LOCUS       sequence-397657-       10945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10945)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0939
FEATURES             Location/Qualifiers
     source          1..10945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6802..7390
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6855..6875)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7273..7284)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7462..7695
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7773..7894
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7810..7829)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7864..7883
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7934..8069
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8089..8108)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8090..8108)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8118..8135)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8118..8134)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8127..8149)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8276..8731
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8363..8382)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8573..8594
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8757..8861
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8862..8930,8931..9722)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9080..9099)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9893..10481
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10382..10401
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10635..10652
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10769..10790
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10805..10810,10811..10828,10829..10835)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10843..10859
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10848..10870
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10867..10883
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10867..10883
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10902..10922
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10904..10922
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggac ttcaccttgg gagactggga
     4141 gcagctcggg ctggaacagg gggacacgtt ctgggacaca gcgttggaca attgccagga
     4201 cctcttcctg ctggaccccc caagacccaa cctgacctcc cacccagatg gcagtgaaga
     4261 tctggagcct ctggcaggag gaagcccaga agcaacaagc cctgatgtga ctgagaccaa
     4321 gaactctcct ctgatggagg atttcttcga agaaggattc tcccaggaga ttatagagat
     4381 gttatccaag gatggcttct ggaactccaa tttcggagaa gcctgtatag aggacacctg
     4441 gttagatagt ttgctaggcg atccagaaag tcttctgagg tctgatattg ccaccaacgg
     4501 ggaaagtccc acggaatgca agagtcatga attaaagaga ggactcagtc ctgtgtccac
     4561 cgtttccacg ggagaagatt ccatggtgca taatgtttct gaaaagaccc tcacaccagc
     4621 taagtctaag gaatataggg gtgagttttt ctcctactcc gaccacagcc agcaggattc
     4681 tgttcaggaa ggggagaaac catatcaatg tagtgaatgt gggaaaagct tcagtgggag
     4741 ttaccgtctt acccagcact ggatcactca tactagggag aaacccactg tccatcaaga
     4801 gtgtgagcaa ggttttgacc ggaatgcttc cctttctgtg tatccgaaaa ctcacacggg
     4861 ctacaaattc tatgtgtgta atgaatatgg gacaactttt agtcagagta catacctgtg
     4921 gcatcagaaa actcacactg gagaaaaacc atgtaagagt caagatagtg accacccacc
     4981 cagtcatgac acacagcctg gtgagcatca gaaaactcac acagatagta agtcctacaa
     5041 ctgtaacgaa tgcggcaagg cttttacccg gatcttccac cttactcggc accagaagat
     5101 ccacactcgg aaacgctatg agtgttccaa gtgccaggcg accttcaact tgagaaaaca
     5161 cctcatccaa catcagaaaa ctcacgctgc aaaaactacc tctgagtgtc aggagtgtgg
     5221 gaagattttt aggcacagtt cgctgctcat tgaacaccag gctcttcatg ctggagagga
     5281 gccttataag tgtaacgaac gtgggaaatc cttcaggcat aactctaccc taaagatcca
     5341 tcagagggtt cacagtggag agaagcctta caaatgcagt gagtgtggga aggccttcca
     5401 ccggcacact caccttaatg aacatcggcg aattcataca ggctacagac cccacaaatg
     5461 tcaggaatgc gtcaggagtt tcagccggcc ctcacatctg atgcgacatc aggccattca
     5521 caccgcagaa aagccctata gctgtgctga atgcaaggag actttcagcg ataacaatcg
     5581 ccttgtgcaa caccagaaaa tgcacactgt caaaacccca tatgaatgtc aggagtgcgg
     5641 agaacgcttc atttgcggct caaccctgaa gtgccacgag agtgttcacg ccagagaaaa
     5701 acaaggattt tttgtgagtg ggaagatctt ggatcagaac ccagaacaga aagagaagtg
     5761 ctttaagtgt aacaaatgtg agaaaacctt tagctgcagc aaatacctaa ctcagcacga
     5821 gaggattcac accaggggag tgaagccctt tgaatgtgac cagtgtggga aagcctttgg
     5881 ccaaagtact cggctcattc accatcaaag aatccactct agagtgaggc tgtataaatg
     5941 gggtgagcaa gggaaagcca tcagcagtgc ctcccttatc aaacttcagt ccttccacac
     6001 aaaggagcac ccttttaaat gtaacgaatg cggaaagacc ttcagccaca gtgcacacct
     6061 ctcaaaacat cagttaattc acgctggaga gaatcccttt aaatgtagta agtgtgacag
     6121 agtcttcacc cagagaaact accttgttca gcatgagcga actcatgcca gaaagaagcc
     6181 gttggtgtgt aacgaatgcg ggaaaacgtt ccgtcagagc tcatgccttt ctaagcatca
     6241 gagaattcac tcaggtgaga agccctatgt atgtgattac tgcgggaagg ccttcggcct
     6301 gagtgctgag cttgtccgcc accagagaat tcacactgga gaaaagcctt atgtttgtca
     6361 ggaatgcggg aaagccttca cccagagctc atgcctttct attcaccgga gagttcacac
     6421 tggggagaag ccctacagat gtggtgaatg tgggaaagcc tttgcccaga aagcaaatct
     6481 aacacagcac cagagaattc acacagggga gaagccttac tcctgtaatg tgtgtggcaa
     6541 agcttttgtc ctcagtgccc atctcaacca gcacctgaga gttcacaccc aggagacact
     6601 ttatcagtgt caacgttgcc agaaagcctt tcggtgccac tcgagcctca gccgccatca
     6661 gcgtgtacac aacaagcagc aatactgcct gtagtaatga actagtccgt aacttgaaag
     6721 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaatcgagag gtcagacaca
     6781 tttaccacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     6841 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6901 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6961 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7021 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7081 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7141 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7201 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7261 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7321 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7381 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     7441 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     7501 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7561 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7621 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7681 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     7741 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     7801 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7861 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     7921 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7981 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     8041 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     8101 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     8161 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     8221 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     8281 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     8341 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     8401 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     8461 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     8521 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     8581 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     8641 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     8701 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     8761 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8821 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8881 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     8941 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     9001 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     9061 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     9121 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     9181 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9241 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9301 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9361 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9421 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9481 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9541 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     9601 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9661 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9721 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9781 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9841 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     9901 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     9961 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    10021 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    10081 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    10141 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    10201 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10261 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    10321 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10381 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10441 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10501 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    10561 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    10621 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    10681 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    10741 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    10801 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    10861 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    10921 gggaacaaaa gctggagctg caagc
//
