LOCUS       sequence-397904-       11824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11824)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0805
FEATURES             Location/Qualifiers
     source          1..11824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7681..8269
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7734..7754)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8152..8163)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8341..8574
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8652..8773
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8689..8708)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8743..8762
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8813..8948
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8968..8987)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8969..8987)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8997..9014)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8997..9013)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9006..9028)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9155..9610
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9242..9261)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9452..9473
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9636..9740
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9741..9809,9810..10601)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9959..9978)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10772..11360
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11261..11280
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11514..11531
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11648..11669
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11684..11689,11690..11707,11708..11714)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11722..11738
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11727..11749
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11746..11762
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11746..11762
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11781..11801
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11783..11801
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg gtgcggaaga aggacggcgg
     4141 ccccaacgtg aagtactacg aggccgcgga caccgtgacc cagttcgaca acgtgcggct
     4201 gtggctcggc aagaactaca agaagtatat acaagctgaa ccacccacca acaagtccct
     4261 gtctagcctg gttgtacagt tgctacaatt tcaggaagaa gtttttggca aacatgtcag
     4321 caatgcaccg ctcactaaac tgccgatcaa atgtttccta gatttcaaag cgggaggctc
     4381 cttgtgccac attcttgcag ctgcctacaa attcaagagt gaccagggat ggcggcgtta
     4441 cgatttccag aatccatcac gcatggaccg caatgtggaa atgtttatga ccattgagaa
     4501 gtccttggtg cagaataatt gcctgtctcg acctaacatt tttctgtgcc cagaaattga
     4561 gcccaaacta ctagggaaat taaaggacat tatcaagaga caccagggaa cagtcactga
     4621 ggataagaac aatgcctccc atgttgtgta tcctgtcccg gggaatctag aagaagagga
     4681 atgggtacga ccagtcatga agagggataa gcaggttctt ctgcactggg gctactatcc
     4741 tgacagttac gacacgtgga tcccagcgag tgaaattgag gcatctgtgg aagatgctcc
     4801 aactcctgag aaacctagga aggttcatgc aaagtggatc ctggacaccg acaccttcaa
     4861 tgaatggatg aatgaggaag actatgaagt aaatgatgac aaaaaccctg tctcccgccg
     4921 aaagaagatt tcagccaaga cactgacaga tgaggtgaac agcccagatt cagatcgacg
     4981 ggacaagaag gggggaaact ataagaagag gaagcgctcc ccctctcctt caccaacccc
     5041 agaagcaaag aagaaaaatg ctaagaaagg tccctcaaca ccttacacta agtcaaagcg
     5101 tggccacaga gaagaggagc aagaagacct gacaaaggac atggacgagc cctcaccagt
     5161 ccccaatgta gaagaggtga cacttcccaa aacagtcaac acaaagaaag actcagagtc
     5221 ggccccagtc aaaggcggca ccatgaccga cctggatgaa caggaagatg aaagcatgga
     5281 gacgacgggc aaggatgagg atgagaacag tacggggaac aagggagagc agaccaagaa
     5341 tccagacctg catgaggaca atgtgactga acagacccac cacatcatca ttcccagcta
     5401 cgctgcctgg tttgactaca atagtgttca tgccattgag cggagggctc tccccgagtt
     5461 cttcaacggc aagaacaagt ccaagactcc agagatctac ctggcctatc gaaactttat
     5521 gattgacact taccgactga acccccaaga gtatcttacc tctaccgcct gccgccgaaa
     5581 cctagcgggt gatgtctgtg ccatcatgag ggtccatgcc ttcctagaac agtggggtct
     5641 tattaactac caggtggatg ctgagagtcg accaacccca atggggcctc cgcctacctc
     5701 tcacttccat gtcttggctg acacaccatc agggctggtg cctctgcagc ccaagacacc
     5761 tcagggccgc caggttgatg ctgataccaa ggctgggcga aagggcaaag agctggatga
     5821 cctggtgcca gagacggcta agggcaagcc agagctgcag acctctgctt cccaacaaat
     5881 gctcaacttt cctgacaaag gcaaagagaa accaacagac atgcaaaact ttgggctgcg
     5941 cacagacatg tacacaaaaa agaatgttcc ctccaagagc aaggctgcag ccagtgccac
     6001 tcgtgagtgg acagaacagg aaaccctgct tctcctggag gcactggaaa tgtacaaaga
     6061 tgactggaac aaagtgtccg agcatgtggg aagccgcaca caggacgagt gcatcttgca
     6121 ttttcttcgt cttcccattg aagacccata cctggaggac tcagaggcct ccctaggccc
     6181 cctggcctac caacccatcc ccttcagtca gtcgggcaac cctgttatga gcactgttgc
     6241 cttcctggcc tctgtcgtcg atccccgagt cgcctctgct gctgcaaagt cagccctaga
     6301 ggagttctcc aaaatgaagg aagaggtacc cacggccttg gtggaggccc atgttcgaaa
     6361 agtggaagaa gcagccaaag taacaggcaa ggcggaccct gccttcggtc tggaaagcag
     6421 tggcattgca ggaaccacct ctgatgagcc tgagcggatt gaggagagcg ggaatgacga
     6481 ggctcgggtg gaaggccagg ccacagatga gaagaaggag cccaaggaac cccgagaagg
     6541 agggggtgct atagaggagg aagcaaaaga gaaaaccagc gaggctccca agaaggatga
     6601 ggagaaaggg aaagaaggcg acagtgagaa ggagtccgag aagagtgatg gagacccaat
     6661 agtcgatcct gagaaggaga aggagccaaa ggaagggcag gaggaagtgc tgaaggaagt
     6721 ggtggagtct gagggggaaa ggaagacaaa ggtggagcgg gacattggcg agggcaacct
     6781 ctccaccgct gctgccgccg ccctggccgc cgccgcagtg aaagctaagc acttggctgc
     6841 tgttgaggaa aggaagatca aatctttggt ggccctgctg gtggagaccc agatgaaaaa
     6901 gttggagatc aaacttcggc actttgagga gctggagact atcatggacc gggagcgaga
     6961 agcactggag tatcagaggc agcagctcct ggccgacaga caagccttcc acatggagca
     7021 gctgaagtat gcggagatga gggctcggca gcagcacttc caacagatgc accaacagca
     7081 gcagcagcca ccaccagccc tgcccccagg ctcccagcct atccccccaa caggggctgc
     7141 tgggccaccc gcagtccatg gcttggctgt ggctccagcc tctgtagtcc ctgctcctgc
     7201 tggcagtggg gcccctccag gaagtttggg cccttctgaa cagattgggc aggcagggtc
     7261 aactgcaggg ccacagcagc agcaaccagc tggagccccc cagcctgggg cagtcccacc
     7321 aggggttccc ccccctggac cccatggccc ctcaccgttc cccaaccaac aaactcctcc
     7381 ctcaatgatg ccaggggcag tgccaggcag cgggcaccca ggcgtggcgg cccaaagccc
     7441 tgccattgtg gcagctgttc agggcaacct cctgcccagt gccagcccac tgccagaccc
     7501 aggcaccccc ctgcctccag accccacagc cccgagccca ggcacggtca cccctgtgcc
     7561 acctccacag tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
     7621 tatatcttgt ggaaaggacg agtctacctg ctgccttggt tctctacgcg ttaagtcgac
     7681 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7741 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7801 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7861 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7921 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7981 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     8041 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     8101 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     8161 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     8221 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     8281 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8341 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     8401 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8461 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8521 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     8581 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     8641 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8701 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8761 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     8821 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     8881 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     8941 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     9001 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     9061 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     9121 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     9181 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     9241 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     9301 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     9361 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     9421 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     9481 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     9541 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     9601 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     9661 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     9721 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     9781 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     9841 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     9901 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     9961 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
    10021 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    10081 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    10141 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    10201 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    10261 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    10321 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    10381 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    10441 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    10501 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    10561 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    10621 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    10681 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    10741 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    10801 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    10861 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    10921 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    10981 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    11041 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    11101 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    11161 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    11221 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    11281 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    11341 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    11401 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    11461 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    11521 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    11581 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    11641 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    11701 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    11761 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
    11821 aagc
//
