LOCUS       sequence-397639-       11227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for wild type, myc-tagged EFNB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11227)
  AUTHORS   Narayanan KK, Amaya M, Tsang N, Yin R, Jays A, Broder CC, Shukla D, 
            Procko E
  TITLE     Sequence basis for selectivity of ephrin-B2 ligand for Eph receptors
            and pathogenic henipavirus G glycoproteins.
  JOURNAL   J Virol. 2023 Nov 30;97(11):e0062123. doi: 10.1128/jvi.00621-23. 
            Epub 2023 Oct 16.
  PUBMED    37931130
REFERENCE   2  (bases 1 to 11227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2023-11-30"; volume: "97(11):e0062123. doi"; pages: " 
            10.1128/jvi.00621-23. Epub 2023 Oct 16"
COMMENT     Sequence Label: pCEP4-myc-EFNB2 (WT)
FEATURES             Location/Qualifiers
     source          1..11227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    14..148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(38..57)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     92..111
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(291..313)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      903..2692
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(2994..4919)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGXGGAGXGGXGGXGXGGGGGXGGGGGGGGGGXG
                     GGGGGGGGGGGGGGGGGGXGGXGXGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(5354..5372)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5440..5544
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5545..5613,5614..6405)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5763..5782)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6576..7164
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7065..7084
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7595..7740
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             7783..8820
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(8818..8837)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8862..8910
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        9564..9943
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9944..10147
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10098..10118
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     10144..10168
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             10250..10279
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gccggcaagg ccggatccag acatgataag atacattgat gagtttggac aaaccacaac
       61 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      121 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
      181 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
      241 ggctgattat gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
      301 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
      361 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgactcta
      421 gaggatcgat ccccgccccg gacgaactaa acctgactac gacakcycyg smcswwctwm
      481 rcskgrcwrc gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg
      541 gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac
      601 aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac
      661 tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct
      721 ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg
      781 ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc
      841 gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc
      901 taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta accctaattc
      961 aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc
     1021 ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca
     1081 ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag
     1141 cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata
     1201 gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca
     1261 ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca
     1321 ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct
     1381 gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca
     1441 tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt
     1501 tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt
     1561 gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca
     1621 cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca
     1681 ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg
     1741 ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc
     1801 ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag
     1861 gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt
     1921 gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac
     1981 ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta
     2041 tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     2101 tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta
     2161 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     2221 tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta
     2281 tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     2341 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     2401 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     2461 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     2521 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
     2581 catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta tcctttgcat
     2641 atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc tgctggttgc
     2701 tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc tgattgctca
     2761 ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg agctgagtga
     2821 cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg gtgacaagac
     2881 agatggccag aaatacacca acagcacgca tgatgtctac tggggattta ttctttagtg
     2941 cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt acatcactcc
     3001 tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc gtcatcaccc
     3061 tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac tccatcgtca
     3121 aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat aacaaggtcc
     3181 ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac catgaaataa
     3241 cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc cattccaaag
     3301 gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt tcctcgcctt
     3361 aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac ccaagttcct
     3421 tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc ttctgcaatg
     3481 ttctcaaatt tcgggttgga acctccttga ccacgatgct ttccaaacca ccctcctttt
     3541 ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc tcctgggtca
     3601 tctgcggggc cctgctctat cgctcccggg ggcacgtcag gctcaccatc tgggccacct
     3661 tcttggtggt attcaaaata atcggcttcc cctacagggt ggaaaaatgg ccttctacct
     3721 ggagggggcc tgcgcggtgg agacccggat gatgatgact gactactggg actcctgggc
     3781 ctcttttctc cacgtccacg acctctcccc ctggctcttt cacgacttcc ccccctggct
     3841 ctttcacgtc ctctaccccg gcggcctcca ctacctcctc gaccccggcc tccactacct
     3901 cctcgacccc ggcctccact gcctcctcga ccccggcctc cacctcctgc tcctgcccct
     3961 cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctsctcc tscccctcct
     4021 scccctcctc ctcctccccc tcctccccct cctcctcctc cccctcctcc ccctcctcct
     4081 sctcctcccc ctcctccccc tcctcctcct cctscccctc ctccccctcc tsctcctscc
     4141 cctcctsccc ctcctsctcc tgcccctcct scccctcctg ctcctgcccc tcctgctcct
     4201 gcccctcctg ctcctgcccc tcctgctcct gcccctcctg cccctcctgc ccctcctcct
     4261 gctcctgccc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc tcctgcccct
     4321 cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc
     4381 cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgcccctcct
     4441 cctgctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct
     4501 cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc
     4561 cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct
     4621 gctcctgccc ctcccgctcc tgctcctgct cctgttccac cgtgggtccc tttgcagcca
     4681 atgcaacttg gacgtttttg gggtctccgg acaccatctc tatgtcttgg ccctgatcct
     4741 gagccgcccg gggctcctgg tcttccgcct cctcgtcctc gtcctcttcc ccgtcctcgt
     4801 ccatggttat caccccctct tctttgaggt ccactgccgc cggagccttc tggtccagat
     4861 gtgtctccct tctctcctag gccatttcca ggtcctgtac ctggcccctc gtcagacatg
     4921 attcacacta aaagagatca atagacatct ttattagacg acgctcagtg aatacaggga
     4981 gtgcagactc ctgccccctc caacagcccc cccaccctca tccccttcat ggtcgctgtc
     5041 agacagatcc aggtctgaaa attccccatc ctccgaacca tcctcgtcct catcaccaat
     5101 tactcgcagc ccggaaaact cccgctgaac atcctcaaga tttgcgtcct gagcctcaag
     5161 ccaggcctca aattcctcgt cccccttttt gctggacggt agggatgggg attctcggga
     5221 cccctcctct tcctcttcaa ggtcaccaga cagagatgct actggggcaa cggaagaaaa
     5281 gctgggtgcg gcctgtgagg atcagcttat cgatgataag ctgtcaaaca tgagaattct
     5341 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     5401 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5461 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5521 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5581 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5641 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5701 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5761 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     5821 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5881 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5941 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6001 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6061 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     6121 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6181 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6241 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6301 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6361 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6421 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6481 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6541 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6601 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6661 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6721 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6781 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6841 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6901 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6961 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7021 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7081 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7141 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7201 ttttgctggc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc
     7261 tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc
     7321 cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggcccc gagatgcgcc
     7381 gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg
     7441 cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag
     7501 cgaggtgccg ccctgcttca tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc
     7561 ggaagaaata tatttgcatg tctttagttc tatgatgaca caaaccccgc ccagcgtctt
     7621 gtcattggcg aattcgaaca cgcagatgca gtcggggcgg cgcggtccga ggtccacttc
     7681 gcatattaag gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg acccgcttaa
     7741 cagcgtcaac agcgtgccgc agatcccggg gggcaatgag atatgaaaaa gcctgaactc
     7801 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     7861 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     7921 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     7981 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc
     8041 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     8101 gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
     8161 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     8221 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     8281 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     8341 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     8401 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     8461 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     8521 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg
     8581 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     8641 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     8701 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     8761 gatagtggaa accgacgccc cagcactcgt ccggatcggg agatggggga ggctaactga
     8821 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     8881 taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     8941 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     9001 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     9061 caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca tggggaatgg
     9121 tttatggttc gtgggggtta ttattttggg cgttgcgtgg ggtcaggtcc acgactggac
     9181 tgagcagaca gacccatggt ttttggatgg cctgggcatg gaccgcatgt actggcgcga
     9241 cacgaacacc gggcgtctgt ggctgccaaa cacccccgac ccccaaaaac caccgcgcgg
     9301 atttctggcg tgccaagcta gtcgaccaat tctcatgttt gacagcttat catcgcagat
     9361 ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg taggtatgga agatctatac
     9421 attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat
     9481 tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc
     9541 atgtccaata tgaccgccat gttgacattg attattgact agttattaat agtaatcaat
     9601 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     9661 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     9721 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     9781 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt
     9841 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc
     9901 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     9961 gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
    10021 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
    10081 taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
    10141 cagagctcgt ttagtgaacc gtcagatctc tagaagctgg gtaccagctg ctagcgccac
    10201 catgaagacc atcatcgccc tgagctacat cttctgcctg gtgttcgccg agcaaaagct
    10261 gatctcagag gaggacctgg gcagccctgg cggtgccatc gtgttggagc ccatatattg
    10321 gaatagctcc aattcaaaat ttctgccagg acagggactc gttctctatc ctcaaatcgg
    10381 cgacaaattg gatattatat gccctaaggt tgactctaag actgttgggc agtatgagta
    10441 ttataaagtg tatatggtag ataaggatca ggcggatcgc tgtactatca aaaaagagaa
    10501 tacgcctctt ctgaactgtg cgaaacccga tcaagatatt aaatttacaa ttaagtttca
    10561 agaattttca cctaatttgt ggggtcttga gttccagaag aacaaagact attacatcat
    10621 tagcacctcc aacggaagcc ttgagggtct ggataatcag gaaggtggag tttgtcaaac
    10681 tcgcgccatg aaaattctga tgaaggtagg acaggacgcg agctcagcgg gatcaacgcg
    10741 gaataaagat ccgacacgga ggccggagct cgaagctggg actaatggta gatcctctac
    10801 aacttcaccc tttgtaaaac caaacccggg cagtagtacg gacggcaaca gcgccgggca
    10861 ctcagggaat aatatactgg ggtccgaggt tgcactgttc gctgggatag catccggttg
    10921 tataatcttt attgtcataa ttattacatt ggtagtattg ctccttaagt atcgccgacg
    10981 ccatcggaaa cactccccgc aacacacaac aacactttcc ctgtccacat tggctacccc
    11041 gaagaggagt ggtaataata acggttctga acccagtgat atcataatcc cgttgagaac
    11101 tgccgacagc gtgttctgcc cgcattacga gaaagtctct ggtgactatg ggcatcctgt
    11161 ttatatagta caagagatgc ctccccagag tccagcgaac atttattaca aggtatgata
    11221 actcgag
//
