LOCUS       sequence-397866-       10051 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1597
FEATURES             Location/Qualifiers
     source          1..10051
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5908..6496
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5961..5981)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6379..6390)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6568..6801
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6879..7000
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6916..6935)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6970..6989
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7040..7175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7195..7214)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7196..7214)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7224..7241)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7224..7240)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7233..7255)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7382..7837
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7469..7488)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7679..7700
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7863..7967
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7968..8036,8037..8828)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8186..8205)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8999..9587
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9488..9507
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9741..9758
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9875..9896
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9911..9916,9917..9934,9935..9941)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9949..9965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9954..9976
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9973..9989
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9973..9989
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10008..10028
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10010..10028
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggct ggtgctcaga ctctgttgac
     4141 gttcagggat gtggccatag aattctccct ggaggagtgg aaatgcctgg acctcgctca
     4201 gcagaatttg tacagggatg tgatgttgga gaactacaga aacttgttct ccgttggtct
     4261 cactgtctgt aagccaggcc tgatcacctg cctggagcaa cgaaaagagc cctggaatgt
     4321 gaagagacag gaggcagcag acggacatcc agctatgtct tctcatttta cccaagacct
     4381 tctgccagag cagggcatac aagatgcatt cccaaaaaga atactgagag gatatggaaa
     4441 ttgtggcctt gataatttat atttaaggaa agactgggaa agtttagatg agtgtaagtt
     4501 gcaaaaagat tataatggac ttaaccaatg ttcatcaact acccatagca aaatctttca
     4561 atataataaa tatgttaaaa tctttgataa cttttcaaat ttacatagac gtaatataag
     4621 taatactgga gagaaacctt tcaaatgtca agaatgtggc aaatcctttc aaatgctctc
     4681 attcctaact gaacatcaga aaattcacac tggaaaaaaa ttccaaaaat gtggagaatg
     4741 tggcaaaacc tttatccagt gctcacactt tactgaacct gagaacattg acactggaga
     4801 gaaaccttac aagtgtcaag aatgtaacaa cgtcattaaa acttgctcag tccttactaa
     4861 aaatagaatt tatgccggag gggaacatta cagatgtgaa gaatttggca aagtatttaa
     4921 ccagtgctcc caccttactg aacatgagca tggtactgag gaaaaaccct gcaaatatga
     4981 agagtgcagc agtgtcttta tctcttgctc aagcctttct aatcaacaga tgattcttgc
     5041 tggagagaag ctctccaaat gtgaaacatg gtacaaaggt tttaaccaca gcccaaatcc
     5101 ttccaaacac cagagaaatg agattggagg gaaacctttc aaatgtgagg aatgtgacag
     5161 catcttcaag tggttctcag accttactaa acataagaga attcacactg gtgagaaacc
     5221 atacaaatgt gacgaatgtg ggaaagccta tacacagtcc tcacacctca gtgaacacag
     5281 gaggattcac accggagaga aaccctacca atgtgaagaa tgtgggaagg tcttcagaac
     5341 ttgctcaagc ctttctaacc ataagagaac tcattctgaa gaaaaaccct acacgtgtga
     5401 agaatgtggc aacatcttta agcagttatc agacctcact aagcataaga aaacccatac
     5461 tggagagaag ccctacaaat gtgacgaatg tggaaaaaac tttacccagt cctccaacct
     5521 tattgtacat aagagaattc atactggaga gaaaccctac aagtgtgaag aatgtggcag
     5581 agtcttcatg tggttctcag acattaccaa acataagaaa acccatactg gagagaaacc
     5641 ctacaaatgt gacgaatgtg gaaaaaactt tacccagtcc tcaaacctta ttgtacataa
     5701 gagaattcat actggagaga aaccctacaa gtgtgaaaag tgtggcaaag ccttcaccca
     5761 gttctcacac ctgactgtac atgaaagcat tcatacttag taatgaacta gtccgtaact
     5821 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaag ctcccgctcg
     5881 actcagactg taacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     5941 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6001 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6061 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6121 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6181 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6241 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6301 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     6361 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     6421 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     6481 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     6541 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     6601 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6661 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6721 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6781 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     6841 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     6901 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6961 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     7021 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7081 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7141 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     7201 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     7261 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     7321 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     7381 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7441 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7501 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7561 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7621 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7681 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7741 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7801 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     7861 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7921 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7981 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8041 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8101 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8161 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8221 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8281 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8341 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8401 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8461 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8521 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8581 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8641 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8701 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8761 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8821 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8881 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8941 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9001 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9061 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9121 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9181 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9241 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9301 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9361 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9421 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9481 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9541 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9601 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9661 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9721 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     9781 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     9841 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     9901 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     9961 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    10021 actaaaggga acaaaagctg gagctgcaag c
//
