LOCUS       sequence-397898-       15586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15586)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0753
FEATURES             Location/Qualifiers
     source          1..15586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    11443..12031
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11496..11516)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11914..11925)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12103..12336
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12414..12535
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12451..12470)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12505..12524
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12575..12710
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12730..12749)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12731..12749)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12759..12776)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12759..12775)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12768..12790)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12917..13372
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13004..13023)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13214..13235
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13398..13502
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13503..13571,13572..14363)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13721..13740)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14534..15122
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15023..15042
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15276..15293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15410..15431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15446..15451,15452..15469,15470..15476)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15484..15500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15489..15511
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15508..15524
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15508..15524
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     15543..15563
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15545..15563
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggat cctgaacaaa gtgtcaaggg
     4141 caccaagaag gctgagggaa gtccccggaa gcggctgacc aaaggagagg ccattcagac
     4201 cagtgtttct tccagcgtcc catacccagg cagcggcaca gctgccaccc aagagagccc
     4261 cgcccaagag ctcttagccc cgcagccctt cccgggcccc tcatcagttc ttagggaagg
     4321 ctctcaggag aaaacgggcc agcagcagaa gccccccaaa aggcccccca tcgaagcatc
     4381 cgtccacatc tcacagcttc cgcagcaccc tctgacacca gcattcatgt cgcctggcaa
     4441 acctgagcat ctcctggagg ggtccacatg gcaactggtt gaccccatga gacctggacc
     4501 ctctggctcc ttcgtggccc ctgggctcca tcctcagagc cagctccttc cttcccacgc
     4561 ttccatcatt ccccccgagg accttcctgg agtccccaaa gtcttcgtgc ctcgtccttc
     4621 ccaggtctcc ttgaagccca cagaagaggc acacaagaag gagaggaagc cccagaagcc
     4681 aggcaagtac atctgccagt actgcagccg gccctgtgcc aagcccagcg tgctccagaa
     4741 gcacattcgc tcacacacag gtgagaggcc ctacccctgc ggcccctgtg gcttctcctt
     4801 caagaccaag agtaatctct acaagcacag gaagtcccat gcccaccgca tcaaagcagg
     4861 cctggcctca ggcatgggtg gcgagatgta cccacatggg ctggagatgg agcggatccc
     4921 tggggaagag tttgaggagc ccactgaggg agaaagcaca gattctgaag aggagactag
     4981 tgccacctct ggtcaccctg cagagctctc cccaagaccc aagcagcccc ttctctccag
     5041 cgggctatac agctctggga gccacagttc cagccacgaa cgctgttccc tgtcccagtc
     5101 cagcacagcc cagtcactcg aagacccccc tccatttgtg gaaccctcat ctgagcaccc
     5161 cctgagccat aaacctgaag acacccacac gattaagcag aagctggccc tccgcttaag
     5221 cgagaggaag aaggtgatcg atgagcaggc gtttctgagc ccaggcagca aagggagtac
     5281 tgagtctggg tatttctctc gctccgagag tgcagagcag caggtcagcc ccccaaacac
     5341 caacgccaag tcctacgctg agatcatctt tggcaagtgt gggcgaatag gacagcggac
     5401 cgccatgctg acagccacct ccacccagcc cctcctgccc ctgtccaccg aagacaagcc
     5461 cagcctggtg cctttgtctg taccccggac gcaggtgatc gagcacatca cgaagctcat
     5521 caccatcaac gaggccgtgg tggacaccag cgagatcgac agcgtgaagc caaggcggag
     5581 ctcactgtcc aggcgcagca gcatggagtc cccaaaatcc agcctctacc gggagcccct
     5641 gtcatcccac agtgagaaaa ccaagcctga acaatcactg ctgagcctcc agcacccgcc
     5701 cagtaccgcc ccccctgtgc ctctcctgag aagccactca atgccttctg ccgcctgcac
     5761 tatcagcacc ccccaccacc ccttccgagg tagctactcc ttcgatgacc atatcaccga
     5821 ctccgaagcc ctgagccaca gcagtcacgt gtttacctcc cacccccgga tgctgaagcg
     5881 ccagccggca atcgaattac ctttgggagg ggaatacagt tctgaggagc ctggcccaag
     5941 cagcaaagac acagcctcca agccctcgga cgaagtggaa cccaaggaaa gcgagcttac
     6001 caaaaagacc aagaagggtt tgaaaacaaa aggggtgatc tacgaatgta acatatgtgg
     6061 tgctcggtac aagaaaaggg ataactacga agcccacaaa aaatactact gctcagagct
     6121 tcagatcgca aagcccatct ctgcaggcac ccacacatct ccagaagctg aaaagagtca
     6181 gattgagcat gagccgtggt cccaaatgat gcattacaaa ctgggaacca ccctggaact
     6241 cactccactg aggaagagga ggaaagagaa gagccttggg gacgaggaag agccacctgc
     6301 ctttgagtcc acaaaaagtc agtttggcag ccccgggcca tctgatgctg ctcggaacct
     6361 tcccctggag tccaccaagt caccagcaga accaagtaaa tcagtgccct ccttggaggg
     6421 acccacgggc ttccagccaa ggactcccaa gccagggtcc ggttcagaat cagggaagga
     6481 gaggagaaca acgtccaaag aaatttctgt catccagcac accagctcct ttgagaaatc
     6541 tgattctctc gagcagccga gtggcttgga aggggaagac aaacctctgg cccagttccc
     6601 atcaccccca cctgccccac acggacgctc tgctcactcc ctgcagccta agttggtccg
     6661 ccagcccaac attcaggttc ctgagatcct agtaactgag gagcctgacc ggccggacac
     6721 agagccagag ccgcccccta aggaacctga gaagactgag gagttccaat ggccccagcg
     6781 cagccagaca cttgcccagc tcccagctga gaagctgcca cccaaaaaga agaggttgcg
     6841 cctggcagag atggcccaat catcagggga gtccagcttc gagtcctctg tgcctctgtc
     6901 tcgcagcccg agccaggaaa gcaatgtctc tttgagtggg tccagccgct cagcctcgtt
     6961 tgagagggat gaccatggga aagccgaggc ccccagtccc tcatctgaca tgcgccccaa
     7021 acccctgggc acccacatgt tgactgtccc cagccaccac ccacatgccc gagagatgcg
     7081 gaggtcagcc tcagagcaga gccccaacgt ttcccattct gcccacatga ccgagacacg
     7141 cagcaaatcc tttgactatg gcagcttgtc cttgacaggc ccttctgctc cagccccagt
     7201 ggctccacca gcgcgggtgg ccccgccaga gagaagaaaa tgcttcttgg tgagacaggc
     7261 ctctctgagc aggcctccag aatctgagtt ggaggttgcc cccaagggaa gacaggagag
     7321 cgaagaacca cagccctcat ccagtaaacc ctctgccaaa agctcattgt cccagatttc
     7381 ctctgcggcc acctcacatg gtggaccccc gggaggcaag ggcccagggc aggacaggcc
     7441 cccattgggg cccactgtgc cctacacaga agcactgcaa gtgttccacc accccgttgc
     7501 ccagacaccc ctgcatgaga agccatacct gcccccacca gtctcccttt tctccttcca
     7561 gcatctcgtg cagcatgagc caggacagtc tccagaattc ttctccaccc aggccatgtc
     7621 cagcctcctg tcctcaccat actccatgcc cccacttcct ccctccttat ttcaagcccc
     7681 accgcttcct ctccagccta ctgttctgca cccaggccaa ctccatctcc cccagctcat
     7741 gcctcaccca gccaacatcc ccttcaggca gcccccttcc ttcctcccca tgccataccc
     7801 gacctcctca gcactgtctt ctgggttttt cctgcctctg caatcccagt ttgcacttca
     7861 gctccctggt gatgtggaaa gccatctgcc ccagatcaaa accagcctgg ccccactggc
     7921 aacaggaagt gctggcctct cccccagcac agagtacagc agtgacatcc ggctaccccc
     7981 tgtggctccc ccagccagct cctcagcacc tacatcagct cctccactgg ccctgcctgc
     8041 ctgtccagac accatggtgt ccctggttgt gcctgtccgt gttcagacca atatgccgtc
     8101 ctatgggagc gcaatgtaca ccaccctttc ccagatcttg gtcacccagt cccaaggcag
     8161 ctcagcaact gtggcacttc ccaagtttga ggaaccccca tcaaagggga cgactgtatg
     8221 tggtgcagat gtgcatgagg ttgggcccgg cccttctggg ttaagtgaag agcaaagcag
     8281 agctttccca actccatacc tgagagtgcc tgtgacatta cctgaaagaa aaggcacttc
     8341 cctgtcatca gagagtatct tgagcctgga ggggagttca tcaacagcag ggggaagcaa
     8401 acgtgtcctt tcaccagctg gcagccttga acttaccatg gaaacccagc agcaaaaaag
     8461 agtgaaggag gaggaggctt ccaaggcaga tgaaaaactt gagctggtaa aaccatgcag
     8521 tgtggtcctt accagcaccg aggatgggaa gaggccagag aaatcccact taggcaacca
     8581 gggccaaggc aggagggagc tagaaatgct gtccagcctg tcctcagatc catctgacac
     8641 aaaggaaatt cctcccctcc ctcaccctgc attgtcccat gggacagccc caggctcaga
     8701 agctttgaag gaatatcccc agccatctgg caaacctcac cgaagagggt tgaccccact
     8761 gagcgtgaag aaagaagatt ccaaggaaca acctgatctc ccctccttgg cacctccgag
     8821 ctctctgcct ctgtcagaaa cgtcctccag accagccaag tcacaagaag gtacggactc
     8881 aaagaaggta ctgcagttcc ccagcctcca cacaaccact aatgtcagtt ggtgctattt
     8941 aaactacatt aagccaaatc acatccagca tgcagatagg aggtcctctg tttacgctgg
     9001 ttggtgcata agtttgtaca accccaacct tccgggggtt tccactaaag ctgctttgtc
     9061 cctcctgagg tctaagcaga aagtgagcaa agagacatac accatggcca cagctccgca
     9121 tcctgaggca ggaaggcttg tgccatccag ctcccgcaag ccccgcatga cagaggttca
     9181 cctcccttca ctggtttccc cggaaggcca gaaagatcta gctagagtgg agaaggaaga
     9241 agagaggaga ggggagccgg aggaggatgc tcctgcctcc cagagagggg agccggcgag
     9301 gatcaaaatc ttcgaaggag ggtacaaatc aaacgaagag tatgtatatg tgcgaggccg
     9361 cggccgaggg aaatatgttt gtgaggagtg tggaattcgc tgcaagaagc ccagcatgct
     9421 gaagaaacac atccgcaccc acactgacgt ccggccctat gtgtgcaagc actgtcactt
     9481 tgcttttaaa accaaaggga atctgactaa gcacatgaag tcgaaggccc acagcaaaaa
     9541 gtgccaagag acaggggtgc tggaggagct ggaagccgaa gaaggaacca gtgacgacct
     9601 gttccaggac tcggaaggac gagagggttc agaggctgtg gaggagcacc agttttcgga
     9661 cctggaggac tcggactcag actcagacct ggacgaagac gaggatgagg atgaggagga
     9721 gagccaggat gagctgtcca gaccatcctc agaggcgccc ccgcctggcc caccacatgc
     9781 actgcgggca gactcctcac ccatcctggg ccctcagccc ccagatgccc ccgcctctgg
     9841 cacggaggct acacgaggca gctcggtctc ggaagctgag cgcctgacag ccagcagctg
     9901 ctccatgtcc agccagagca tgccgggcct cccctggctg ggaccggccc ctctgggctc
     9961 tgtggagaaa gacacaggct cagccttgag ctacaagcct gtgtccccaa gaagaccgtg
    10021 gtccccaagc aaagaagcag gcagccgtcc accactagcc cgcaaacact cgctaaccaa
    10081 aaacgactca tctccccagc gatgctcccc ggcccgagaa ccacaggcct cagccccaag
    10141 cccacctggc ctgcacgtgg acccaggaag gggcatgggc gctctccctt gtgggtctcc
    10201 aagacttcag ctgtctcctc tcaccctctg ccccctggga agagaactgg cccctcgagc
    10261 acatgtgctc tccaaactcg agggtaccac cgacccaggc ctccccagat actcgcccac
    10321 caggagatgg tctccaggtc aggccgagtc accaccacgg tcagcgccgc cagggaagtg
    10381 ggccttggct gggccgggca gcccctcagc gggggagcat ggcccaggct tggggctgga
    10441 cccacgggtt ctcttcccgc ccgcgcctct acctcacaag ctcctcagca gaagcccaga
    10501 gacctgcgcc tccccgtggc agaaggccga gtcccgaagt ccctcctgct cacccggccc
    10561 tgctcatcct ctctcctccc gacccttctc cgccctccat gacttccacg gccacatcct
    10621 ggcccggaca gaggagaaca tcttcagcca cctgcctctg cactcccagc acttgacccg
    10681 tgccccatgt cccttgattc ccatcggtgg gatccagatg gtgcaggccc ggccaggagc
    10741 ccaccccacc ctgctgccag ggcccaccgc agcctgggtc agtggcttct ccgggggtgg
    10801 cagcgacctg acaggggccc gggaggccca ggagcgaggc cgctggagtc ccactgagag
    10861 ctcgtcagcc tccgtgtcgc ctgtggctaa ggtctccaaa ttcacactct cctcagagct
    10921 ggagggcggg gactacccca aggagaggga gaggaccggc ggaggcccgg gcaggcctcc
    10981 tgactggaca ccccatggga ccggggcacc tgcagagccc acacccacgc acagcccctg
    11041 caccccaccc gacaccttgc cccggccgcc ccagggacgc cgggcagcgc agtcctggag
    11101 cccccgcttg gagtccccgc gtgcaccgac caaccccgag ccttctgcca ccccgccgct
    11161 ggaccgcagc agctctgtgg gctgcctggc agaggcctct gcccgcttcc cagcccggac
    11221 gaggaacctc tccggggaac ccaggaccag gcaggactcc cccaagccct caggaagtgg
    11281 ggagcccagg gcacatccac atcagcctga ggacagggtt ccccccaacg cttagtaatg
    11341 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
    11401 cgaccaggaa gagggttaac ccacagcacg cgttaagtcg acaatcaacc tctggattac
    11461 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
    11521 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
    11581 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
    11641 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
    11701 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
    11761 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
    11821 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
    11881 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
    11941 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
    12001 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
    12061 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
    12121 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
    12181 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
    12241 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
    12301 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
    12361 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
    12421 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    12481 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    12541 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
    12601 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    12661 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
    12721 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
    12781 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    12841 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    12901 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    12961 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    13021 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    13081 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    13141 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    13201 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    13261 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    13321 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
    13381 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    13441 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    13501 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    13561 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    13621 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    13681 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    13741 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    13801 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    13861 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    13921 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    13981 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    14041 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    14101 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    14161 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    14221 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    14281 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    14341 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    14401 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    14461 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    14521 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    14581 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    14641 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    14701 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    14761 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    14821 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    14881 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    14941 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    15001 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    15061 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    15121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    15181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    15241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    15301 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    15361 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    15421 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    15481 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    15541 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagc
//
