LOCUS       sequence-397908-       12760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12760)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0915
FEATURES             Location/Qualifiers
     source          1..12760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8617..9205
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8670..8690)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9088..9099)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9277..9510
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9588..9709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9625..9644)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9679..9698
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9749..9884
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9904..9923)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9905..9923)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9933..9950)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9933..9949)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9942..9964)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10091..10546
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10178..10197)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10388..10409
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10572..10676
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10677..10745,10746..11537)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10895..10914)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11708..12296
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12197..12216
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12450..12467
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12584..12605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12620..12625,12626..12643,12644..12650)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12658..12674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12663..12685
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12682..12698
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12682..12698
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12717..12737
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12719..12737
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgagt gggatgggag aaaatacctc
     4141 tgacccctcc agggcagaga caagaaagcg caaggaatgt cctgaccaac ttggacccag
     4201 ccccaaaagg aacactgaaa aacgtaatcg tgaacaggaa aataaatata tagaagaact
     4261 tgcagagttg atttttgcaa attttaatga tatagacaac tttaacttca aacctgacaa
     4321 atgtgcaatc ttaaaagaaa ctgtgaagca aattcgtcag atcaaagaac aagagaaagc
     4381 agcagctgcc aacatagatg aagtgcagaa gtcagatgta tcctctacag ggcagggtgt
     4441 catcgacaag gatgcgctgg ggcctatgat gcttgaggcc cttgatgggt tcttctttgt
     4501 agtgaacctg gaaggcaacg ttgtgtttgt gtcagagaat gtgacacagt atctaaggta
     4561 taaccaagaa gagctgatga acaaaagtgt atatagcatc ttgcatgttg gggaccacac
     4621 ggaatttgtc aaaaacctgc tgccaaagtc tatagtaaat gggggatctt ggtctggcga
     4681 acctccgagg cggaacagcc ataccttcaa ttgtcggatg ctggtaaaac ctttacctga
     4741 ttcagaagag gagggtcatg ataaccagga agctcatcag aaatatgaaa ctatgcagtg
     4801 cttcgctgtc tctcaaccaa agtccatcaa agaagaagga gaagatttgc agtcctgctt
     4861 gatttgcgtg gcaagaagag ttcccatgaa ggaaagacca gttcttccct catcagaaag
     4921 ttttactact cgccaggatc tccaaggcaa gatcacgtct ctggatacca gcaccatgag
     4981 agcagccatg aaaccaggct gggaggacct ggtaagaagg tgtattcaga agttccatgc
     5041 gcagcatgaa ggagaatctg tgtcctatgc taagaggcat catcatgaag tactgagaca
     5101 aggattggca ttcagtcaaa tctatcgttt ttccttgtct gatggcactc ttgttgctgc
     5161 acaaacgaag agcaaactca tccgttctca gactactaat gaacctcaac ttgtaatatc
     5221 tttacatatg cttcacagag agcagaatgt gtgtgtgatg aatccggatc tgactggaca
     5281 aacgatgggg aagccactga atccaattag ctctaacagc cctgcccatc aggccctgtg
     5341 cagtgggaac ccaggtcagg acatgaccct cagtagcaat ataaattttc ccataaatgg
     5401 cccaaaggaa caaatgggca tgcccatggg caggtttggt ggttctgggg gaatgaacca
     5461 tgtgtcaggc atgcaagcaa ccactcctca gggtagtaac tatgcactca aaatgaacag
     5521 cccctcacaa agcagccctg gcatgaatcc aggacagccc acctccatgc tttcaccaag
     5581 gcatcgcatg agccctggag tggctggcag ccctcgaatc ccacccagtc agttttcccc
     5641 tgcaggaagc ttgcattccc ctgtgggagt ttgcagcagc acaggaaata gccatagtta
     5701 taccaacagc tccctcaatg cacttcaggc cctcagcgag gggcacgggg tctcattagg
     5761 gtcatcgttg gcttcaccag acctaaaaat gggcaatttg caaaactccc cagttaatat
     5821 gaatcctccc ccactcagca agatgggaag cttggactca aaagactgtt ttggactata
     5881 tggggagccc tctgaaggta caactggaca agcagagagc agctgccatc ctggagagca
     5941 aaaggaaaca aatgacccca acctgccccc ggccgtgagc agtgagagag ctgacgggca
     6001 gagcagactg catgacagca aagggcagac caaactcctg cagctgctga ccaccaaatc
     6061 tgatcagatg gagccctcgc ccttagccag ctctttgtcg gatacaaaca aagactccac
     6121 aggtagcttg cctggttctg ggtctacaca tggaacctcg ctcaaggaga agcataaaat
     6181 tttgcacaga ctcttgcagg acagcagttc ccctgtggac ttggccaagt taacagcaga
     6241 agccacaggc aaagacctga gccaggagtc cagcagcaca gctcctggat cagaagtgac
     6301 tattaaacaa gagccggtga gccccaagaa gaaagagaat gcactacttc gctatttgct
     6361 agataaagat gatactaaag atattggttt accagaaata acccccaaac ttgagagact
     6421 ggacagtaag acagatcctg ccagtaacac aaaattaata gcaatgaaaa ctgagaagga
     6481 ggagatgagc tttgagcctg gtgaccagcc tggcagtgag ctggacaact tggaggagat
     6541 tttggatgat ttgcagaata gtcaattacc acagcttttc ccagacacga ggccaggcgc
     6601 ccctgctgga tcagttgaca agcaagccat catcaatgac ctcatgcaac tcacagctga
     6661 aaacagccct gtcacacctg ttggagccca gaaaacagca ctgcgaattt cacagagcac
     6721 ttttaataac ccacgaccag ggcaactggg caggttattg ccaaaccaga atttaccact
     6781 tgacatcaca ttgcaaagcc caactggtgc tggacctttc ccaccaatca gaaacagtag
     6841 tccctactca gtgatacctc agccaggaat gatgggtaat caagggatga taggaaacca
     6901 aggaaattta gggaacagta gcacaggaat gattggtaac agtgcttctc ggcctactat
     6961 gccatctgga gaatgggcac cgcagagttc ggctgtgaga gtcacctgtg ctgctaccac
     7021 cagtgccatg aaccggccag tccaaggagg tatgattcgg aacccagcag ccagcatccc
     7081 catgaggccc agcagccagc ctggccaaag acagacgctt cagtctcagg tcatgaatat
     7141 agggccatct gaattagaga tgaacatggg gggacctcag tatagccaac aacaagctcc
     7201 tccaaatcag actgccccat ggcctgaaag catcctgcct atagaccagg cgtcttttgc
     7261 cagccaaaac aggcagccat ttggcagttc tccagatgac ttgctatgtc cacatcctgc
     7321 agctgagtct ccgagtgatg agggagctct cctggaccag ctgtatctgg ccttgcggaa
     7381 ttttgatggc ctggaggaga ttgatagagc cttaggaata cccgaactgg tcagccagag
     7441 ccaagcagta gatccagaac agttctcaag tcaggattcc aacatcatgc tggagcagaa
     7501 ggcgcccgtt ttcccacagc agtatgcatc tcaggcacaa atggcccagg gtagctattc
     7561 tcccatgcaa gatccaaact ttcacaccat gggacagcgg cctagttatg ccacactccg
     7621 tatgcagccc agaccgggcc tcaggcccac gggcctagtg cagaaccagc caaatcaact
     7681 aagacttcaa cttcagcatc gcctccaagc acagcagaat cgccagccac ttatgaatca
     7741 aatcagcaat gtttccaatg tgaacttgac tctgaggcct ggagtaccaa cacaggcacc
     7801 tattaatgca cagatgctgg cccagagaca gagggaaatc ctgaaccagc atcttcgaca
     7861 gagacaaatg catcagcaac agcaagttca gcaacgaact ttgatgatga gaggacaagg
     7921 gttgaatatg acaccaagca tggtggctcc tagtggtatg ccagcaacta tgagcaaccc
     7981 tcggattccc caggcaaatg cacagcagtt tccatttcct ccaaactacg gaataagtca
     8041 gcaacctgat ccaggcttta ctggggctac gactccccag agcccactta tgtcaccccg
     8101 aatggcacat acacagagtc ccatgatgca acagtctcag gccaacccag cctatcaggc
     8161 cccctccgac ataaatggat gggcgcaggg gaacatgggc ggaaacagca tgttttccca
     8221 gcagtcccca ccacactttg ggcagcaagc aaacaccagc atgtacagta acaacatgaa
     8281 catcaatgtg tccatggcga ccaacacagg tggcatgagc agcatgaacc agatgacagg
     8341 acagatcagc atgacctcag tgacctccgt gcctacgtca gggctgtcct ccatgggtcc
     8401 cgagcaggtt aatgatcctg ctctgagggg aggcaacctg ttcccaaacc agctgcctgg
     8461 aatggatatg attaagcagg agggagacac aacacggaaa tattgctagt aatgaactag
     8521 tccgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacgg
     8581 gagcaggcat cggtaatgag gacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     8641 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     8701 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     8761 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     8821 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     8881 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     8941 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     9001 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     9061 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     9121 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     9181 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     9241 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     9301 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     9361 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     9421 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     9481 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     9541 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     9601 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9661 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     9721 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     9781 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     9841 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     9901 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     9961 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
    10021 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
    10081 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
    10141 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
    10201 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
    10261 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
    10321 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
    10381 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
    10441 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
    10501 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
    10561 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
    10621 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
    10681 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
    10741 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
    10801 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
    10861 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
    10921 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
    10981 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
    11041 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
    11101 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
    11161 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
    11221 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
    11281 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
    11341 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    11401 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    11461 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    11521 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    11581 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    11641 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    11701 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    11761 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    11821 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    11881 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    11941 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    12001 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    12061 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    12121 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    12181 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    12241 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    12301 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    12361 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    12421 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    12481 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    12541 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    12601 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    12661 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
    12721 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
//
