LOCUS       sequence-39776-e        6048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Murin CD, Segal K, Bryksin A, Matsumura I
  TITLE     Expression vectors for the engineering of genes and genomes in 
            Acinetobacter baylyi ADP1.
  JOURNAL   Appl Environ Microbiol. 2011 Oct 21.
  PUBMED    22020504
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2011 Oct 21."
COMMENT     Sequence Label: araC-pBAD-gusA-pBAV1k
FEATURES             Location/Qualifiers
     source          1..6048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             331..348
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             373..2178
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA
                     VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM
                     EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF
                     FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV
                     VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFL
                     INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA
                     DEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
                     NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     misc_feature    2209..2229
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      2280..2366
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             3697..3708
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(3910..4004)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4020..4814)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEEYRCVKDTEGMSPAKVYKLVGENEILYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEGYED
                     EQSPEKTIELYAECIRLFHSIDISDCPYTNSLDGRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    5020..5039
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(5144..6022)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat gggcagcagc catcaccatc atcaccacag ccaggatccg
      361 gccctgaggg ccttacgtcc tgtagaaacc ccaacccgtg aaatcaaaaa actcgacggc
      421 ctgtgggcat tcagtctgga tcgcgaaaac tgtggaattg atcagcgttg gtgggaaagc
      481 gcgttacaag aaagccgggc aattgctgtg ccaggcagtt ttaacgatca gttcgccgat
      541 gcagatattc gtaattatgc gggcaacgtc tggtatcagc gcgaagtctt tataccgaaa
      601 ggttgggcag gccagcgtat cgtgctgcgt ttcgatgcgg tcactcatta cggcaaagtg
      661 tgggtcaata atcaggaagt gatggagcat cagggcggct atacgccatt tgaagccgat
      721 gtcacgccgt atgttattgc cgggaaaagt gtacgtatca ccgtttgtgt gaacaacgaa
      781 ctgaactggc agactatccc gccgggaatg gtgattaccg acgaaaacgg caagaaaaag
      841 cagtcttact tccatgattt ctttaactat gccggaatcc atcgcagcgt aatgctctac
      901 accacgccga acacctgggt ggacgatatc accgtggtga cgcatgtcgc gcaagactgt
      961 aaccacgcgt ctgttgactg gcaggtggtg gccaatggtg atgtcagcgt tgaactgcgt
     1021 gatgcggatc aacaggtggt tgcaactgga caaggcacta gcgggacttt gcaagtggtg
     1081 aatccgcacc tctggcaacc gggtgaaggt tatctctatg aactgtgcgt cacagccaaa
     1141 agccagacag agtgtgatat ctacccgctt cgcgtcggca tccggtcagt ggcagtgaag
     1201 ggcgaacagt tcctgattaa ccacaaaccg ttctacttta ctggctttgg tcgtcatgaa
     1261 gatgcggact tgcgtggcaa aggattcgat aacgtgctga tggtgcacga ccacgcatta
     1321 atggactgga ttggggccaa ctcctaccgt acctcgcatt acccttacgc tgaagagatg
     1381 ctcgactggg cagatgaaca tggcatcgtg gtgattgatg aaactgctgc tgtcggcttt
     1441 aacctctctt taggcattgg tttcgaagcg ggcaacaagc cgaaagaact gtacagcgaa
     1501 gaggcagtca acggggaaac tcagcaagcg cacttacagg cgattaaaga gctgatagcg
     1561 cgtgacaaaa accacccaag cgtggtgatg tggagtattg ccaacgaacc ggatacccgt
     1621 ccgcaaggtg cacgggaata tttcgcgcca ctggcggaag caacgcgtaa actcgacccg
     1681 acgcgtccga tcacctgcgt caatgtaatg ttctgcgacg ctcacaccga taccatcagc
     1741 gatctctttg atgtgctgtg cctgaaccgt tattacggat ggtatgtcca aagcggcgat
     1801 ttggaaacgg cagagaaggt actggaaaaa gaacttctgg cctggcagga gaaactgcat
     1861 cagccgatta tcatcaccga atacggcgtg gatacgttag ccgggctgca ctcaatgtac
     1921 accgacatgt ggagtgaaga gtatcagtgt gcatggctgg atatgtatca ccgcgtcttt
     1981 gatcgcgtca gcgccgtcgt cggtgaacag gtatggaatt tcgccgattt tgcgacctcg
     2041 caaggcatat tgcgcgttgg cggtaacaag aaagggatct tcactcgcga ccgcaaaccg
     2101 aagtcggcgg cttttctgct gcaaaaacgc tggactggca tgaacttcgg tgaaaaaccg
     2161 cagcagggag gcaaacaagg cctatgcggc cggtagaaag gagaagctta ctagtagcgg
     2221 ccgctgcagg cctcagggcc cgatcgatgc cgccgcttaa ttaattaatc cagaggcatc
     2281 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2341 tgaacgctct cctgagtagg acaaatccgc cgccctagac ctagtgtcat tttatttccc
     2401 ccgtttcagc atcaagaacc tttgcataac ttgctctata tccacactga taattgccct
     2461 caaaccataa tctaaaggcg ctagagtttg ttgaaacaat atcttttaca tcattcgtat
     2521 ttaaaattcc aaactccgct cccctaaggc gaataaaagc cattaaatct tttgtattta
     2581 ccaaattata gtcatccact atatctaaga gtaaattctt caattctctt ttttggcttt
     2641 catcaagtgt tatatagcgg tcaatatcaa aatcattaat gttcaaaata tcttttttgt
     2701 cgtatatatg tttattctta gcaatagcgt cctttgattc atgagtcaaa tattcatatg
     2761 aacctttgat ataatcaagt atctcaacat gagcaactga actattcccc aattttcgct
     2821 taatcttgtt cctaacgctt tctattgtta caggatttcg tgcaatatat ataacgtgat
     2881 agtgtggttt tttatagtgc tttccatttc gtataacatc actactattc catgtatctt
     2941 tatctttttt ttcgtccata tcgtgtaaag gactgacagc catagatacg cccaaactct
     3001 ctaatttttc cttccaatca ttaggaattg agtcaggata taataaaaat ccaaaatttc
     3061 tagctttagt atttttaata gccatgatat aattacctta tcaaaaacaa gtagcgaaaa
     3121 ctcgtatcct tctaaaaacg cgagctttcg cttatttttt ttgttctgat tcctttcttg
     3181 catattcttc tatagctaac gccgcaaccg cagattttga aaaacctttt tgtttcgcca
     3241 tatctgttaa ttttttatct tgctcttttg tcagagaaat cataactctt tttttcgatt
     3301 ctgaaatcac catttaaaaa actccaatca aataatttta taaagttagt gtatcacttt
     3361 gtaatcataa aaacaacaat aaagctactt aaatatagat ttataaaaaa cgttggcgaa
     3421 aacgttggcg attcgttggc gattgaaaaa ccccttaaac ccttgagcca gttgggatag
     3481 agcgtttttg gcacaaaaat tggcactcgg cacttaatgg ggggtcgtag tacggaagca
     3541 aaattcgctt cctttccccc catttttttc caaattccaa atttttttca aaaattttcc
     3601 agcgctaccg ctcggcaaaa ttgcaagcaa tttttaaaat caaacccatg agggaatttc
     3661 attccctcat actcccttga gcctcctcca accgaaatag aagggcgctg cgcttattat
     3721 ttcattcagt catcggcttt cataatctaa cagacaacat cttcgctgca aagccacgct
     3781 acgctcaagg gcttttacgc tacgataacg cctgttttaa cgattatgcc gataactaaa
     3841 cgaaataaac gctaaaacgt ctcagaaacg attttgagac gttttaataa aaaatcgcct
     3901 agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     3961 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcggtac
     4021 taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc
     4081 agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg
     4141 acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag
     4201 ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag
     4261 acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta
     4321 aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag
     4381 taatccaatt cggctaagcg gccgtctaag ctattcgtat agggacaatc cgatatgtcg
     4441 atggagtgaa agagcctgat gcactccgca tacagctcga tagtcttttc agggctttgt
     4501 tcatcttcat acccttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc
     4561 cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat
     4621 agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc
     4681 atttttaaat ataggatttc attttctccc accagcttat ataccttagc aggagacatt
     4741 ccttccgtat cttttacgca gcggtattct tcgatcagtt ttttcaattc cggtgatatt
     4801 ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag
     4861 aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat
     4921 acagaaaaca gccttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga
     4981 gccgattttg aaaccacaat tatgatagaa tttgacgtcg aattcgcggc cgcttctaga
     5041 actatagcta gcatgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     5101 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     5161 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     5221 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     5281 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct
     5341 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     5401 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     5461 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt
     5521 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     5581 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     5641 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa
     5701 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     5761 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     5821 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     5881 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     5941 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg
     6001 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagag
//
