LOCUS       sequence-397650-       12691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12691)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0919
FEATURES             Location/Qualifiers
     source          1..12691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8548..9136
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8601..8621)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9019..9030)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9208..9441
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9519..9640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9556..9575)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9610..9629
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9680..9815
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9835..9854)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9836..9854)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9864..9881)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9864..9880)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9873..9895)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10022..10477
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10109..10128)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10319..10340
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10503..10607
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10608..10676,10677..11468)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10826..10845)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11639..12227
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12128..12147
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12381..12398
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12515..12536
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12551..12556,12557..12574,12575..12581)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12589..12605
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12594..12616
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12613..12629
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12613..12629
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12648..12668
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12650..12668
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgagt ggcctcgggg acagttcatc
     4141 cgaccctgct aacccagact cacataagag gaaaggatcg ccatgtgaca cactggcatc
     4201 aagcacggaa aagaggcgca gggagcaaga aaataaatat ttagaagaac tagctgagtt
     4261 actgtctgcc aacattagtg acattgacag cttgagtgta aaaccagaca aatgcaagat
     4321 tttgaagaaa acagtcgatc agatacagct aatgaagaga atggaacaag agaaatcaac
     4381 aactgatgac gatgtacaga aatcagacat ctcatcaagt agtcaaggag tgatagaaaa
     4441 ggaatccttg ggacctcttc ttttggaggc tttggatgga tttttctttg ttgtgaactg
     4501 tgaagggaga attgtatttg tgtcagagaa tgtaaccagc tacttaggtt acaatcagga
     4561 ggaattaatg aatacgagcg tctacagcat actgcacgtg ggggatcatg cagaatttgt
     4621 gaagaatctg ctaccaaaat cactagtaaa tggagttcct tggcctcaag aggcaacacg
     4681 acgaaatagc cataccttta actgcaggat gctaattcac cctccagatg agccagggac
     4741 cgagaaccaa gaagcttgcc agcgttatga agtaatgcag tgtttcactg tgtcacagcc
     4801 aaaatcaatt caagaggatg gagaagattt ccagtcatgt ctgatttgta ttgcacggcg
     4861 attacctcgg cctccagcta ttacgggtgt agaatccttt atgaccaagc aagatactac
     4921 aggtaaaatc atctctattg atactagttc cctgagagct gctggcagaa ctggttggga
     4981 agatttagtg aggaagtgca tttatgcttt tttccaacct cagggcagag aaccatctta
     5041 tgccagacag ctgttccaag aagtgatgac tcgtggcact gcctccagcc cctcctatag
     5101 attcatattg aatgatggga caatgcttag cgcccacacc aagtgtaaac tttgctaccc
     5161 tcaaagtcca gacatgcaac ctttcatcat gggaattcat atcatcgaca gggagcacag
     5221 tgggctttct cctcaagatg acactaattc tggaatgtca attccccgag taaatccctc
     5281 ggtcaatcct agtatctctc cagctcatgg tgtggctcgt tcatccacat tgccaccatc
     5341 caacagcaac atggtatcca ccagaataaa ccgccagcag agctcagacc ttcatagcag
     5401 cagtcatagt aattctagca acagccaagg aagtttcgga tgctcacccg gaagtcagat
     5461 tgtagccaat gttgccttaa accaaggaca ggccagttca cagagcagta atccctcttt
     5521 aaacctcaat aattctccta tggaaggtac aggaatatcc ctagcacagt tcatgtctcc
     5581 aaggagacag gttacttctg gattggcaac aaggcccagg atgccaaaca attcctttcc
     5641 tcctaatatt tcgacattaa gctctcccgt tggcatgaca agtagtgcct gtaataataa
     5701 taaccgatct tattcaaaca tcccagtaac atctttacag ggtatgaatg aaggacccaa
     5761 taactccgtt ggcttctctg ccagttctcc agtcctcagg cagatgagct cacagaattc
     5821 acctagcaga ttaaatatac aaccagcaaa agctgagtcc aaagataaca aagagattgc
     5881 ctcaatttta aatgaaatga ttcaatctga caacagctct agtgatggca aacctctgga
     5941 ttcagggctt ctgcataaca atgacagact ttcagatgga gacagtaaat actctcaaac
     6001 cagtcacaaa ctagtgcagc ttttgacaac aactgccgaa cagcagttac ggcatgctga
     6061 tatagacaca agctgcaaag atgtcctgtc ttgcacaggc acttccaact ctgcctctgc
     6121 taactcttca ggaggttctt gtccctcttc tcatagctca ttgacagaac ggcataaaat
     6181 tctacaccgg ctcttacagg agggtagccc ctcagatatc accactttgt ctgtcgagcc
     6241 tgataaaaag gacagtgcat ctacttctgt gtcagtgact ggacaggtac aaggaaactc
     6301 cagtataaaa ctagaactgg atgcttcaaa gaaaaaagaa tcaaaagacc atcagctcct
     6361 acgctatctt ttagataaag atgagaaaga tttaagatca actccaaacc tgagcctgga
     6421 tgatgtaaag gtgaaagtgg aaaagaaaga acagatggat ccatgtaata caaacccaac
     6481 cccaatgacc aaacccactc ctgaggaaat aaaactggag gcccagagcc agtttacagc
     6541 tgaccttgac cagtttgatc agttactgcc cacgctggag aaggcagcac agttgccagg
     6601 cttatgtgag acagacagga tggatggtgc ggtcaccagt gtaaccatca aatcggagat
     6661 cctgccagct tcacttcagt ccgccactgc cagacccact tccaggctaa atagattacc
     6721 tgagctggaa ttggaagcaa ttgataacca atttggacaa ccaggaacag gcgatcagat
     6781 tccatggaca aataatacag tgacagctat aaatcagagt aaatcagaag accagtgtat
     6841 tagctcacaa ttagatgagc ttctctgtcc acccacaaca gtagaaggga gaaatgatga
     6901 gaaggctctt cttgaacagc tggtatcctt ccttagtggc aaagatgaaa ctgagctagc
     6961 tgaactagac agagctctgg gaattgacaa acttgttcag gggggtggat tagatgtatt
     7021 atcagagaga tttccaccac aacaagcaac gccacctttg atcatggaag aaagacccaa
     7081 cctttattcc cagccttact cttctccttc tcctactgcc aatctcccta gccctttcca
     7141 aggcatggtc aggcaaaaac cttcactggg gacgatgcct gttcaagtaa cacctccccg
     7201 aggtgctttt tcacctggca tgggcatgca gcccaggcaa actctaaaca gacctccggc
     7261 tgcacctaac cagcttcgac ttcaactaca gcagcgatta cagggacaac agcagttgat
     7321 acaccaaaat cggcaagcta tcttaaacca gtttgcagca actgctcctg ttggcatcaa
     7381 tatgagatca ggcatgcaac agcaaattac acctcagcca cccctgaatg ctcaaatgtt
     7441 ggcacaacgt cagcgggaac tgtacagtca acagcaccga cagaggcagc taatacagca
     7501 gcaaagagcc atgcttatga ggcagcaaag ctttgggaac aacctccctc cctcatctgg
     7561 actaccagtt caaatgggga acccccgtct tcctcagggt gctccacagc aattccccta
     7621 tccaccaaac tatggtacaa atccaggaac cccacctgct tctaccagcc cgttttcaca
     7681 actagcagca aatcctgaag catccttggc caaccgcaac agcatggtga gcagaggcat
     7741 gacaggaaac ataggaggac agtttggcac tggaatcaat cctcagatgc agcagaatgt
     7801 cttccagtat ccaggagcag gaatggttcc ccaaggtgag gccaactttg ctccatctct
     7861 aagccctggg agctccatgg tgccgatgcc aatccctcct cctcagagtt ctcttctcca
     7921 gcaaactcca cctgcctccg ggtatcagtc accagacatg aaggcctggc agcaaggagc
     7981 gataggaaac aacaatgtgt tcagtcaagc tgtccagaac cagcccacgc ctgcacagcc
     8041 aggagtatac aacaacatga gcatcaccgt ttccatggca ggtggaaata cgaatgttca
     8101 gaacatgaac ccaatgatgg cccagatgca gatgagctct ttgcagatgc caggaatgaa
     8161 cactgtgtgc cctgagcaga taaatgatcc cgcactgaga cacacaggcc tctactgcaa
     8221 ccagctctca tccactgacc ttctcaaaac agaagcagat ggaacccagc aggtgcaaca
     8281 ggttcaggtg tttgctgacg tccagtgtac agtgaatctg gtaggcgggg acccttacct
     8341 gaaccagcct ggtccactgg gaactcaaaa gcccacgtca ggaccacaga ccccccaggc
     8401 ccagcagaag agcctccttc agcagctact gactgaatag taatgaacta gtccgtaact
     8461 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgatc tgggcgatac
     8521 ttctcccata aaacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     8581 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     8641 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     8701 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     8761 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     8821 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     8881 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     8941 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     9001 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     9061 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     9121 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     9181 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     9241 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     9301 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9361 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     9421 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     9481 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     9541 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9601 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     9661 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     9721 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     9781 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     9841 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     9901 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     9961 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
    10021 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
    10081 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
    10141 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
    10201 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
    10261 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
    10321 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
    10381 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
    10441 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
    10501 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
    10561 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
    10621 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    10681 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    10741 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    10801 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    10861 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    10921 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    10981 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    11041 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    11101 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    11161 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    11221 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    11281 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    11341 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    11401 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    11461 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    11521 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    11581 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    11641 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    11701 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    11761 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    11821 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    11881 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    11941 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    12001 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    12061 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    12121 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    12181 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    12241 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    12301 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    12361 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    12421 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    12481 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    12541 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    12601 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    12661 actaaaggga acaaaagctg gagctgcaag c
//
