LOCUS       sequence-397690-       12565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12565)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0918
FEATURES             Location/Qualifiers
     source          1..12565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8422..9010
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8475..8495)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8893..8904)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9082..9315
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9393..9514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9430..9449)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9484..9503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9554..9689
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9709..9728)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9710..9728)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9738..9755)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9738..9754)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9747..9769)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9896..10351
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9983..10002)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10193..10214
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10377..10481
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10482..10550,10551..11342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10700..10719)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11513..12101
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12002..12021
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12255..12272
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12389..12410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12425..12430,12431..12448,12449..12455)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12463..12479
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12468..12490
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12487..12503
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12487..12503
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12522..12542
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12524..12542
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgagt ggcctcgggg acagttcatc
     4141 cgaccctgct aacccagact cacataagag gaaaggatcg ccatgtgaca cactggcatc
     4201 aagcacggaa aagaggcgca gggagcaaga aaataaatat ttagaagaac tagctgagtt
     4261 actgtctgcc aacattagtg acattgacag cttgagtgta aaaccagaca aatgcaagat
     4321 tttgaagaaa acagtcgatc agatacagct aatgaagaga atggaacaag agaaatcaac
     4381 aactgatgac gatgtacaga aatcagacat ctcatcaagt agtcaaggag tgatagaaaa
     4441 ggaatccttg ggacctcttc ttttggaggc tttggatgga tttttctttg ttgtgaactg
     4501 tgaagggaga attgtatttg tgtcagagaa tgtaaccagc tacttaggtt acaatcagga
     4561 ggaattaatg aatacgagcg tctacagcat actgcacgtg ggggatcatg cagaatttgt
     4621 gaagaatctg ctaccaaaat cactagtaaa tggagttcct tggcctcaag aggcaacacg
     4681 acgaaatagc cataccttta actgcaggat gctaattcac cctccagatg agccagggac
     4741 cgagaaccaa gaagcttgcc agcgttatga agtaatgcag tgtttcactg tgtcacagcc
     4801 aaaatcaatt caagaggatg gagaagattt ccagtcatgt ctgatttgta ttgcacggcg
     4861 attacctcgg cctccagcta ttacgggtgt agaatccttt atgaccaagc aagatactac
     4921 aggtaaaatc atctctattg atactagttc cctgagagct gctggcagaa ctggttggga
     4981 agatttagtg aggaagtgca tttatgcttt tttccaacct cagggcagag aaccatctta
     5041 tgccagacag ctgttccaag aagtgatgac tcgtggcact gcctccagcc cctcctatag
     5101 attcatattg aatgatggga caatgcttag cgcccacacc aagtgtaaac tttgctaccc
     5161 tcaaagtcca gacatgcaac ctttcatcat gggaattcat atcatcgaca gggagcacag
     5221 tgggctttct cctcaagatg acactaattc tggaatgtca attccccgag taaatccctc
     5281 ggtcaatcct agtatctctc cagctcatgg tgtggctcgt tcatccacat tgccaccatc
     5341 caacagcaac atggtatcca ccagaataaa ccgccagcag agctcagacc ttcatagcag
     5401 cagtcatagt aattctagca acagccaagg aagtttcgga tgctcacccg gaagtcagat
     5461 tgtagccaat gttgccttaa accaaggaca ggccagttca cagagcagta atccctcttt
     5521 aaacctcaat aattctccta tggaaggtac aggaatatcc ctagcacagt tcatgtctcc
     5581 aaggagacag gttacttctg gattggcaac aaggcccagg atgccaaaca attcctttcc
     5641 tcctaatatt tcgacattaa gctctcccgt tggcatgaca agtagtgcct gtaataataa
     5701 taaccgatct tattcaaaca tcccagtaac atctttacag ggtatgaatg aaggacccaa
     5761 taactccgtt ggcttctctg ccagttctcc agtcctcagg cagatgagct cacagaattc
     5821 acctagcaga ttaaatatac aaccagcaaa agctgagtcc aaagataaca aagagattgc
     5881 ctcaatttta aatgaaatga ttcaatctga caacagctct agtgatggca aacctctgga
     5941 ttcagggctt ctgcataaca atgacagact ttcagatgga gacagtaaat actctcaaac
     6001 cagtcacaaa ctagtgcagc ttttgacaac aactgccgaa cagcagttac ggcatgctga
     6061 tatagacaca agctgcaaag atgtcctgtc ttgcacaggc acttccaact ctgcctctgc
     6121 taactcttca ggaggttctt gtccctcttc tcatagctca ttgacagaac ggcataaaat
     6181 tctacaccgg ctcttacagg agggtagccc ctcagatatc accactttgt ctgtcgagcc
     6241 tgataaaaag gacagtgcat ctacttctgt gtcagtgact ggacaggtac aaggaaactc
     6301 cagtataaaa ctagaactgg atgcttcaaa gaaaaaagaa tcaaaagacc atcagctcct
     6361 acgctatctt ttagataaag atgagaaaga tttaagatca actccaaacc tgagcctgga
     6421 tgatgtaaag gtgaaagtgg aaaagaaaga acagatggat ccatgtaata caaacccaac
     6481 cccaatgacc aaacccactc ctgaggaaat aaaactggag gcccagagcc agtttacagc
     6541 tgaccttgac cagtttgatc agttactgcc cacgctggag aaggcagcac agttgccagg
     6601 cttatgtgag acagacagga tggatggtgc ggtcaccagt gtaaccatca aatcggagat
     6661 cctgccagct tcacttcagt ccgccactgc cagacccact tccaggctaa atagattacc
     6721 tgagctggaa ttggaagcaa ttgataacca atttggacaa ccaggaacag gcgatcagat
     6781 tccatggaca aataatacag tgacagctat aaatcagagt aaatcagaag accagtgtat
     6841 tagctcacaa ttagatgagc ttctctgtcc acccacaaca gtagaaggga gaaatgatga
     6901 gaaggctctt cttgaacagc tggtatcctt ccttagtggc aaagatgaaa ctgagctagc
     6961 tgaactagac agagctctgg gaattgacaa acttgttcag gggggtggat tagatgtatt
     7021 atcagagaga tttccaccac aacaagcaac gccacctttg atcatggaag aaagacccaa
     7081 cctttattcc cagccttact cttctccttc tcctactgcc aatctcccta gccctttcca
     7141 aggcatggtc aggcaaaaac cttcactggg gacgatgcct gttcaagtaa cacctccccg
     7201 aggtgctttt tcacctggca tgggcatgca gcccaggcaa actctaaaca gacctccggc
     7261 tgcacctaac cagcttcgac ttcaactaca gcagcgatta cagggacaac agcagttgat
     7321 acaccaaaat cggcaagcta tcttaaacca gtttgcagca actgctcctg ttggcatcaa
     7381 tatgagatca ggcatgcaac agcaaattac acctcagcca cccctgaatg ctcaaatgtt
     7441 ggcacaacgt cagcgggaac tgtacagtca acagcaccga cagaggcagc taatacagca
     7501 gcaaagagcc atgcttatga ggcagcaaag ctttgggaac aacctccctc cctcatctgg
     7561 actaccagtt caaatgggga acccccgtct tcctcagggt gctccacagc aattccccta
     7621 tccaccaaac tatggtacaa atccaggaac cccacctgct tctaccagcc cgttttcaca
     7681 actagcagca aatcctgaag catccttggc caaccgcaac agcatggtga gcagaggcat
     7741 gacaggaaac ataggaggac agtttggcac tggaatcaat cctcagatgc agcagaatgt
     7801 cttccagtat ccaggagcag gaatggttcc ccaaggtgag gccaactttg ctccatctct
     7861 aagccctggg agctccatgg tgccgatgcc aatccctcct cctcagagtt ctcttctcca
     7921 gcaaactcca cctgcctccg ggtatcagtc accagacatg aaggcctggc agcaaggagc
     7981 gataggaaac aacaatgtgt tcagtcaagc tgtccagaac cagcccacgc ctgcacagcc
     8041 aggagtatac aacaacatga gcatcaccgt ttccatggca ggtggaaata cgaatgttca
     8101 gaacatgaac ccaatgatgg cccagatgca gatgagctct ttgcagatgc caggaatgaa
     8161 cactgtgtgc cctgagcaga taaatgatcc cgcactgaga cacacaggcc tctactgcaa
     8221 ccagctctca tccactgacc ttctcaaaac agaagcagat ggaacccagg acaagaagac
     8281 agaagagttc ttctctgtgg tgactacaga ctagtaatga actagtccgt aacttgaaag
     8341 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaggagatag gacgcttcta
     8401 agagggacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     8461 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     8521 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     8581 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     8641 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     8701 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     8761 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     8821 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     8881 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     8941 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     9001 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     9061 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     9121 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     9181 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     9241 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     9301 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     9361 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     9421 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9481 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     9541 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     9601 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     9661 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     9721 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     9781 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     9841 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     9901 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     9961 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    10021 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    10081 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    10141 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    10201 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    10261 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    10321 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
    10381 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    10441 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    10501 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    10561 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    10621 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    10681 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    10741 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    10801 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    10861 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    10921 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    10981 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    11041 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    11101 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    11161 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    11221 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    11281 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    11341 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    11401 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    11461 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    11521 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    11581 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    11641 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    11701 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    11761 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    11821 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    11881 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    11941 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    12001 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    12061 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    12121 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    12181 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    12241 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    12301 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    12361 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    12421 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    12481 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    12541 gggaacaaaa gctggagctg caagc
//
