LOCUS       sequence-397644-        9827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For lentiviral transduction of FAM134C-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Hoyer MJ, Capitanio C, Smith IR, Paoli JC, Bieber A, Jiang Y, Paulo 
            JA, Gonzalez-Lozano MA, Baumeister W, Wilfling F, Schulman BA, 
            Harper JW
  TITLE     Combinatorial selective ER-phagy remodels the ER during 
            neurogenesis.
  JOURNAL   Nat Cell Biol. 2024 Mar;26(3):378-392. doi: 
            10.1038/s41556-024-01356-4. Epub 2024 Mar 1.
  PUBMED    38429475
REFERENCE   2  (bases 1 to 9827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-024-01356-4"; journalName: "Nat Cell Biol"; date: 
            "2024-03"; volume: "26"; issue: "3"; pages: "378-392"
COMMENT     Sequence Label: pHAGE-FAM134C-GFP
FEATURES             Location/Qualifiers
     source          1..9827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2731..2755
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     2813..2832
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2813..2831
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2861..2885
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4284..4308)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4319..4321,4322..4324,4325..5038)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4319..4321,4322..4324,4325..5035)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4364..4385)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4625..4644)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4972..4993
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5107..5606
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5142..5165)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5539..5558
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5630..6028
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6070..6191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6107..6126)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    6217..6805
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6270..6290)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6688..6699)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6880..7113
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7232..7250)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7318..7422
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7423..7491,7492..8283)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7641..7660)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8454..9042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8943..8962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9196..9213
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9453..9474
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9489..9494,9495..9512,9513..9519)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9527..9543
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9532..9554
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9551..9567
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9551..9567
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccga tctaatacga
     2821 ctcactatag ggagacccaa gctggctagt taagctatca acaagtttgt acaaaaaagt
     2881 tggcatggct gaggccgaag gggttcccac gaccccaggc ccggcttcgg ggtcgacttt
     2941 caggggccgc cgagatgtgt caggctcctg ggagcgggac cagcaggttg aggcggcgca
     3001 gcgggccctg gtggaggtgc tggggcctta cgagcctctg ctgagtcggg tgcaggcagc
     3061 cctggtgtgg gagcggccag ctaggagcgc tctgtggtgc ctggggctga acgcggcttt
     3121 ctggtttttt gccctgacat ctcttcgtct tgtgttttta cttgcatttg gcttgatgat
     3181 cattgtgtgt attgatcaat ggaagaacaa aatctggcct gaaataaaag tgccaagacc
     3241 cgacgcatta gacaatgaga gctggggctt tgtgcaccct cggttgctca gcgtgcccga
     3301 gctctgccac catgtagctg aagtctgggt tagtgggacc attttcataa ggaatgtttt
     3361 gcttttcaaa aagcaaaacc caggcaagtt ctgcttgctg agctgtggga tactgacctt
     3421 tttggctgtc ttgggccgct acgtccctgg gcttctgctg tcctacttga tgcttgtcac
     3481 tgtcatgatg tggccccttg ctgtgtacca ccgactgtgg gatcgagcat atgtgcggct
     3541 gaagccagct ctgcagcggc tagacttcag tgtccgtggc tacatgatgt ccaagcagag
     3601 agagagacaa ttacgccgca gagctctcca cccagaacga gccatggaca accacagtga
     3661 cagcgaagag gagcttgctg ccttctgtcc tcagctggac gattctactg ttgccaggga
     3721 attggccatc acagactctg agcactcaga cgctgaagtc tcctgtacag acaatggcac
     3781 attcaatctt tcaaggggcc aaacacctct aacggaaggc tctgaagacc tagatggtca
     3841 cagtgatcca gaggaatcct ttgccagaga ccttccagac ttcccttcca ttaatatgga
     3901 tcctgctggc ctggatgatg aggacgacac tagcattggc atgcccagct tgatgtaccg
     3961 ttctccgcca ggggctgagg agccccaggc cccacctgcc agccgggacg aggctgcgct
     4021 gccggagctc ctgcttggtg ctcttcctgt aggatccaac ctcaccagca accttgccag
     4081 cctggtctcc cagggtatga ttcagctggc cttgtcaggg gcctcccaac caggcccttc
     4141 tggagcacct gcccagagag caacgagagg cttcctccgg tcccccagtt cagacctgga
     4201 cactgatgct gagggggatg actttgaact tctggaccag tcggagctga gtcagctgga
     4261 ccctgccagt tctaggagcc actgcccaac tttcttgtac aaagtggttg atctagagat
     4321 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     4381 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     4441 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     4501 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     4561 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     4621 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     4681 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     4741 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     4801 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     4861 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     4921 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     4981 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaacg
     5041 cgttacgttg gaaacggggg aggctaactg aaacacggaa ggagctcgag gttaacgaat
     5101 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     5161 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     5221 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     5281 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     5341 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     5401 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     5461 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     5521 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     5581 cctcttcctc atctccgggc ctttcgacct gcagcccatt aattaaacca tggccaagcc
     5641 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc
     5701 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
     5761 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac
     5821 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
     5881 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat
     5941 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
     6001 gccctctggt tatgtgtggg agggctaagc acttcgtggc cgaggagcag gactgacact
     6061 cgacctcgaa acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6121 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6181 aatgtatctt atcatgtcta tgcatcgata gatcctaatc aacctctgga ttacaaaatt
     6241 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6301 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6361 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6421 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6481 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6541 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6601 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     6661 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6721 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6781 atctcccttt gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag
     6841 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     6901 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     6961 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7021 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7081 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     7141 agtatttata acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg
     7201 aaagggctag atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     7261 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     7321 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7381 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7441 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7501 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7561 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7621 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     7681 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7741 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     7801 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     7861 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     7921 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     7981 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     8041 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8101 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8161 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8221 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8281 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     8341 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8401 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8461 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8521 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8581 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     8641 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8701 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8761 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8821 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     8881 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8941 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9001 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9061 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9121 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9181 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     9241 ccgcctctcc ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt
     9301 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     9361 gaggcttttt tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc
     9421 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     9481 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     9541 caatttcaca caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt
     9601 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     9661 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
     9721 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
     9781 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt
//
