LOCUS       sequence-397890-       10171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0216
FEATURES             Location/Qualifiers
     source          1..10171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     RBS             4685..4693
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    6028..6616
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6081..6101)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6499..6510)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6688..6921
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6999..7120
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7036..7055)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7090..7109
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7160..7295
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7315..7334)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7316..7334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7344..7361)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7344..7360)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7353..7375)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7502..7957
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7589..7608)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7799..7820
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7983..8087
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8088..8156,8157..8948)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8306..8325)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9119..9707
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9608..9627
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9861..9878
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9995..10016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10031..10036,10037..10054,10055..10061)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10069..10085
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10074..10096
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10093..10109
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10093..10109
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10128..10148
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10130..10148
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgatg caagaatctg ggactgagac
     4141 aaaaagtaac ggttcagcca tccagaatgg gtcgggcggc agcaaccact tactagagtg
     4201 cggcggtctt cgggaggggc ggtccaacgg agagacgccg gccgtggaca tcggggcagc
     4261 tgacctcgcc cacgcccagc agcagcagca acaggcactt caggtggcaa gacagctcct
     4321 tcttcagcag caacagcagc agcaagttag tggattaaaa tctcccaaga ggaatgacaa
     4381 acaaccagct cttcagcaac agcaggtggc tacccagcag ttggcttttc agcagcagct
     4441 tttacagatg cagcagttac agcagcagca cctcctgtct ttgcagcgcc aaggccttct
     4501 gacaattcag cccgggcagc ctgcccttcc ccttcaacct cttgctcaag gcatgattcc
     4561 aacagaactg cagcagctct ggaaagaagt gacaagtgct catactgcag aagaaaccac
     4621 aggcaacaat cacagcagtt tggatctgac cacgacatgt gtctcctcct ctgcaccttc
     4681 caagacctcc ttaataatga acccacatgc ctctaccaat ggacagctct cagtccacac
     4741 tcccaaaagg gaaagtttgt cccatgagga gcacccccat agccatcctc tctatggaca
     4801 tggtgtatgc aagtggccag gctgtgaagc agtgtgcgaa gatttccaat catttctaaa
     4861 acatctcaac agtgagcatg cgctggacga tagaagtaca gcccaatgta gagtacaaat
     4921 gcaggttgta cagcagttag agctacagct tgcaaaagac aaagaacgcc tgcaagccat
     4981 gatgacccac ctgcatgtga agtctacaga acccaaagcc gcccctcagc ccttgaatct
     5041 ggtatcaagt gtcactctct ccaagtccgc atcggaggct tctccacaga gcttacctca
     5101 tactccaacg accccaaccg cccccctgac tcccgtcacc caaggcccct ctgtcatcac
     5161 aaccaccagc atgcacacgg tgggacccat ccgcaggcgg tactcagaca aatacaacgt
     5221 gcccatttcg tcagcagata ttgcgcagaa ccaagaattt tataagaacg cagaagttag
     5281 accaccattt acatatgcat ctttaattag gcaggccatt ctcgaatctc cagaaaagca
     5341 gctaacacta aatgagatct ataactggtt cacacgaatg tttgcttact tccgacgcaa
     5401 cgcggccacg tggaagaatg cagtgcgtca taatcttagt cttcacaagt gttttgtgcg
     5461 agtagaaaac gttaaagggg cagtatggac agtggatgaa gtagaattcc aaaaacgaag
     5521 gccacaaaag atcagtggta acccttccct tattaaaaac atgcagagca gccacgccta
     5581 ctgcacacct ctcaatgcag ctttacaggc ttcaatggct gagaatagta tacctctata
     5641 cactaccgct tccatgggaa atcccactct gggcaactta gccagcgcaa tacgggaaga
     5701 gctgaacggg gcaatggagc ataccaacag caacgagagt gacagcagtc caggcagatc
     5761 tcctatgcaa gccgtgcatc ctgtacacgt caaagaagag cccctcgatc cagaggaagc
     5821 tgaagggccc ctgtccttag tgacaacagc caaccacagt ccagattttg accatgacag
     5881 agattacgaa gatgaaccag taaacgagga catggagtag taatgaacta gtccgtaact
     5941 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaag cttgttggct
     6001 gcttaatgca gtacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     6061 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6121 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6181 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6241 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6301 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6361 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6421 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     6481 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     6541 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     6601 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     6661 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     6721 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6781 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6841 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6901 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     6961 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     7021 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7081 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     7141 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7201 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7261 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     7321 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     7381 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     7441 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     7501 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7561 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7621 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7681 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7741 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7801 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7861 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7921 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     7981 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8041 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8101 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8161 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8221 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8281 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8341 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8401 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8461 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8521 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8581 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8641 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8701 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8761 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8821 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8881 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8941 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9001 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9061 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9121 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9181 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9241 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9301 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9361 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9421 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9481 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9541 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9601 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9661 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9721 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9781 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9841 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     9901 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     9961 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    10021 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    10081 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    10141 actaaaggga acaaaagctg gagctgcaag c
//
