LOCUS       sequence-39763-e         591 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the eGFP reporter gene was not detected in the brain 
            and eye..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1433
FEATURES             Location/Qualifiers
     source          1..591
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..21
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt aattccgatc atattcaata
       61 acccttaatg aattcgagct ctcttcaagc cttctccagt atgatctgat tggacaaaca
      121 gtatctagtg agacaattaa atctaacaca ggtaagaatt tgaccaaagg atggaaataa
      181 tccctcagga tgactccagt gcacattttc aaaatatcta tttctttttc cttcgcctta
      241 gccattctca gctaagcatg ctgttttgca gtgatgagta gctttcctta aaactgattt
      301 tgcaaccagg tgcatataaa gatttgtgta atcttggaat aagtagtttt ttttatgtat
      361 atgatataaa gaaccctact gaaaacaata agttgatgtg tctcttcgct ctatcatctg
      421 ctgatcaagt agctttctca tatactgaaa tgtacctttg cttgttttct tccagtcaat
      481 gttaatttaa aagttgtcaa gatttatatt caactgcatt ctttttgcag actgtcagca
      541 gtaataatac tgatgtatgt gtcttccaac aggtgaatac gttataatga c
//
