgttagtcga gaagatgggt acgggccttt attcatccaa cactctacgg cttcttcaag
     1681 agctagtagg gcaccctgta gttggaaagg gattatttcg tatggcgagc ccataccgtc
     1741 tttcttgcgg aagcttaaca cgataggaag ttggatagtt tcgaacgatg gttccatacg
     1801 gaacgctgtc tcaaggatga gtctgacgga gtgtaactcg aaggctaggt ggaggctcag
     1861 tgatgataag tctgcgatgg tggatgcatg tgtcatggtc atagctgttt cctgtgtgaa
     1921 attgttatcc gctcagaggg cacaatccta ttccgcgcta tccgacaatc tccaagacat
     1981 taggtggagt tcagttcggc gtatggcata tgtcgctgga aagaacatgt gagcaaaagg
     2041 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2101 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2161 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2221 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2281 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2341 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2401 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2461 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2521 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2581 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2641 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2701 gtctgacgct ctattcaaca aagccgccgt cccgtcaagt cagcgtaaat gggtaggggg
     2761 cttcaaatcg tcctcgtgat accaattcgg agcctgcttt tttgtacaaa cttgttgata
     2821 atggcaattc aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2881 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     2941 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3001 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagagc
     3061 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3121 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     3181 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     3241 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     3301 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     3361 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     3421 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     3481 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     3541 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3601 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3661 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3721 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3781 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     3841 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     3901 cagatacctg aaacaaaacc catcgtacgg ccaaggaagt ctccaataac tgtgatccac
     3961 cacaagcgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt catgcataat
     4021 ccgcacgcat ctggaataag gaagtgccat tccgcctgac ctaagtgctt ttcgagatcg
     4081 ccaaaccgga gaacgatcag gagaatgatg agtcgccaca ggagtcggca agatacgtgc
     4141 gttatagatt tgctccaaac tttttgaaac tcaaaacggt cggagcaact gtggaattca
     4201 aagtaggaga catcccaatc catcatttcc gaatgatcga acgtcacttc ttcaaagatc
     4261 gccttctgaa gtgttttgac tttgaattcg gattctgtat tccgaattca cgaaacaact
     4321 gtgaacatat ctatgagttc cctcaactct ctcaacaact cagtgagtca ttattctaaa
     4381 aagtacaatt caaaagacta atctcttttt cagtggacga catgatcaac aatccaaacg
     4441 agactcgttc tgacagcttc tatttcgtcg ataacaaact cgtcatgcac aacaaagccg
     4501 actactcata tgatgcataa atcttcattg ttgagtttat cttgttgatt tttgaataaa
     4561 ttatcaactc tttacttttt aatgggttat gaaataaata aacattgaaa actgataaac
     4621 aacgttcatc tctataactt cgtatagcat acattatacg aag
//
