LOCUS       sequence-397638-       11588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac Vector expressing KEIMA-RAMP4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11588)
  AUTHORS   Hoyer MJ, Capitanio C, Smith IR, Paoli JC, Bieber A, Jiang Y, Paulo 
            JA, Gonzalez-Lozano MA, Baumeister W, Wilfling F, Schulman BA, 
            Harper JW
  TITLE     Combinatorial selective ER-phagy remodels the ER during 
            neurogenesis.
  JOURNAL   Nat Cell Biol. 2024 Mar;26(3):378-392. doi: 
            10.1038/s41556-024-01356-4. Epub 2024 Mar 1.
  PUBMED    38429475
REFERENCE   2  (bases 1 to 11588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-024-01356-4"; journalName: "Nat Cell Biol"; date: 
            "2024-03"; volume: "26"; issue: "3"; pages: "378-392"
COMMENT     Sequence Label: pAC150-KEIMA-RAMP4
FEATURES             Location/Qualifiers
     source          1..11588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   299..333
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    667..700
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        850..1229
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1230..1433
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1384..1404
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1430..1454
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2618..3613
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    3649..3783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3686..3705)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3740..3759
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3812..3845
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4487..5665
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4718..5656
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5613..5633
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    5726..5750
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(5759..5761,5762..5764,5765..6424)
                     /codon_start=1
                     /product="humanized monomeric Keima-Red fluorescent 
                     protein"
                     /label=hmKeima-Red
                     /note="mammalian codon-optimized"
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
                     "
     protein_bind    complement(6654..6678)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6731..6772
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6810..6827)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6816..7040
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7860..7922
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7960..7979)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7961..7979)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7986..8003)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7986..8002)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7995..8017)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             8140..8442
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8321..8340
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             8791..9585
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8791..9585
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8845..8864)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9455..9474
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(9835..10626,10627..10695))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10458..10477
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10819..11407
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11308..11327
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11561..11578
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc ccttttaacc
      301 ctagaaagat agtctgcgta aaattgacgc atgcattctt gaaatattgc tctctctttc
      361 taaatagcgc gaatccgtcg ctgtgcattt aggacatctc agtcgccgct tggagctccc
      421 gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt gaactataac gaccgcgtga
      481 gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca tacgataatt
      541 atattgttat ttcatgttct acttacgtga taacttatta tatatatatt ttcttgttat
      601 agatatcaac tagaatgcta gacctttcgt cttcaagaat tccgatcata ttcaataacc
      661 cttaatataa cttcgtataa tgtatgctat acgaagttat taggtctgaa gaggagttta
      721 cgtccagcca agcttaggat cccgggtacc gatggccggg cctcgaggag cttggcccat
      781 tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac
      841 cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
      901 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      961 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     1021 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     1081 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     1141 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1201 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     1261 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     1321 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     1381 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     1441 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
     1501 gggaccgatc cagcctcccc ggctagccgc cgccaccatg aaaaagcctg aactcaccgc
     1561 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     1621 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     1681 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     1741 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac
     1801 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     1861 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
     1921 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     1981 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     2041 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     2101 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     2161 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     2221 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     2281 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
     2341 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     2401 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     2461 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtcg cgtctgcgtt
     2521 cgaccaggct gcgcgttctc gcggccatag caaccgacgt acggcgttgc gccctcgccg
     2581 gcagcaagaa gccacggaag tccgcccgga gcagaaaatg cccacgctac tgcgggttta
     2641 tatagacggt ccccacggga tggggaaaac caccaccacg caactgctgg tggccctggg
     2701 ttcgcgcgac gatatcgtct acgtacccga gccgatgact tactggcggg tgctgggggc
     2761 ttccgagaca atcgcgaaca tctacaccac acaacaccgc ctcgaccagg gtgagatatc
     2821 ggccggggac gcggcggtgg taatgacaag cgcccagata acaatgggca tgccttatgc
     2881 cgtgaccgac gccgttctgg ctcctcatat cgggggggag gctgggagct cacatgcccc
     2941 gcccccggcc ctcaccctca tcttcgaccg ccatcccatc gccgccctcc tgtgctaccc
     3001 ggccgcgcgg taccttatgg gcagcatgac cccccaggcc gtgctggcgt tcgtggccct
     3061 catcccgccg accttgcccg gcaccaacat cgtgcttggg gcccttccgg aggacagaca
     3121 catcgaccgc ctggccaaac gccagcgccc cggcgagcgg ctggacctgg ctatgctggc
     3181 tgcgattcgc cgcgtttacg ggctacttgc caatacggtg cggtatctgc agtgcggcgg
     3241 gtcgtggcgg gaggactggg gacagctttc ggggacggcc gtgccgcccc agggtgccga
     3301 gccccagagc aacgcgggcc cacgacccca tatcggggac acgttattta ccctgtttcg
     3361 ggcccccgag ttgctggccc ccaacggcga cctgtataac gtgtttgcct gggccttgga
     3421 cgtcttggcc aaacgcctcc gttccatgca cgtctttatc ctggattacg accaatcgcc
     3481 cgccggctgc cgggacgccc tgctgcaact tacctccggg atggtccaga cccacgtcac
     3541 cacccccggc tccataccga cgatatgcga cctggcgcgc acgtttgccc gggagatggg
     3601 ggaggctaac tgaagcttcc cgggggtacc gaattcgtcg acagatctaa cttgtttatt
     3661 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3721 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     3781 atcatcaagc ttaggatccg gaacccttaa tataacttcg tataatgtat gctatacgaa
     3841 gttattaggt ccctcgaaga ggttcactgg cgcgttggat ccccgggtac cgagttggga
     3901 gctcacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct
     3961 agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc
     4021 gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct
     4081 ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaagc
     4141 tttaggctga aagagagatt tagaatgaca gtctagtggg agctcacggg gacagccccc
     4201 ccccaaagcc cccagggatg taattacgtc cctcccccgc tagggggcag cagcgagccg
     4261 cccggggctc cgctccggtc cggcgctccc cccgcatccc cgagccggca gcgtgcgggg
     4321 acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct tctcgctgct
     4381 ctttgagcct gcagacacct ggggggatac ggggaaaaag ctttaggctg aaagagagat
     4441 ttagaatgac agaactcgat ttcattgcag actggcgcgc ccgtgaggct ccggtgcccg
     4501 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     4561 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     4621 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     4681 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     4741 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     4801 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     4861 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     4921 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     4981 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     5041 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     5101 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     5161 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     5221 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     5281 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     5341 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     5401 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     5461 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     5521 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     5581 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     5641 ttttcttcca tttcaggtgt cgtgaagaag cttggtaccg agctcggatc cactagtcca
     5701 gtgtggtgga attctgcaga tatcaacaag tttgtacaaa aaagcaggct gcggtaccat
     5761 ggtgagcgtg atcgccaagc agatgaccta caaggtgtac atgagcggca ccgtgaacgg
     5821 ccactacttc gaggtggagg gcgacggcaa gggcaagccc tacgagggcg agcagaccgt
     5881 gaagctgacc gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgagccccca
     5941 gctccagtac ggcagcatcc ccttcaccaa gtaccccgag gacatccccg actacttcaa
     6001 gcagagcttc cccgagggct acacctggga gcgcagcatg aacttcgagg acggcgccgt
     6061 gtgcaccgtg agcaacgaca gcagcatcca gggcaactgc ttcatctaca acgtgaagat
     6121 cagcggcgag aacttccccc ccaacggccc cgtgatgcag aagaagaccc agggctggga
     6181 gcccagcacc gagcgcctgt tcgcccgcga cggaatgctg atcggcaacg actacatggc
     6241 cctgaagctg gagggcggcg gccactacct gtgcgagttc aagagcacct acaaggccaa
     6301 gaagcccgtg aggatgcccg gccgccacga gatcgaccgc aagctggacg tgaccagcca
     6361 caaccgcgac tacaccagcg tggagcagtg cgagatcgcc atcgcccgcc actccctgct
     6421 gggcggaggc tcaggaggct caggaggctc aatggtcgcc aagcaaagga tccgtatggc
     6481 caacgagaag cacagcaaga acatcaccca gcgcggcaac gtcgccaaga cctcgagaaa
     6541 tgcccccgaa gagaaggcgt ctgtaggacc ctggttattg gctctcttca tttttgttgt
     6601 ctgtggttct gcaattttcc agattattca aagtatcagg atgggcatgt gagacccagc
     6661 tttcttgtac aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc
     6721 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg
     6781 ttaggggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     6841 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     6901 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     6961 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     7021 ggatgcggtg ggctctatgg cggcgcgcct ggatccccgg gtaccgagtt gggagctcac
     7081 ggggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg
     7141 cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg
     7201 gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac
     7261 gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa aagctttagg
     7321 ctgaaagaga gatttagaat gacagtctag tgggagctca cggggacagc ccccccccaa
     7381 agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg
     7441 gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc
     7501 cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga
     7561 gcctgcagac acctgggggg atacggggaa aaagctttag gctgaaagag agatttagaa
     7621 tgacagaact cgatttcatt gcagactggc cggccactag tacgcgccgg ctcgacatac
     7681 tagttaaaag ttttgttact ttatagaaga aattttgagt ttttgttttt ttttaataaa
     7741 taaataaaca taaataaatt gtttgttgaa tttattatta gtatgtaagt gtaaatataa
     7801 taaaacttaa tatctattca aattaataaa taaacctcga tatacagacc gataaaacac
     7861 atgcgtcaat tttacgcatg attatcttta acgtacgtca caatatgatt atctttctag
     7921 ggttaaaagg gcgaattcgc ggccgctaaa ttcaattcgc cctatagtga gtcgtattac
     7981 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     8041 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     8101 gatcgccctt cccaacagtt gcgcagccta tacgtacggc agtttaaggt ttacacctat
     8161 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccg
     8221 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
     8281 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     8341 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     8401 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg catgagatta
     8461 tcaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc
     8521 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag
     8581 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg
     8641 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
     8701 aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagctct
     8761 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     8821 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     8881 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     8941 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     9001 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     9061 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     9121 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     9181 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     9241 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     9301 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     9361 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     9421 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     9481 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     9541 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaattat taacgcttac
     9601 aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatcagg
     9661 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     9721 aaatatgtat ccgctcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9781 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9841 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9901 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9961 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    10021 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10081 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10141 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10201 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10261 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10321 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10381 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10441 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10501 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10561 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10621 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10681 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10741 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    10801 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    10861 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    10921 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    10981 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    11041 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    11101 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    11161 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    11221 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    11281 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    11341 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    11401 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    11461 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    11521 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    11581 agcggaag
//
