LOCUS       sequence-397618-        4663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gfp BioPart for AWC neurons. (1) Mark neurons or (2) cell-specific 
            expression of transgenes (N or C-terminal fusions, or untagged). 
            Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH86 - [AWCp | gfp | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1920..1936)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1920..1936)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1933..1955)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2108..2696)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2188..2207)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2813..2837)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2937..3728,3729..3797))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3560..3579
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3798..3902)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4001..4023
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4015..4032
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4016..4032
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4656..4663,1..26))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gtatagcata cattatacga agttatgata tcaaaaatga ttacgccaag ctgtaagttt
       61 aaacatgatc ttactaacta actattctca tttaaatttt cagagcttaa aaatggctga
      121 aatcactcac aacgatggat acgctaatgc cactccaaaa aattgatgtt tcgcgattat
      181 ttcaaggcaa ctatacaaaa tggtaatttt aatacctttc tcgatttaat ttcgtcataa
      241 ctttgcttcg tcataaaaat aagcttactc atcccccatt ctatacaaac ttattttcaa
      301 ttgtttagct cttattcaac taaatcctat tccccctatt ctccttaaca ttccttaaat
      361 atcaaactct aaaacaaatg atctattgac gtctgatcta ctgggtataa aaacccctta
      421 attcctacct ataacctctc tttatcaaaa aagagaccaa aaatggggcc ctctaagggc
      481 gaagaattgt tcacgggagt agtcccaatc ttagtcgagc ttgacggagg tgagttttat
      541 tatcgatttt cgggattttc tacttgtaat taccttttgt ttttaataag ttgctttttt
      601 ccagatgtta atggccacaa gttttccgtg tctggagaag gagaaggcga cgctacctac
      661 ggtaagctga ccctgaagtt catttgcact actggcaagt taccagtacc atggccgacc
      721 ctggtgacaa cattttgcta cggggttcaa tgcttctcca gatacccagg taggttttca
      781 gtttcaattt ttgttgcaac tacctcaact ctatgttttc agatcatatg aaaagacacg
      841 acttttttaa atccgccatg ccagagggtt acgttcaaga acgtaccatt ttttttaagg
      901 acgatggtaa ctacaagaca cgtgcggagg ttaaatttga aggcgatact ctcgtgaacc
      961 gtattgagct caaggtaaat ttttcaagct tctaaattcc aaaataaacc attttaattt
     1021 cttaattcca gggaatcgat ttcaaggaag atggaaacat tttgggccac aagcttgaat
     1081 ataattataa tagtcataat gtctacatta tggccgacaa acaaaaaaac ggtattaagg
     1141 ttaacttcaa gattcgccac aatatcgaag atggatctgt ccaacttgct gaccactacc
     1201 aacaaaatac tccaattgga gatggaccgg ttttgcttcc agataaccac tacctttcca
     1261 ctcagtctgc cctctctaag gatccgaatg aaaaacgtga ccatatggtt cttttggagt
     1321 tcgttaccgc agcaggaatt actcacggaa tggatgaact gtacaagccc gggtgatggt
     1381 ctctgagcta tgcaaaatcc tttcaagcat tcccttcttc tctatcactc ttctttcttt
     1441 ttgtcaaaaa attctctcgc taatttattt gcttttttaa tgttattatt ttatgacttt
     1501 ttatagtcac tgaaaagttt gcatctgagt gaagtgaatg ctatcaaaat gtgattctga
     1561 tatccaggaa acggagaatc tgtgcggcaa tgtcttatca tttgaaacgc gccgtatcga
     1621 tgctgacatc atgcggctcc cgtgttagtc gagaagatgg gtacgggcct ttattcatcc
     1681 aacactctac ggcttcttca agagctagta gggcaccctg tagttggaaa gggattattt
     1741 cgtatggcga gcccataccg tctttcttgc ggaagcttaa cacgatagga agttggatag
     1801 tttcgaacga tggttccata cggaacgctg tctcaaggat gagtctgacg gagtgtaact
     1861 cgaaggctag gtggaggctc agtgatgata agtctgcgat ggtggatgca tgtgtcatgg
     1921 tcatagctgt ttcctgtgtg aaattgttat ccgctcagag ggcacaatcc tattccgcgc
     1981 tatccgacaa tctccaagac attaggtgga gttcagttcg gcgtatggca tatgtcgctg
     2041 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2101 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2161 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2221 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2281 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2341 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2401 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2461 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2521 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     2581 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2641 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2701 atcctttgat cttttctacg gggtctgacg ctctattcaa caaagccgcc gtcccgtcaa
     2761 gtcagcgtaa atgggtaggg ggcttcaaat cgtcctcgtg ataccaattc ggagcctgct
     2821 tttttgtaca aacttgttga taatggcaat tcaaggatct tcacctagat ccttttaaat
     2881 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2941 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3001 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3061 gctgcaatga taccgcgaga gccacgctca ccggctccag atttatcagc aataaaccag
     3121 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3181 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3241 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3301 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3361 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3421 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     3481 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3541 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3601 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3661 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3721 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3781 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3841 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3901 cgcacatttc cccgaaaagt gccagatacc tgaaacaaaa cccatcgtac ggccaaggaa
     3961 gtctccaata actgtgatcc accacaagcg ccagggtttt cccagtcacg acgttgtaaa
     4021 acgacggcca gtcatgcata atccgcacgc atctggaata aggaagtgcc attccgcctg
     4081 acctaagtgc ttttcgagat cgccaaaccg gagaacgatc aggagaatga tgagtcgcca
     4141 caggagtcgg caagatacgt gcgttataga tttgctccaa actttttgaa actcaaaacg
     4201 gtcggagcaa ctgtggaatt caaagtagga gacatcccaa tccatcattt ccgaatgatc
     4261 gaacgtcact tcttcaaaga tcgccttctg aagtgttttg actttgaatt cggattctgt
     4321 attccgaatt cacgaaacaa ctgtgaacat atctatgagt tccctcaact ctctcaacaa
     4381 ctcagtgagt cattattcta aaaagtacaa ttcaaaagac taatctcttt ttcagtggac
     4441 gacatgatca acaatccaaa cgagactcgt tctgacagct tctatttcgt cgataacaaa
     4501 ctcgtcatgc acaacaaagc cgactactca tatgatgcat aaatcttcat tgttgagttt
     4561 atcttgttga tttttgaata aattatcaac tctttacttt ttaatgggtt atgaaataaa
     4621 taaacattga aaactgataa acaacgttca tctctataac ttc
//
