LOCUS       sequence-397872-       11551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11551)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0686
FEATURES             Location/Qualifiers
     source          1..11551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7240..7257
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    7408..7996
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7461..7481)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7879..7890)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8068..8301
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8379..8500
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8416..8435)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8470..8489
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8540..8675
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8695..8714)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8696..8714)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8724..8741)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8724..8740)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8733..8755)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8882..9337
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8969..8988)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9179..9200
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9363..9467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9468..9536,9537..10328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9686..9705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10499..11087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10988..11007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11241..11258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11375..11396
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11411..11416,11417..11434,11435..11441)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11449..11465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11454..11476
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11473..11489
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11473..11489
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11508..11528
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11510..11528
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtct gcttactctg tgccttcaac
     4141 ttttgcccag gcctcattgc cagttcataa ccaggtgctg ccttccatcg agagtgtaga
     4201 tgggtccgac cctttggcaa ctctgcagac ccctctaggc agactggagg ccaaagagga
     4261 agaggatgag gatgaggacg aggacactga ggaagatgag gaagaagacg gtgaggacac
     4321 ggatctggat gactgggagc cagacccgcc ccggcccttc gacccacacg acttgtggtg
     4381 tgaggagtgc aataacgcgc atgcttcagt gtgtccgaag cacggcccct tgcacccgat
     4441 ccccaaccgg ccggtgctca cccgggccag ggcgagcctc cccctggtgc tctacataga
     4501 caggtttctg ggcggggtgt tctccaagcg gcgcatcccc aagcgcaccc agtttggccc
     4561 cgtggagggg cctctcgtca ggggctcgga gctgaaagac tgttacattc acctcaaggt
     4621 ttctcttgat aaaggggaca ggaaagaaag ggatttacat gaagacctat ggtttgagtt
     4681 gtctgatgag acgctttgta actggatgat gtttgtacgg ccagcccaga atcacctgga
     4741 gcagaacctg gtggcttacc agtatggcca ccatgtgtat tatacaacca taaaaaatgt
     4801 ggagcccaag caggaactga aggtgtggta tgccgcatcc tatgctgagt tcgtgaacca
     4861 gaaaattcat gacatttctg aggaagaaag gaaagttctt cgagagcaag agaagaattg
     4921 gccctgctat gaatgtaacc gccgatttat aagctcggag cagttgcaac agcatctcaa
     4981 ttctcatgat gagaaactag atgtgtttag cagaacaaga ggcagaggaa ggggacgagg
     5041 caagaggcga ttcggtccag gtcgacggcc ggggcgtcct ccaaaattta tccgcctgga
     5101 aatcaccagc gaaaatgggg aaaagagtga cgatgggaca caggacttgc tacattttcc
     5161 cacaaaggag caatttgatg aggctgaacc agccactctg aatgggctgg atcaaccaga
     5221 acagaccact atcccaatcc ctcagctgcc acaggaaacc cagtcttccc tggaacatga
     5281 accagaaact cacaccctgc acctgcagcc gcagcatgaa gagagcgtgg tgcccaccca
     5341 gagcacgctg acagccgacg acatgcgcag agccaagcgc atccgattgg agctgcagaa
     5401 tgcagctctt cagcatctgt ttattcggaa gtccttccgg ccttttaaat gcttgcagtg
     5461 tgggaaggcc ttccgggaaa aggacaaact ggaccagcac ttacgcttcc atgggcggga
     5521 ggggaactgc ccactgacct gtgatctctg taacaagggc ttcatcagca gcacatcctt
     5581 ggagagccac atgaagctcc actcagacca gaagacttac tcttgcattt tttgcccaga
     5641 atcctttgac cgccttgatt tgttgaaaga tcatgtggcc attcatatca atgatggcta
     5701 cttcacctgc ccaacttgta agaaacggtt cccagatttt atccaggtga aaaaacacgt
     5761 gcgcagcttc cactcagaaa agatctacca gtgcacagag tgtgacaagg ccttctgtcg
     5821 ccccgataaa ctgcgactcc acatgctccg gcattcggac cgcaaagact tcctgtgttc
     5881 cacctgtggg aagcaattta agcgaaaaga caaactacgg gaacacatgc agaggatgca
     5941 taatcctgag agggaggcca agaaagccga ccgcatcagc cgctccaaga cgttcaagcc
     6001 ccgcatcacg tccacagact acgacagctt cacgttcaag tgccgcctgt gcatgatggg
     6061 cttccggcgg cgcggcatgc tggtaaatca cttatcgaag agacacccag acatgaagat
     6121 agaagaggtg ccagagttaa ctctacccat cataaaaccc aatcgtgatt acttttgtca
     6181 gtattgcgat aaggtttata aaagtgccag caagcgcaaa gcccacattc tgaagaacca
     6241 cccaggagca gagctcccac cgagcattcg gaagctccga cccgctggtc ctggagagcc
     6301 agaccccatg ctgagcacac acacccagct gacgggcacc atcgccaccc ctcccgtctg
     6361 ctgtccccac tgctccaagc agtacagcag caagaccaag atggtccagc acattcgaaa
     6421 gaagcatcca gagttcgccc agctctccaa caccatacac acaccactga cgacagctgt
     6481 gatcagtgcc accccagcgg ttttgactac agacagcgcc actggagaga ctgtggtgac
     6541 gacggacctg ctcacccaag caatgacaga actgtcccag accttaacga cagactaccg
     6601 aacgccacaa ggggattacc agagaattca gtacatccct gtgtcgcagt cggcgtctgg
     6661 cctccagcag cctcagcaca tacagctgca agtggttcaa gtggcctcgg ccacttcccc
     6721 tcaccagtca cagcagtcca ctgtggatgt tggccagctc catgatcctc agccctaccc
     6781 ccagcacgcc atccaggtgc agcacatcca ggtatctggg cagccgttga gtccctcagc
     6841 ccagcaggct cagcaggggc tcagcccctc ccacatccag ggcagttctt ccacacaggg
     6901 gcaggctctg cagcagcagc agcagcagca gcagaattcc tctgtgcagc acacgtacct
     6961 gcccagtgct tggaattcct tccgtggcta ttcatctgag attcaaatga tgacgcttcc
     7021 tccgggtcag tttgtgatta cagacagtgg tgtggcaact ccagttacta ctggccaggt
     7081 gaaggcggtt acttcgggtc attatgtgtt atcagaaagt caatcagaat tggaagaaaa
     7141 gcaaacttct gccctctctg gtggagtcca ggtcgagcca cctgcacaca gtgactccct
     7201 ggacccccag accaacagcc aacagcagac cacacagtac atcatcacca ccaccaccaa
     7261 cgggaacgga agcagcgaag tgcatatcac caaaccatag taatgaacta gtccgtaact
     7321 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgagc acttaatcaa
     7381 tccacgtcaa gaacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     7441 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7501 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7561 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7621 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7681 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7741 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7801 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7861 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7921 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7981 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     8041 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     8101 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8161 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8221 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8281 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     8341 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     8401 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     8461 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     8521 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8581 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     8641 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     8701 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     8761 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     8821 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     8881 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8941 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     9001 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     9061 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     9121 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     9181 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     9241 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     9301 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     9361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     9421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     9481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     9541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     9601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     9661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     9721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     9781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     9841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     9901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     9961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    10021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    10081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    10141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    10201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    10261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    10321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    10381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    10441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    10501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    10561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    10621 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    10681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    10741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    10801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10861 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    10921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    11041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    11101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    11161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    11221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    11281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    11341 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    11401 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    11461 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    11521 actaaaggga acaaaagctg gagctgcaag c
//
