LOCUS       sequence-39770-e        8853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Murin CD, Segal K, Bryksin A, Matsumura I
  TITLE     Expression vectors for the engineering of genes and genomes in 
            Acinetobacter baylyi ADP1.
  JOURNAL   Appl Environ Microbiol. 2011 Oct 21.
  PUBMED    22020504
REFERENCE   2  (bases 1 to 8853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2011 Oct 21."
COMMENT     Sequence Label: pp2.1-PT5-lacI-gusA-specR-pp2.2-IMBB
FEATURES             Location/Qualifiers
     source          1..8853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1081..1095,1096..1101,1102..1118,1119..1125)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    1101..1117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1133..1149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1172..1183
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1202..1219
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1244..2323
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(1263..1282)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             2386..2403
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2428..4233
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA
                     VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM
                     EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF
                     FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV
                     VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFL
                     INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA
                     DEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
                     NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        4273..4364
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4365..5156
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      complement(5327..5374)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     5370..5388
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(5426..5470)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     misc_feature    6522..6542
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     rep_origin      complement(6604..7192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6684..6703)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7363..8154,8155..8223))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7986..8005
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8224..8328)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8396..8414
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8452..8474)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8574..8593
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8747..8768)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(8838..8853,1..4)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 ctagatgtta tttcttcaaa attatggatt aatttaaatt gttgagccga cattttatta
       61 ccctcttatc aaaccgtacc tttcacataa cgaatgaatg aataccgtac atggagtgcg
      121 gccaacccac agcgaacatc atatttcgca tccatcaccg tacggttttc cgttttaagc
      181 tctgcccatg atctatcatg gaaataacgg ctaatgatca cctgcatcca ctcaagtgtc
      241 gtttcactgt ctgtaccatt aataatatcc agtactaaac gttgtacggc acgagcttca
      301 ttatcgttaa tctgacacga cactttgtga cgtatagctt gttgtactcc ttgagcatca
      361 caaaggtaat aagcaataag tttagctcga tctttcttct ttacacgtac cttggctttc
      421 ttcattgcaa tagcaatcgg gctatcggaa tactgtccac cacgacaaga acgttgccac
      481 gctccacatt ggcgtaacca atctggcaaa tcatacttgc tccaatccac cgtctgcata
      541 atgtgcactg ctgtattcat ctcatcacct aatttgtttc aagttaaatt ttataagcgt
      601 tattgtttta tggttctgcc tgctcctcta ccgatctaaa acgacaagtt tcgagataat
      661 ccagtactcg aactgcaccg cgtttaccgt gtcggttttt cactacaatc agctctgtga
      721 ttcccatcgg tttggttgag tcttctggat cggttagggg gttaacaagg atgatctggt
      781 ctgcatcttg ctcgatttgt ccagattctt tgatatctga tgctttagga cgtttgccct
      841 tctctgcctc acggttgagc tgtaccagtg caatgacagg acattcaaac tctttcgcca
      901 tggattttaa ttcacggctg atggaactga cttcctgaaa gcgatctttc ttgctcgggt
      961 ctctgagcag ttgtaaataa tccacgatga tgcagcccaa ttccttgtaa cggcgtttgg
     1021 ctcgacgtgc ataggaacgg acctcactca agcttactag aactatagct agtcgagaaa
     1081 tcataaaaaa tttatttgct ttgtgagcgg ataacaatta taatagattc aattgtgagc
     1141 ggataacaat ttcacacaga attaattcat taaagaggag aaattaacca tgggcagcag
     1201 ccatcaccat catcaccaca gccaggatcc ggccctgagg gccgtgaaac cagtaacgtt
     1261 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
     1321 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
     1381 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
     1441 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
     1501 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
     1561 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
     1621 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
     1681 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
     1741 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
     1801 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
     1861 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
     1921 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
     1981 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
     2041 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
     2101 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
     2161 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
     2221 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2281 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagggcctat gcggccggta
     2341 gaaaggagaa gcttactaga gcatgcggag accatgggca gcagccatca ccatcatcac
     2401 cacagccagg atccggccct gagggcctta cgtcctgtag aaaccccaac ccgtgaaatc
     2461 aaaaaactcg acggcctgtg ggcattcagt ctggatcgcg aaaactgtgg aattgatcag
     2521 cgttggtggg aaagcgcgtt acaagaaagc cgggcaattg ctgtgccagg cagttttaac
     2581 gatcagttcg ccgatgcaga tattcgtaat tatgcgggca acgtctggta tcagcgcgaa
     2641 gtctttatac cgaaaggttg ggcaggccag cgtatcgtgc tgcgtttcga tgcggtcact
     2701 cattacggca aagtgtgggt caataatcag gaagtgatgg agcatcaggg cggctatacg
     2761 ccatttgaag ccgatgtcac gccgtatgtt attgccggga aaagtgtacg tatcaccgtt
     2821 tgtgtgaaca acgaactgaa ctggcagact atcccgccgg gaatggtgat taccgacgaa
     2881 aacggcaaga aaaagcagtc ttacttccat gatttcttta actatgccgg aatccatcgc
     2941 agcgtaatgc tctacaccac gccgaacacc tgggtggacg atatcaccgt ggtgacgcat
     3001 gtcgcgcaag actgtaacca cgcgtctgtt gactggcagg tggtggccaa tggtgatgtc
     3061 agcgttgaac tgcgtgatgc ggatcaacag gtggttgcaa ctggacaagg cactagcggg
     3121 actttgcaag tggtgaatcc gcacctctgg caaccgggtg aaggttatct ctatgaactg
     3181 tgcgtcacag ccaaaagcca gacagagtgt gatatctacc cgcttcgcgt cggcatccgg
     3241 tcagtggcag tgaagggcga acagttcctg attaaccaca aaccgttcta ctttactggc
     3301 tttggtcgtc atgaagatgc ggacttgcgt ggcaaaggat tcgataacgt gctgatggtg
     3361 cacgaccacg cattaatgga ctggattggg gccaactcct accgtacctc gcattaccct
     3421 tacgctgaag agatgctcga ctgggcagat gaacatggca tcgtggtgat tgatgaaact
     3481 gctgctgtcg gctttaacct ctctttaggc attggtttcg aagcgggcaa caagccgaaa
     3541 gaactgtaca gcgaagaggc agtcaacggg gaaactcagc aagcgcactt acaggcgatt
     3601 aaagagctga tagcgcgtga caaaaaccac ccaagcgtgg tgatgtggag tattgccaac
     3661 gaaccggata cccgtccgca aggtgcacgg gaatatttcg cgccactggc ggaagcaacg
     3721 cgtaaactcg acccgacgcg tccgatcacc tgcgtcaatg taatgttctg cgacgctcac
     3781 accgatacca tcagcgatct ctttgatgtg ctgtgcctga accgttatta cggatggtat
     3841 gtccaaagcg gcgatttgga aacggcagag aaggtactgg aaaaagaact tctggcctgg
     3901 caggagaaac tgcatcagcc gattatcatc accgaatacg gcgtggatac gttagccggg
     3961 ctgcactcaa tgtacaccga catgtggagt gaagagtatc agtgtgcatg gctggatatg
     4021 tatcaccgcg tctttgatcg cgtcagcgcc gtcgtcggtg aacaggtatg gaatttcgcc
     4081 gattttgcga cctcgcaagg catattgcgc gttggcggta acaagaaagg gatcttcact
     4141 cgcgaccgca aaccgaagtc ggcggctttt ctgctgcaaa aacgctggac tggcatgaac
     4201 ttcggtgaaa aaccgcagca gggaggcaaa caaggcctat gcggccggta gaaaggagaa
     4261 gcttactagc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4321 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagg gaagcggtga
     4381 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     4441 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     4501 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     4561 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     4621 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     4681 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     4741 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     4801 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     4861 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     4921 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     4981 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     5041 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     5101 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc
     5161 taacaattcg ttcaagccga ggggccgcaa gatccggcca cgatgacccg gtcgtcggtt
     5221 cagggcaggg tcgttaaata gccgcttatg tctattgctg gtttaccggt ttattgacta
     5281 ccggaagcag tgtgaccgtg tgcttctcaa atgcctgagg tttcagcaaa aaacccctca
     5341 agacccgttt agaggcccca aggggttatg ctagttattg ctcagcggtg gcagcagcct
     5401 aggttaatta agctgcgcta gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag
     5461 cggtttcttt accagactcg acaagcttac tagagtgttc atattgacct cgcttagtgt
     5521 ggttaatacg ccgcttcttg ttactgcaag aggcggtttt tttatgggtg tacacatgac
     5581 tgcacctgtt gattcagttc agatagtgct ttgtgcatct catggatgac tctggccata
     5641 tccagtgctt cgccttgggt aattcgccca tcggccatca tttctttaaa cagtgctgat
     5701 atatcgccct tcttgatgcc tatgcatagg aaggtatcca tcagactggt atctcgctgg
     5761 ctctcgggta tgtctggcag gtcaattgcc acctttccga gtcgtgcaca catttcctgc
     5821 aatatccgat agtcccctgt aatctccatc agcttgactg cctcgagcaa tgtaatgtga
     5881 tgggtatgtg tgtttgggtt gaccttgcta ttgagcaccg cagggctttt gatgcctaaa
     5941 cgtgatgcaa gtgcagatgc accacccaga aagtcgtgaa cggtgtgata ggcagcatct
     6001 aatatgttca tggcgggttc ctttgaacgt gtttattaga tgggtgctga cataagattg
     6061 gatttatggt taggacgtaa ttcaatccaa atatcttcgt aatcatctgg aaataaatct
     6121 ttgcgagtgc atagaccttt atcttcagca attacagcta agcggatttt gcgatctctg
     6181 ggaattgctt tccatccact tacggatgca gcggtgatac ctaaaaatct agcaacagca
     6241 gttacaccac ctaaaagctc aataaattga tcatcagtca tgttgatctc ctaattttat
     6301 tgcctcaatt attaggtatt ccttatattt tatcaatagg aataccttat ttattttatg
     6361 ttaggatttc ctaatagact aggtaagatc atgaaaacat tagctgaacg acttaaatat
     6421 gcgatggaaa ttttgccacc taagaaaatc aagggtgtcg aacttgctcg tgtagttgga
     6481 gttaaaccac catctgtcag cgattggctt tcaggtaagc ttactagtag cggccgctgc
     6541 agacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6601 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6661 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6721 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6781 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6841 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6901 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6961 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7021 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7081 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7141 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7201 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7261 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7321 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7381 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7441 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7501 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7561 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7621 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7681 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7741 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7801 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7861 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7921 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     7981 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8041 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8101 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8161 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8221 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8281 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8341 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     8401 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     8461 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     8521 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     8581 agagcagatt gtactgagag tgcaccataa aattgtaaac gttaatattt tgttaaaatt
     8641 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     8701 cccttataaa tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa
     8761 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     8821 cgatggccca ctacgtggaa ttcgcggccg ctt
//
