LOCUS       sequence-397648-        6034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV 
            production vector for astrocytes specific expression using the 
            promoter GFAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Lu X, Wang Y, Liu Z, Gou Y, Jaeger D, St-Pierre F
  TITLE     Widefield imaging of rapid pan-cortical voltage dynamics with an 
            indicator evolved for one-photon microscopy.
  JOURNAL   Nat Commun. 2023 Oct 12;14(1):6423. doi: 10.1038/s41467-023-41975-3.
  PUBMED    37828037
REFERENCE   2  (bases 1 to 6034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-10-12"; volume: "14(1):6423. doi"; pages: " 
            10.1038/s41467-023-41975-3"
COMMENT     Sequence Label: pAAV-GFAP-JEDI-1P-WPRE
FEATURES             Location/Qualifiers
     source          1..6034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     misc_feature    2161..2749
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2214..2234)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2632..2643)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2781..3257
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2980..2999)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3297..3437
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3297..3426
                     /label=AAV2 ITR
     rep_origin      3512..3967
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3599..3618)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3809..3830
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3984..4003)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4103..4125
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4163..4181)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4249..4353
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4354..4422,4423..5214)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5385..5973
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5874..5893
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgtct agagatctaa catatcctgg tgtggagtag
      181 gggacgctgc tctgacagag gctcgggggc ctgagctggc tctgtgagct ggggaggagg
      241 cagacagcca ggccttgtct gcaagcagac ctggcagcat tgggctggcc gccccccagg
      301 gcctcctctt catgcccagt gaatgactca ccttggcaca gacacaatgt tcggggtggg
      361 cacagtgcct gcttcccgcc gcaccccagc ccccctcaaa tgccttccga gaagcccatt
      421 gagcaggggg cttgcattgc accccagcct gacagcctgg catcttggga taaaagcagc
      481 acagccccct aggggctgcc cttgctgtgt ggcgccaccg gcggtggaga acaaggctct
      541 attcagcctg tgcccaggaa aggggatcag gggatgccca ggcatggaca gtgggtggca
      601 gggggggaga ggagggctgt ctgcttccca gaagtccaag gacacaaatg ggtgagggga
      661 gagctctccc catagctggg ctgcggccca accccacccc ctcaggctat gccagggggt
      721 gttgccaggg gcacccgggc atcgccagtc tagcccactc cttcataaag ccctcgcatc
      781 ccaggagcga gcagagccag agcaggttgg agaggagacg catcacctcc gctgctcgcg
      841 gggtctagag gatccatgga gacgactgtg aggtatgaac aggggtcaga gctcactaaa
      901 acttcgagct ctccaacagc agatgagccc acgataaaga ttgatgatgg tcgtgatgag
      961 ggtaatgaac aagacagctg ttccaatacc attaggagaa aaatttcccc gtttgtgatg
     1021 tcatttggat tcagagtatt tggagttgtg cttatcattg tagacatcat agtggtgatt
     1081 gtggatctgg ccatcagtga gaagaaaaga ggcattagag agattcttga aggtgtttcc
     1141 ctggctatag cactcttctt ccttgttgat gttctcatga gagtgtttgt tgaaggcttc
     1201 aagaactatt tccggtccaa actggttact ttggatgcag tcatagtagt gggcactctg
     1261 ctaattaata tgacctactc cttctctgac cttgctgcct ttaacagcga gaacgtgtat
     1321 attaccgcgg ataaacagaa aaacggcatt aaagcgaact ttaccgtgcg ccataacgtg
     1381 gaagatggca gcgtgcagct ggcggatcat tatcagcaga acaccccgat tggcgatggc
     1441 ccggtgctgc tgccggataa ccattatctg agcacccaga ccgtgctgag caaagatccg
     1501 aacgaaaaac gcgatcacat ggtgctgctg gaatttgtga ccgcagcggg cattacacac
     1561 ggcatggatg aactgtatgg cggcaccggc ggcagcgcga gccagggcga agaactgttt
     1621 accggcgtgg tgccgattct ggtggaactg gatggcgatg tgaacggcca taaatttagc
     1681 gtgcgcggcg aaggcgaagg cgatgcgacc attggcaaac tgaccctgaa atttatttgc
     1741 accaccggca aactaccggt gccgtggccg accctggtga ccaccttaac ctatggcgtg
     1801 cagtgcttta gccgctatcc ggatcatatg aaacgccatg atttttttaa aagcgcgatg
     1861 ccggaaggct atgtgcagga acgcaccatt agctttaaag atgatggcaa atataaaacc
     1921 cgcgcggtgg tgaaatttga aggcgatacc ctggtgaacc gcattgaact gaaaggcacc
     1981 gattttaaag aagatggcaa cattctgggg cataaactgg aatataacac agatcagatg
     2041 ccgcacatgg ttactcttct tcgagttctg aaaattgtta tcttaataag aatatttcgc
     2101 ctggcttcac agaagaaaca acttgaagtg gtaacctaat gagatatcaa gcttatcgat
     2161 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     2221 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     2281 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     2341 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     2401 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     2461 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     2521 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     2581 gcctatgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     2641 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     2701 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga
     2761 gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg
     2821 ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca
     2881 ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca
     2941 aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga
     3001 gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc
     3061 tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt
     3121 tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat
     3181 ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg
     3241 ctcccttccc tgtccttctg attttgtagg taaccacgtg cggaccgagc ggccgcagga
     3301 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     3361 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     3421 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     3481 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     3541 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
     3601 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3661 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3721 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     3781 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     3841 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
     3901 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     3961 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     4021 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4081 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4141 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4201 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4261 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4321 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4381 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4441 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4501 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4561 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4621 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4681 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4741 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4801 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4861 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4921 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4981 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5041 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5101 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5161 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5221 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5281 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5341 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5401 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5461 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5521 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5581 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5641 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5701 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5761 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5821 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5881 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5941 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6001 ttcctggcct tttgctggcc ttttgctcac atgt
//
