LOCUS       sequence-39777-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Calvisi DF, Wang C, Ho C, Ladu S, Lee SA, Mattu S, Destefanis G, 
            Delogu S, Zimmermann A, Ericsson J, Brozzetti S, Staniscia T, Chen 
            X, Dombrowski F, Evert M
  TITLE     Increased lipogenesis, induced by AKT-mTORC1-RPS6 signaling, 
            promotes development of human hepatocellular carcinoma.
  JOURNAL   Gastroenterology. 2011 Mar;140(3):1071-83. Epub 2010 Dec 11.
  PUBMED    21147110
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Gastroenterology."; date: "2011-03"; volume: "140(3)"; pages: 
            "1071-83. Epub 2010 Dec 11"
COMMENT     Sequence Label: pT3-myr-AKT-HA
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..85
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(123..164)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    217..241
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(261..287)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
       61 tggcaactag aaggcacagt cgaggctgat cagcgggttt aaactcatta ctaaccggta
      121 cgcgtagaat cgagaccgag gagagggtta gggataggct taccttcgaa ccgcgggccc
      181 tctagactcg agcggccgcc actgtgctgg atatcaacca ctttgtacaa gaaagctggg
      241 tcggcgcgcc caccctttca agcgtagtct gggacgtcgt atgggtaggc tgtgccactg
      301 gctgagtagg agaactgggg gaagtgcggc ctccgctcac tgtccacaca ctccatgctg
      361 tcatcttgat caggcggcgt gatggtgatc atctgagctg tgaactcctc atcgaaatac
      421 ctggtgtcag tctcagaggt gacctggggc ttgaaaggtg ggctcagctt cttctcatac
      481 acatcctgcc acacgatgtt ggcaaagaac cggtgctgca tgatctcctt ggcatcctca
      541 gagcccccac cgagcctctg tgtagggtcc ttcttgagca gcccggagag cagggacttg
      601 gcctcagggc cgagtgtgcg cgggaagcgg atctcctcca tgaggatcag ctcgaacagc
      661 ttctcgtggt cctggttgta gaagggcagg cggccacaca tcatctcata catgaccacg
      721 cccagccccc accagtccac tgcacggccg tagtcgttgt cctccagcac ctcaggggcc
      781 aggtactccg gcgttccgca gaatgtc
//
