LOCUS       sequence-397837-       10768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10768)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2800
FEATURES             Location/Qualifiers
     source          1..10768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4113..4122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6625..7213
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6678..6698)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7096..7107)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7285..7518
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7596..7717
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7633..7652)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7687..7706
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7757..7892
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7912..7931)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7913..7931)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7941..7958)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7941..7957)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7950..7972)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8099..8554
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8186..8205)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8396..8417
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8580..8684
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8685..8753,8754..9545)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8903..8922)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9716..10304
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10205..10224
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10458..10475
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10592..10613
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10628..10633,10634..10651,10652..10658)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10666..10682
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10671..10693
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10690..10706
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10690..10706
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10725..10745
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10727..10745
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg gcgctggcgg aggagcagac
     4141 ggaggtggcg gtcaagctag agcctgaggg accgccaacg ctgctacctc cgcaggcggg
     4201 ggacggcgca ggcgagggta gcggcggcac taccaacaac ggccccaacg gcggcggcgg
     4261 gaacgttgcg gcgtcgtcgt ccactggcgg ggatggcggg acccccaagc ccacggtggc
     4321 tgtctccgcc gctgccccgg cgggggcggc cccggtgccc gccgctgctc cggacgccgg
     4381 cgctccgcat gaccgacaga ctctactggc cgtgctgcag ttcctacggc agagcaaact
     4441 ccgcgaggcc gaagaggcgc tgcgccgtga ggccgggctg ctggaggagg cagtggcggg
     4501 ctccggagcc ccgggagagg tggacagcgc cggcgctgag gtgaccagcg cgcttctcag
     4561 ccgggtgacc gcctcggccc ctggccctgc ggcccccgac cctccgggca ctggcgcttc
     4621 gggggccacg gtcgtctcag gttcagcctc aggtcctgcg gctccgggta aagttggaag
     4681 tgttgctgtg gaagaccagc cagatgtcag tgccgtgttg tcagcctaca accaacaagg
     4741 agatcccaca atgtatgaag aatactatag tggactgaaa cacttcattg aatgttccct
     4801 ggactgccat cgggcagagt tgtcccaact tttttatcct ctgtttgtgc acatgtactt
     4861 ggagctagtc tacaatcaac atgagaatga agcaaagtca ttctttgaga agttccatgg
     4921 agatcaggaa tgttattacc aggatgacct acgagtatta tctagtctta ccaaaaagga
     4981 acacatgaaa gggaatgaga ccatgttgga ttttcgaaca agtaaatttg ttctgcgtat
     5041 ttcccgtgac tcgtaccaac tcttgaagag gcatcttcag gagaaacaga acaatcagat
     5101 atggaacata gttcaggagc acctctacat tgacatcttt gatgggatgc cgcgtagtaa
     5161 gcaacagata gatgcgatgg tgggaagttt ggcaggagag gctaaacgag aggcaaacaa
     5221 atcaaaggta ttttttggtt tattaaaaga accagaaatt gaggtacctt tggatgacga
     5281 ggatgaagag ggagaaaatg aagaaggaaa acctaaaaag aagaagccta aaaaagatag
     5341 tattggatcc aaaagcaaaa aacaagatcc caatgctcca cctcagaaca gaatccctct
     5401 tcctgagttg aaagattcag ataagttgga taagataatg aatatgaaag aaaccaccaa
     5461 acgagtgcgc cttgggccgg actgcttacc ctccatttgt ttctatacat ttctcaatgc
     5521 ttaccagggt ctcactgcag tggatgtcac tgatgattct agtctgattg ctggaggttt
     5581 tgcagattca actgtcagag tgtggtcggt aacacccaaa aagcttcgta gtgtcaaaca
     5641 agcatcagat cttagtctta tagacaaaga atcagatgat gtcttagaaa gaatcatgga
     5701 tgagaaaaca gcaagtgagt tgaagatttt gtatggtcac agtgggcctg tctacggagc
     5761 cagcttcagt ccggatagga actatctgct ttcctcttca gaggacggaa ctgttagatt
     5821 gtggagcctt caaacattta cttgtttggt gggatataaa ggacacaact atccagtatg
     5881 ggacacacaa ttttctccat atggatatta ttttgtgtca gggggccatg accgagtagc
     5941 tcggctctgg gctacagacc actatcagcc tttaagaata tttgccggcc atcttgctga
     6001 tgtgaattgt accagattcc atccaaattc taattatgtt gctacgggct ctgcagacag
     6061 aactgtgcgg ctctgggacg tcctgaatgg taactgtgta aggatcttca ctggacacaa
     6121 gggaccaatt cattccttga cattttctcc caatgggaga ttcctggcta caggagcaac
     6181 agatggcaga gtgcttcttt gggatattgg acatggtttg atggttggag aattaaaagg
     6241 ccacactgat acagtctgtt cacttaggtt tagtagagat ggtgaaattt tggcatcagg
     6301 ttcaatggat aatacagttc gattatggga tgctatcaaa gcctttgaag atttagagac
     6361 cgatgacttt actacagcca ctgggcatat aaatttacct gagaattcac aggagttatt
     6421 gttgggaaca tatatgacca aatcaacacc agttgtacac cttcatttta ctcgaagaaa
     6481 cctggttcta gctgcaggag cttatagtcc acaatagtaa tgaactagtc cgtaacttga
     6541 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaagatg atcaccctgg
     6601 attagcgtta cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     6661 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6721 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6781 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6841 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6901 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6961 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     7021 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7081 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7141 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7201 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7261 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     7321 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7381 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7441 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7501 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     7561 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     7621 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7681 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     7741 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7801 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     7861 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     7921 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     7981 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     8041 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     8101 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     8161 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     8221 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     8281 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     8341 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     8401 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     8461 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     8521 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     8581 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     8641 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     8701 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     8761 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     8821 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     8881 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     8941 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     9001 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     9061 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     9121 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     9181 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     9241 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     9301 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     9361 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     9421 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     9481 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     9541 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     9601 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9661 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     9721 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     9781 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     9841 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     9901 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9961 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    10021 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    10081 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    10141 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    10201 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    10261 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    10321 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    10381 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    10441 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    10501 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    10561 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    10621 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    10681 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
    10741 aaagggaaca aaagctggag ctgcaagc
//
