LOCUS       sequence-397675-       13078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13078)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2768
FEATURES             Location/Qualifiers
     source          1..13078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8935..9523
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8988..9008)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9406..9417)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9595..9828
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9906..10027
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9943..9962)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9997..10016
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10067..10202
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10222..10241)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10223..10241)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10251..10268)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10251..10267)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10260..10282)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10409..10864
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10496..10515)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10706..10727
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10890..10994
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10995..11063,11064..11855)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11213..11232)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12026..12614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12515..12534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12768..12785
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12902..12923
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12938..12943,12944..12961,12962..12968)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12976..12992
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12981..13003
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13000..13016
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13000..13016
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13035..13055
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13037..13055
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggaa ccggggtgtg acgagttcct
     4141 gccgccaccg gagtgcccgg tttttgagcc tagctgggct gaattccaag acccgcttgg
     4201 ctacattgcg aaaataaggc ccatagcaga gaagtctggc atctgcaaaa tccgcccacc
     4261 cgcggattgg cagcctcctt ttgcagtaga agttgacaat ttcagattta ctcctcgcgt
     4321 ccaaaggcta aatgaactgg aggcccaaac tagagtgaaa ttgaactatt tggatcagat
     4381 tgcaaaattc tgggaaattc aaggctcctc tttaaagatt cccaatgtgg agcggaagat
     4441 cttggacctc tacagcctta gtaagattgt gattgaggaa ggtggctatg aagccatctg
     4501 caaggatcgt cggtgggctc gagttgccca gcgtctccac tacccaccag gcaaaaacat
     4561 tggctccctg ctacgatcac attacgaacg cattatttac ccctatgaaa tgtttcagtc
     4621 tggagccaac catgtgcaat gtaacacaca cccgtttgac aatgaggtaa aagataagga
     4681 atacaagccc cacagcatcc cccttagaca gtctgtgcag ccttcaaagt tcagcagcta
     4741 cagtcgacgg gcaaaaaggc tacagcctga tccagagcct acagaggagg acattgagaa
     4801 gcatccagag ctaaagaagt tacagatata tgggccaggt cccaaaatga tgggcttggg
     4861 ccttatggct aaggataagg ataagactgt gcataagaaa gtcacatgcc ccccaactgt
     4921 tacggtgaag gatgagcaaa gtggaggtgg gaacgtgtca tcaacattgc tcaagcagca
     4981 cttgagccta gagccctgca ctaagacaac catgcaactt cgaaagaatc acagcagtgc
     5041 ccagtttatt gactcatata tttgccaagt atgctcccgt ggggatgaag atgataagct
     5101 tcttttctgt gatggctgtg atgacaatta ccacatcttc tgcttgttac caccccttcc
     5161 tgaaatcccc agaggcatct ggaggtgccc aaaatgtatc ttggcggagt gtaaacagcc
     5221 tcctgaagct tttggatttg aacaggctac ccaggagtac agtttgcaga gttttggtga
     5281 aatggctgat tccttcaagt ccgactactt caacatgcct gtacatatgg tgcctacaga
     5341 acttgtagag aaggaattct ggaggctggt gagcagcatt gaggaagacg tgacagttga
     5401 atatggagct gatattcatt ccaaagaatt tggcagtggc tttcctgtca gcaatagcaa
     5461 acaaaactta tctcctgagg agaagagaca aagtctcact gtgttgacca ggctgatctc
     5521 aagcttctgg gctcaagcag tcctcccacc ttggcctccc aaagtgttgg gattacagga
     5581 gtatgcgacc agtggttgga acctgaatgt gatgccagtg ctagatcagt ctgttctctg
     5641 tcacatcaat gcagacatct caggcatgaa ggtgccctgg ctgtacgtgg gcatggtttt
     5701 ctcagcattt tgttggcata ttgaggatca ctggagttac tctattaact atctgcattg
     5761 gggtgagccg aagacctggt atggtgtacc ctccctggca gcagagcatt tggaggaggt
     5821 gatgaagatg ctgacacctg agctgtttga tagccagcct gatctcctac accagcttgt
     5881 cactctcatg aatcccaaca ctttgatgtc ccatggtgtg ccagttgtcc gcacaaacca
     5941 gtgtgcaggg gagtttgtca tcacttttcc tcgtgcttac cacagtggtt ttaaccaagg
     6001 ctacaatttt gctgaagctg tcaacttttg tactgctgac tggctacctg ctggacgcca
     6061 gtgcattgaa cactaccgcc ggctccggcg ctattgtgtc ttctcccacg aggagctcat
     6121 ctgcaagatg gctgccttcc cagagacgtt ggatctcaat ctagcagtag ctgtgcacaa
     6181 ggagatgttc attatggttc aggaggagcg acgtctacga aaggcccttt tggagaaggg
     6241 cgtcacggag gctgagcgag aggcttttga gctgctccca gatgatgaac gccagtgcat
     6301 caagtgcaag accacgtgct tcttgtcagc cctggcctgc tacgactgcc cagatggcct
     6361 tgtatgcctt tcccacatca atgacctctg caagtgctct agtagccgac agtacctccg
     6421 gtatcggtac accttggatg agctccccac catgctgcat aaactgaaga ttcgggctga
     6481 gtcttttgac acctgggcca acaaagtgcg agtggccttg gaggtggagg atggccgtaa
     6541 acgcagcttt gaagagctaa gggcactgga gtctgaggct cgtgagagga ggtttcctaa
     6601 tagtgagctg cttcagcgac tgaagaactg cctgagtgag gtggaggctt gtattgctca
     6661 agtcctgggg ctggtcagtg gtcaggtggc caggatggac actccacagc tgaccttgac
     6721 tgaactccgg gtccttcttg agcagatggg cagcctgccc tgtgccatgc atcagattgg
     6781 ggatgtcaag gatgtcctgg aacaggtgga ggcctatcaa gctgaggctc gtgaggctct
     6841 ggccacactg ccctctagtc cagggctatt gcggtccctg ttggagaggg ggcagcagct
     6901 gggtgtagag gtgcctgaag cccatcagct tcagcagcag gtggagcagg cgcaatggct
     6961 agatgaagtg aagcaggccc tggccccttc tgctcacagg ggctctctgg tcatcatgca
     7021 ggggcttttg gttatgggtg ccaagatagc ctccagccct tctgtggaca aggcccgggc
     7081 tgagctgcaa gaactactga ccattgcaga gcgctgggaa gaaaaggctc atttctgcct
     7141 ggaggccagg cagaagcatc caccagccac attggaagcc ataattcgtg agacagaaaa
     7201 catccctgtt cacctgccta acatccaggc tctcaaagaa gctctgacta aggcacaagc
     7261 ttggattgct gatgtggatg agatccaaaa tggtgaccac tacccctgtc tagatgactt
     7321 ggagggcctg gtggctgtgg gccgggacct gcctgtgggg ctggaagagc tgagacagct
     7381 agagctgcag gtattgacag cacattcctg gagagagaag gcctccaaga cctttctcaa
     7441 gaagaattct tgctacacac tgcttgaggt gctttgcccg tgtgcagacg ctggctcaga
     7501 cagcaccaag cgtagccggt ggatggagaa ggcgctgggg ttgtaccagt gtgacacaga
     7561 gctgctgggg ctgtctgcac aggacctcag agacccaggc tctgtgattg tggccttcaa
     7621 ggaaggggaa cagaaggaga aggagggtat cctgcagctg cgtcgcacca actcagccaa
     7681 gcccagtcca ctggcaccat ccctcatggc ctcttctccg acttctatct gtgtgtgtgg
     7741 gcaggtgcca gctggggtgg gagttctgca gtgtgacctg tgtcaggact ggttccatgg
     7801 gcagtgtgtg tcagtgcccc atctcctcac ctctccaaag cccagtctca cttcatctcc
     7861 actgctagcc tggtgggaat gggacacaaa attcctgtgt ccactgtgta tgcgctcacg
     7921 acggccacgc ctagagacaa tcctagcctt gctggttgcc ctgcagaggc tgcccgtgcg
     7981 gctgcctgag ggtgaggccc ttcagtgtct cacagagagg gccattggct ggcaagaccg
     8041 tgccagaaag gctctggcct ctgaagatgt gactgctctg ttgcgacagc tggctgagct
     8101 tcgccaacag ctacaggcca aacccagacc agaggaggcc tcagtctaca cttcagccac
     8161 tgcctgtgac cctatcagag aaggcagtgg caacaatatt tctaaggtcc aagggctgct
     8221 ggagaatgga gacagtgtga ccagtcctga gaacatggct ccaggaaagg gctctgacct
     8281 ggagctactg tcctcactgt tgccgcagtt gactggccct gtgttggagc tgcctgaggc
     8341 aatccgggct cccctggagg agctcatgat ggaaggggac ctgcttgagg tgaccctgga
     8401 tgagaaccac agcatctggc agctgctgca ggctggacag cctccagacc tggacagaat
     8461 tcgcacactt ctggagctgg aaaaatttga acatcaaggg agtcggacaa ggagccgggc
     8521 tctggagagg cgacggcggc ggcagaaggt ggatcagggt agaaacgttg agaatcttgt
     8581 tcaacaggag cttcagtcaa aaagggctcg gagctcaggg attatgtctc aggtgggccg
     8641 agaagaagaa cattatcagg agaaagcaga ccgtgaaaat atgttcctga caccttccac
     8701 agaccacagc cctttcttga aaggaaacca aaatagctta caacacaagg attcaggctc
     8761 ttcagctgct tgtccttctt taatgccttt gctacaactc tcctactctg atgagcaaca
     8821 gttgtagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     8881 ttgtggaaag gacgaatatg gagtgaagtt tagctcaata cgcgttaagt cgacaatcaa
     8941 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     9001 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     9061 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     9121 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     9181 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     9241 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     9301 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     9361 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     9421 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     9481 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     9541 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     9601 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     9661 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9721 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9781 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     9841 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     9901 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     9961 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    10021 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
    10081 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
    10141 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    10201 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
    10261 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
    10321 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
    10381 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
    10441 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
    10501 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    10561 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    10621 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    10681 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    10741 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    10801 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
    10861 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    10921 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    10981 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    11041 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    11101 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    11161 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    11221 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
    11281 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    11341 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    11401 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    11461 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    11521 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    11581 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    11641 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    11701 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    11761 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    11821 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    11881 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    11941 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    12001 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    12061 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    12121 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    12181 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    12241 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    12301 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    12361 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    12421 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    12481 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    12541 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    12601 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    12661 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    12721 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    12781 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    12841 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    12901 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    12961 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    13021 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagc
//
