LOCUS       sequence-397717-       12505 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12505)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2774
FEATURES             Location/Qualifiers
     source          1..12505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    4913..4924
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     misc_feature    8362..8950
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8415..8435)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8833..8844)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9022..9255
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9333..9454
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9370..9389)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9424..9443
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9494..9629
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9649..9668)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9650..9668)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9678..9695)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9678..9694)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9687..9709)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9836..10291
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9923..9942)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10133..10154
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10317..10421
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10422..10490,10491..11282)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10640..10659)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11453..12041
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11942..11961
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12195..12212
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12329..12350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12365..12370,12371..12388,12389..12395)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12403..12419
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12408..12430
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12427..12443
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12427..12443
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12462..12482
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12464..12482
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag ccggggtccg acgatttcct
     4141 accgccaccg gagtgcccgg tgttcgagcc tagctgggcc gagttccgag accctcttgg
     4201 ctacatcgcg aaaatcaggc ccatcgcaga gaaatcgggc atttgcaaga tccgcccacc
     4261 cgcgattgtg gtggaggaag gtggttatga agctatctgc aaggaccgtc ggtgggctcg
     4321 ggtagcccag cgcctcaact atccaccagg caaaaatatt ggctccttgc tacgctccca
     4381 ctacgaacgc attgtttatc cctatgaaat gtaccagtct ggagccaacc ttgtgcagtg
     4441 taacacacgt ccatttgata atgaggagaa ggacaaggaa tacaaacccc acagcatccc
     4501 cctacgacag tctgtgcagc cttccaagtt caacagctat ggccggcggg ccaagagact
     4561 gcagcctgat ccggaaccca cagaggaaga cattgagaag aatccagagc tgaaaaagct
     4621 acagatctat ggggcaggcc ccaagatgat gggcctgggc ctcatggcca aagacaagac
     4681 tctgcggaag aaagataagg aggggcctga gtgtcccccc acagtagtgg tgaaggagga
     4741 gttaggtggg gatgtgaagg tggagtcaac atcgcctaag accttcctgg agagcaagga
     4801 ggagctgagt cacagcccag aaccctgcac caagatgacc atgaggctac ggaggaacca
     4861 cagcaatgcc cagtttattg agtcatatgt ctgccggatg tgttctcgag gggatgagga
     4921 tgacaagctc ctgctgtgtg atggctgtga tgacaactac cacatcttct gcctgctgcc
     4981 tcctctgcct gagatcccca agggtgtctg gcggtgccca aagtgtgtca tggcggagtg
     5041 taagcggccc ccagaagcct ttggctttga gcaggctacc cgggaataca ctctgcagag
     5101 ctttggcgag atggccgact cctttaaagc tgactacttc aacatgcccg tgcatatggt
     5161 gcccacagaa cttgtggaga aggagttctg gaggctggta aatagcattg aggaagatgt
     5221 gactgttgag tatggagctg acatccattc caaagaattt ggcagcggtt tccctgtcag
     5281 tgacagtaaa cggcacctaa cccccgaaga ggaggagtat gctaccagtg gttggaacct
     5341 aaatgtgatg ccggtgttgg aacagtctgt actgtgccac atcaatgcag atatctctgg
     5401 catgaaggtg ccctggctct acgtgggcat ggtcttctca gccttttgct ggcatattga
     5461 ggatcactgg agttactcca ttaactacct ccactggggt gagccgaaga cctggtatgg
     5521 ggtgccctca cttgcagcag aacatttgga agaagtgatg aagaagctga cacctgaact
     5581 atttgatagc cagcctgacc tcctgcacca acttgtcacc ctcatgaatc ccaacaccct
     5641 catgtcccat ggtgtgccag ttgtccgcac aaaccagtgt gcaggagagt ttgtcatcac
     5701 cttcccccgt gcttaccaca gcggcttcaa ccaaggctac aactttgccg aggctgtcaa
     5761 cttttgcact gctgactggt tgcctgctgg gcgccagtgc attgagcact accgccggct
     5821 ccggagatac tgcgtcttct cccatgagga gcttatctgc aagatggctg cctgcccaga
     5881 gaagctagac ctgaacctgg cggcagctgt gcataaggag atgttcatca tggtgcaaga
     5941 agagcggcgt ctacgaaagg ccctgctgga gaagggtatc acagaggctg agcgagaggc
     6001 tttcgagctg ctcccagatg atgagcgcca gtgtatcaag tgcaagacta cgtgtttcct
     6061 gtcagccctg gcctgctacg actgcccaga cggccttgtc tgcctttccc acatcaatga
     6121 tctctgcaag tgctccagta gccggcagta cctgcggtat cggtatacct tggatgagct
     6181 tcctgccatg ctgcataagc tgaaggttcg ggctgagtcc tttgacacct gggccaacaa
     6241 agtgcgagtg gccctggagg tggaggatgg gcggaagcgc agccttgaag aactgagggc
     6301 actagagtct gaagcccgtg agcggaggtt tcctaatagt gagctgctgc agcaactaaa
     6361 gaactgcctg agtgaggcag aggcttgcgt gtcccgagct ctgggactgg tcagcggcca
     6421 ggaagctggc ccccacaggg tggctggtct acagatgacc ctgactgagc tccgggcctt
     6481 tctggaccag atgaacaacc tgccttgcgc catgcaccag attggggatg tcaagggtgt
     6541 tctggaacag gtggaggcct accaggctga ggctcgtgag gccctggcct cactgccctc
     6601 cagtccaggg ctactgcagt ccctgttgga gagggggcgg cagctggggg tggaggtgcc
     6661 tgaggcccag cagctccagc ggcaggtgga acaggcgcga tggctggatg aggtgaaacg
     6721 cacactggcc ccctcagccc gaaggggcac cttggctgtc atgcgaggac tgttggtcgc
     6781 gggtgccagt gtagccccta gccctgctgt ggataaagcc caggccgagc tgcaggaact
     6841 gctgaccatt gctgaacgct gggaggagaa agcccacctc tgcctggagg ccaggcagaa
     6901 gcatccacca gccacacttg aggccataat ccgtgaagcg gaaaacatcc ctgttcacct
     6961 gcccaacatc caggctctca aggaggctct tgctaaggcc cgggcctgga ttgctgatgt
     7021 tgatgagatc caaaatggtg accactaccc ctgcctggat gacttggagg gcctagtagc
     7081 tgtgggccgg gacctacctg tggggctgga ggagctgaga cagctagagc tacaggtact
     7141 gacagcgcac tcctggaggg agaaggcctc caagaccttc ctcaagaaaa attcttgcta
     7201 cacgctgctg gaggttctct gcccatgtgc agatgccggc tcagacagca ccaagcgcag
     7261 ccggtggatg gagaaggagc tggggttgta caaatctgac acagagctgc tggggctgtc
     7321 tgcgcaggac ctcagggacc caggctctgt gatcgtggcc ttcaaggagg gggaacagaa
     7381 ggagaaggag ggtatcctgc agctgcgtcg caccaattcg gccaagccca gtccactggc
     7441 atcatcgagc acggcctcct ctacaacctc tatctgtgtg tgtgggcagg tgctggctgg
     7501 ggcgggagct ctgcagtgtg acctgtgtca ggactggttc catgggcggt gtgtgtcagt
     7561 gcctcgcctc ctcagctctc cgaggcccaa tcccacctca tccccactgc tggcctggtg
     7621 ggaatgggac accaaattcc tgtgtccact gtgtatgcgc tcaaggcgcc cgcgcctgga
     7681 gaccatcctg gcactgctgg tagccctgca gagactgcct gtgcggctgc ccgagggcga
     7741 ggccctgcag tgcctcacag agagggccat cagctggcaa ggccgcgcca ggcaggctct
     7801 ggcctctgaa gatgtgactg ctcttttggg acggctggct gagctccgcc aacggctaca
     7861 ggctgaacct agacctgagg agcctcctaa ctaccctgca gcccctgctt ctgaccccct
     7921 cagagagggc agtggcaagg atatgcctaa ggtccagggc ttactggaga atggagacag
     7981 tgtgaccagt cctgagaagg tagccccgga ggagggctca gatctggagc tgctgtcctc
     8041 gctgttgcca cagttgactg gccctgtgtt ggaactgcct gaggcaaccc gggccccctt
     8101 ggaggagctc atgatggagg gggacctgct cgaggtgacc ctggatgaga accacagcat
     8161 ccctgagtcc cttgactttt gtattctgac tccaaggtat tgttcagacc tcagctcctg
     8221 ggggccggcc cctggagtct tccctccctg gtagtaatga actagtccgt aacttgaaag
     8281 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaatttgatc attaagttcc
     8341 ctcgacacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     8401 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     8461 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     8521 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     8581 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     8641 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     8701 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     8761 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     8821 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     8881 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     8941 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     9001 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     9061 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     9121 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     9181 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     9241 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     9301 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     9361 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9421 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     9481 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     9541 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     9601 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     9661 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     9721 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     9781 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     9841 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     9901 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     9961 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    10021 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    10081 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    10141 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    10201 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    10261 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
    10321 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    10381 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    10441 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    10501 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    10561 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    10621 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    10681 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    10741 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    10801 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    10861 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    10921 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    10981 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    11041 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    11101 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    11161 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    11221 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    11281 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    11341 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    11401 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    11461 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    11521 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    11581 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    11641 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    11701 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    11761 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    11821 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    11881 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    11941 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    12001 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    12061 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    12121 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    12181 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    12241 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    12301 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    12361 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    12421 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    12481 gggaacaaaa gctggagctg caagc
//
