LOCUS       sequence-397722-       10891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10891)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0074
FEATURES             Location/Qualifiers
     source          1..10891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6748..7336
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6801..6821)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7219..7230)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7408..7641
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7719..7840
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7756..7775)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7810..7829
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7880..8015
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8035..8054)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8036..8054)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8064..8081)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8064..8080)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8073..8095)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8222..8677
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8309..8328)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8519..8540
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8703..8807
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8808..8876,8877..9668)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9026..9045)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9839..10427
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10328..10347
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10581..10598
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10715..10736
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10751..10756,10757..10774,10775..10781)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10789..10805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10794..10816
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10813..10829
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10813..10829
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10848..10868
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10850..10868
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtct gtggatgaga agcctgactc
     4141 ccccatgtat gtgtatgagt ccacagtcca ctgcaccaac atcctcctgg gcctcaatga
     4201 ccagcggaaa aaggatattc tctgtgacgt gactttgatc gtggagagga aggagttccg
     4261 ggcccaccgg gctgtgctgg ccgcatgcag tgaatatttt tggcaggcgc tggttggaca
     4321 gacaaaaaat gatttggtgg tcagcttgcc tgaggaggtc acagccaggg gctttgggcc
     4381 gctgttacag tttgcctaca ctgccaagct gttactcagc agagaaaaca tccgcgaggt
     4441 catccgctgt gctgagttcc tgcgcatgca caacctggag gactcctgct tcagcttcct
     4501 gcagacccag ctcctgaaca gtgaggatgg cctgtttgtg tgccggaagg atgctgcgtg
     4561 ccagcgccca cacgaggact gcgagaactc tgcaggagag gaggaggatg aagaggagga
     4621 gacgatggat tcagagacgg ccaagatggc ttgccccagg gaccagatgc ttccagagcc
     4681 catcagcttt gaggccgccg ccatccccgt agcagagaag gaagaagccc tgctgcccga
     4741 gcctgacgtg cccacagaca ccaaggagag ctcagaaaag gacgcgttaa cgcagtaccc
     4801 cagatacaag aaataccagc ttgcatgtac caagaatgtc tataatgcat catcacacag
     4861 tacctcaggt tttgcaagca cattccggga agataactct agcaacagcc tcaagccggg
     4921 gcttgccagg gggcagatta aaagtgagcc gcccagtgaa gagaatgagg aagagagcat
     4981 cacgctctgc ctgtctggag atgagcctga cgccaaggac agagcggggg atgtcgagat
     5041 ggaccggaaa cagcccagcc ctgcccctac ccccacggcc ccagctgggg ccgcctgcct
     5101 ggagagatcc aggagcgtgg cctcgccctc ctgcttaagg tctctgttca gcataacgaa
     5161 aagtgtggag ctgtctggcc tgcccagtac atctcagcag cactttgcca ggagtccagc
     5221 ctgccctttt gacaagggga tcactcaggg tgaccttaaa actgactaca cccctttcac
     5281 agggaattat ggacagcccc acgtgggcca gaaggaggtg tccaacttca ccatggggtc
     5341 gcccctcagg gggcctgggt tggaggctct ctgtaaacag gagggagagc tggaccggag
     5401 gagcgtgatc ttctcctcca gcgcttgtga ccaagtgagc acctcggtgc attcttattc
     5461 tggggtgagc agtttggaca aagacctctc tgagccggtg ccaaagggtc tgtgggtggg
     5521 agccggccag tccctcccca gctcgcaggc ctactcccac ggtgggctga tggccgacca
     5581 cttgccagga aggatgcggc ccaacaccag ctgcccggta ccaatcaaag tctgccctcg
     5641 ctcacccccc ttggagacca ggaccaggac ttccagctcc tgctcttcct attcctacgc
     5701 ggaggacggg agcgggggct caccctgcag cctccctctc tgtgagttct cctcctcgcc
     5761 ctgttcccag ggagccagat tccttgccac agaacatcag gaaccaggcc tgatgggaga
     5821 tggaatgtac aaccaagtgc ggccccaaat taaatgtgag cagtcttatg gaaccaactc
     5881 cagtgacgaa tccggatcgt tctcggaagc agacagtgag tcgtgtcctg tgcaggacag
     5941 gggccaggag gtaaaacttc cttttcctgt agatcaaatc acagatcttc caaggaacga
     6001 tttccagatg atgattaaaa tgcacaagct aacctcagaa cagttagagt ttattcatga
     6061 tgtccgacgg cgcagcaaga accgcatcgc ggcccagcgc tgccgcaaaa ggaaactgga
     6121 ctgtattcag aatttagaat gtgaaatccg caaattggtg tgtgagaaag agaaactgtt
     6181 gtcagagagg aatcaactga aagcatgcat gggggaactg ttggacaact tctcctgcct
     6241 ttcccaggaa gtttgccgag acatccagag ccccgagcag atccaggccc tgcatcggta
     6301 ttgccctgtc ctcagaccca tggacttgcc cacggcctcc agtattaacc ctgcgccctt
     6361 gggtgctgag cagaacattg cggcctccca atgcgcagtg ggggaaaacg tgccctgctg
     6421 cttggagcca ggcgcggctc cccccggacc cccctgggca cccagcaaca cctccgagaa
     6481 ttgtacctct gggaggagac tagaaggcac tgacccggga accttctcag agagaggacc
     6541 tcctcttgaa cccaggagcc aaacagtgac cgtggacttc tgccaggaaa tgactgataa
     6601 gtgtacaact gacgaacagc ccaggaaaga ttatacctag taatgaacta gtccgtaact
     6661 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgacg gcagtgtcaa
     6721 ctaaggagga caacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     6781 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6841 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6901 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6961 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7021 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7081 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7141 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7201 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7261 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7321 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     7381 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     7441 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7501 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7561 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7621 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     7681 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     7741 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7801 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     7861 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7921 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7981 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     8041 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     8101 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     8161 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     8221 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8281 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8341 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8401 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8461 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8521 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8581 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8641 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     8701 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8761 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8821 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8881 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8941 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     9001 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     9061 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     9121 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     9181 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     9241 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     9301 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     9361 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     9421 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9481 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9541 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9601 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9661 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9721 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9781 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9841 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9901 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9961 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    10021 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    10081 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    10141 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10201 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    10261 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10321 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10381 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    10441 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    10501 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    10561 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    10621 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    10681 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    10741 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    10801 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    10861 actaaaggga acaaaagctg gagctgcaag c
//
