LOCUS       sequence-397694-       12139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12139)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0677
FEATURES             Location/Qualifiers
     source          1..12139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7996..8584
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8049..8069)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8467..8478)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8656..8889
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8967..9088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9004..9023)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9058..9077
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9128..9263
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9283..9302)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9284..9302)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9312..9329)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9312..9328)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9321..9343)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9470..9925
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9557..9576)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9767..9788
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9951..10055
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10056..10124,10125..10916)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10274..10293)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11087..11675
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11576..11595
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11829..11846
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11963..11984
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11999..12004,12005..12022,12023..12029)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12037..12053
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12042..12064
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12061..12077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12061..12077
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12096..12116
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12098..12116
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgcga tccaaggcga gggcgaggaa
     4141 gctagccaaa agtgacggtg acgttgtaaa taatatgtat gagcccaacc gggacctgct
     4201 ggccagccac agcgcggagg acgaggccga ggacagtgcc atgtcgccca tccccgtggg
     4261 gccaccgtcc cccttcccca ccagcgagga cttcaccccc aaggagggct cgccgtacga
     4321 ggcccctgtc tacattcctg aagacattcc gatcccagca gacttcgagc tccgagagtc
     4381 ctccatccca ggggctggcc tgggggtctg ggccaagagg aagatggaag ccggggagag
     4441 gctgggcccc tgcgtggtgg tgccccgggc ggcggcaaag gagacagact tcggatggga
     4501 gcaaatactg acggacgtgg aagtgtcgcc ccaggaaggc tgcatcacaa agatctccga
     4561 agacctgggc agtgagaagt tctgcgtgga tgcaaatcag gcgggggctg gcagctggct
     4621 caagtacatc cgtgtggcgt gctcctgcga tgaccagaac ctcaccatgt gtcagatcag
     4681 tgagcagatt tactataaag tcattaagga cattgagcca ggtgaggagc tgctggtgca
     4741 cgtgaaggaa ggcgtctacc ccctgggcac agtgccgccc ggcctggacg aggagcccac
     4801 gttccgctgt gacgagtgtg acgaactctt ccagtccaag ctggacctgc ggcgccataa
     4861 gaagtacacg tgtggctcag tgggggctgc gctctacgag ggcctggctg aggagctcaa
     4921 gcccgagggc cttggcggtg gcagcggcca agcccacgag tgcaaggact gcgagcggat
     4981 gttccccaac aagtacagcc tggagcagca catggtcatc cacacggagg agcgcgagta
     5041 caaatgcgac cagtgtccca aggccttcaa ctggaagtcc aacctcatcc gccaccagat
     5101 gtcccacgac agcggcaaac gcttcgaatg tgaaaactgc gtgaaggtgt tcacggaccc
     5161 cagcaacctt cagcggcaca tccgctcgca gcacgtgggc gctcgggccc acgcctgccc
     5221 cgactgcggg aagaccttcg ccacgtcctc cggcctcaag cagcacaagc atatccacag
     5281 cacggtgaag cctttcatat gtgaggtctg ccacaagtcc tacacgcagt tctccaacct
     5341 gtgccggcac aagcggatgc acgccgactg ccgcacgcag atcaagtgca aggactgtgg
     5401 ccagatgttc agcactacct cctccctcaa caagcaccgg cgcttctgcg agggcaagaa
     5461 ccattacacg ccgggcggca tctttgcccc gggcctgccc ttgaccccca gccccatgat
     5521 ggacaaggca aaaccctccc ccagcctcaa tcacgccagc ctgggcttca acgagtactt
     5581 tccctccagg ccgcacccgg ggagcctgcc cttctccacg gcgcctccca cgttccccgc
     5641 actcaccccc ggcttcccgg gcatcttccc tccatccttg tacccccggc cgcctctgct
     5701 acctcccaca tcgctgctca agagccccct gaaccacacc caggacgcca agctccccag
     5761 tcccctgggg aacccagccc tgcccctggt ctccgccgtc agcaacagca gccagggcac
     5821 gacggcagct gcggggcccg aggagaagtt cgagagccgc ctggaggact cctgtgtgga
     5881 gaagctgaag accaggagca gcgacatgtc ggacggcagt gactttgagg acgtcaacac
     5941 caccacgggg accgacctgg acacgaccac ggggacgggc tcggacctgg acagcgacgt
     6001 ggacagcgac cctgacaagg acaagggcaa gggcaagtcc gccgagggcc agcccaagtt
     6061 tgggggcggc ttggcgcccc cgggggcccc gaacagcgtg gccgaggtgc ctgtcttcta
     6121 ttcccagcac tcattcttcc cgccacccga cgagcagctg ctgactgcaa cgggcgccgc
     6181 cggggactcc atcaaggcca tcgcatccat tgccgagaag tactttggcc ccggcttcat
     6241 ggggatgcag gagaagaagc tgggctcgct cccctaccac tcggcgttcc ccttccagtt
     6301 cctgcccaac ttcccccact ccctttaccc cttcacggac cgagccctcg cccacaactt
     6361 gctggtcaag gccgagccaa agtcaccccg ggacgccctc aaggtgggcg gccccagtgc
     6421 cgagtgcccc tttgatctca ccaccaagcc caaagacgtg aagcccatcc tgcccatgcc
     6481 caagggcccc tcggcccccg catccggcga ggagcagccg ctggacctga gcatcggcag
     6541 ccgggcccgt gccagccaaa acggcggcgg gcgggagccc cgcaagaacc acgtctatgg
     6601 ggaacgcaag ctgggcgccg gcgaggggct gccccaggtg tgcccggcgc ggatgcccca
     6661 gcagcccccg ctccactacg ccaagccctc gcccttcttc atggacccca tctacagcag
     6721 ggtagaaaag cggaaggtca cagaccccgt gggagccctg aaggagaagt acctgcggcc
     6781 gtccccgctg ctcttccacc cccagatgtc agccatagag accatgacag agaagctgga
     6841 gagctttgca gccatgaagg cggactcggg cagctccctg cagcccctcc cccaccaccc
     6901 cttcaacttc cggtccccac ccccaacgct ctccgacccc atcctcagga agggcaagga
     6961 gcgatacacg tgcaggtact gtgggaagat cttccccaga tcagccaatc tcaccagaca
     7021 cctgaggacg cacactgggg agcagccgta caggtgtaag tactgcgacc gctccttcag
     7081 catctcttcg aacctccagc ggcacgtccg gaacatccac aacaaggaga agcctttcaa
     7141 gtgccacctg tgcaaccgct gcttcgggca gcagaccaac ctggaccggc acctcaagaa
     7201 gcacgagcac gagaacgcac cagtgagcca gcaccccggg gtcctcacga accacctggg
     7261 gaccagcgcg tcctctccca cctcagagtc ggacaaccac gcacttttag acgagaaaga
     7321 agactcttat ttctcggaaa tcagaaactt tattgccaat agtgagatga accaagcatc
     7381 aacgcgaaca gagaaacggg cggacatgca gatcgtggac ggcagtgccc agtgtccagg
     7441 cctagccagt gagaagcagg aggacgtgga ggaggaggac gacgatgacc tggaggagga
     7501 cgatgaggac agcctggccg ggaagtcgca ggatgacacc gtgtcccccg cacccgagcc
     7561 ccaggccgcc tacgaggatg aggaggatga ggagccagcc gcctccctgg ccgtgggctt
     7621 tgaccacacc cgaaggtgtg ctgaggacca cgaaggcggt ctgttagctt tggagccgat
     7681 gccgactttt gggaaggggc tggacctccg cagagcagct gaggaagcat ttgaagttaa
     7741 agatgtgctt aattccacct tagattctga ggctttaaaa catacactgt gcaggcaggc
     7801 taagaaccag ggttctctgg acgcttggtt gaaggtcact ggagccacgt cggagtctgg
     7861 agcatttcac cccatcaacc acctctagta atgaactagt ccgtaacttg aaagtatttc
     7921 gatttcttgg ctttatatat cttgtggaaa ggacgataga tattaagcaa cgggctgtta
     7981 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     8041 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     8101 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     8161 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     8221 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     8281 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     8341 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     8401 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     8461 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     8521 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     8581 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8641 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     8701 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8761 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8821 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8881 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     8941 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     9001 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9061 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     9121 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9181 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     9241 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     9301 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     9361 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     9421 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     9481 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     9541 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     9601 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     9661 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     9721 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     9781 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     9841 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     9901 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     9961 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    10021 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    10081 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    10141 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    10201 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    10261 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
    10321 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    10381 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    10441 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    10501 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    10561 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    10621 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    10681 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    10741 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    10801 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    10861 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    10921 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10981 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    11041 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    11101 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    11161 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    11221 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    11281 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    11341 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    11401 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    11461 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    11521 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    11581 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    11641 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    11701 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    11761 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    11821 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    11881 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    11941 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    12001 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    12061 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
    12121 aaaagctgga gctgcaagc
//
