LOCUS       sequence-397919-       11110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0706
FEATURES             Location/Qualifiers
     source          1..11110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6967..7555
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7020..7040)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7438..7449)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7627..7860
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7938..8059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7975..7994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8029..8048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8099..8234
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8254..8273)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8255..8273)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8283..8300)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8283..8299)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8292..8314)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8441..8896
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8528..8547)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8738..8759
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8922..9026
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9027..9095,9096..9887)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9245..9264)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10058..10646
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10547..10566
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10800..10817
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10934..10955
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10970..10975,10976..10993,10994..11000)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11008..11024
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11013..11035
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11032..11048
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11032..11048
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11067..11087
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11069..11087
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtca agttcaggtt atcctcccaa
     4141 ccaaggagca ttcagcacag aacaaagtcg ttatcctcct cactctgtcc agtatacatt
     4201 tcccaacacc cgccaccagc aggatccagc attcggaggc aaacatgaag ctccatcctc
     4261 tccaatttcg gggcaaccat gtggagatga tcaaaatgct tcaccttcaa aactctcaaa
     4321 ggaagagtta atacagagta tggatcgtgt agatcgagaa attgcaaaag tagaacagca
     4381 gatccttaaa ctgaaaaaga aacaacaaca gcttgaagaa gaggcagcta aacctcctga
     4441 gcctgagaag cccgtgtccc ctcctcctgt ggagcagaaa caccgcagta ttgtccaaat
     4501 tatttatgat gagaatcgga aaaaagcaga agaagctcat aaaatttttg aaggtcttgg
     4561 cccaaaagtt gaactgccac tgtataacca gccatcagat accaaggtgt accatgagaa
     4621 catcaagaca aaccaggtga tgaggaaaaa actcatttta ttttttaaaa gaagaaatca
     4681 tgcaagaaaa caaagggaac aaaaaatctg ccagcgttat gatcagctca tggaggcatg
     4741 ggagaaaaaa gtggacagaa tagaaaataa tcctcggagg aaagctaaag aaagcaaaac
     4801 aagggaatac tatgaaaagc agtttccaga aattcgaaaa caaagagaac agcaagaaag
     4861 atttcagcga gttgggcaga ggggagctgg tctttcagcc accattgcta ggagtgagca
     4921 tgagatttct gaaattattg atgggctctc tgagcaggag aataatgaga aacaaatgcg
     4981 gcagctctct gtgattccac ctatgatgtt tgatgcagaa caaagacgag tcaagttcat
     5041 taacatgaat gggcttatgg aggaccctat gaaagtgtat aaagataggc agtttatgaa
     5101 tgtttggact gaccatgaaa aggagatctt taaggacaag tttatccagc atccaaaaaa
     5161 ctttggacta attgcatcat acttggagag gaagagtgtt cctgattgtg ttttgtatta
     5221 ctatttaacc aagaaaaatg agaattataa agccctcgtc agaaggaatt atgggaaacg
     5281 cagaggcaga aaccagcaaa ttgctcgacc ctcgcaagaa gaaaaagtag aagaaaaaga
     5341 agaggataaa gcagaaaaaa cagaaaaaaa agaagaagaa aagaaagatg aagaggaaaa
     5401 agatgaaaaa gaagactcca aagaaaatac caaggaaaag gacaagatag atggtacagc
     5461 agaagaaact gaggaaagag agcaagccac accccggggg cgaaagactg ccaacagtca
     5521 gggccgccgt aagggccgga tcaccaggtc catgacaaac gaagctgcag ctgccagtgc
     5581 tgcagccgca gcggctactg aagagccccc accacctctg ccaccgccac cagaacccat
     5641 ttctacagag cctgtggaga cctctcgatg gacagaagaa gaaatggaag ttgctaaaaa
     5701 aggtctagta gaacatggtc gtaactgggc agcaattgct aaaatggtgg gaacgaaaag
     5761 tgaagctcaa tgtaaaaact tctattttaa ctataaaagg cgacacaatc ttgacaacct
     5821 cttacagcag cataaacaga aaacttcacg aaaacctcgt gaagagcgag atgtgtctca
     5881 atgtgaaagt gtcgcttcca ctgtttctgc tcaggaggat gaagatattg aagcctccaa
     5941 tgaagaagaa aatccagaag acagcgaagg tgcagaaaat agttctgata cagaaagtgc
     6001 tccttctcct tcaccagttg aagctgtcaa gcccagcgag gacagtcctg aaaatgctac
     6061 ttctcgagga aacacagaac ctgcggttga gcttgagccc accacggaaa ctgcacccag
     6121 tacatctccc tccttagcag ttccaagtac aaaaccagct gaagatgaaa gtgtggagac
     6181 ccaggtgaat gacagcatca gtgctgagac agcagagcag atggatgtag atcagcagga
     6241 gcacagtgct gaagagggtt ctgtttgtga tcccccaccc gctaccaaag ctgactctgt
     6301 ggacgttgaa gtgagggtgc cagaaaacca tgcatctaaa gttgaaggtg ataataccaa
     6361 agaaagagac ttggatagag ccagtgagaa ggtggaacct agagatgaag atttggtggt
     6421 agctcagcaa ataaatgccc aaaggcccga gccccagtca gacaatgatt ccagtgccac
     6481 gtgcagcgct gatgaggatg tggatggaga gccagagagg cagagaatgt ttcctatgga
     6541 ctcaaagcct tcactgttaa accccactgg atctatactc gtctcatctc cgttaaaacc
     6601 aaatccactg gatctgccac agcttcagca tcgagctgct gttatcccac caatggtatc
     6661 ctgcacccca tgtaacatac caattggaac cccagtgagc ggctatgctc tctaccagcg
     6721 acacattaaa gcaatgcatg agtcagcact cctggaggag cagcggcaga gacaagaaca
     6781 gatagatttg gaatgtagaa gttctacaag tccatgtggc acatccaaga gtccaaacag
     6841 agagtgggaa ggtaggtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     6901 gctttatata tcttgtggaa aggacgacat atacagctgg gagttaccca aacgcgttaa
     6961 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     7021 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     7081 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7141 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7201 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7261 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7321 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     7381 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7441 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7501 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     7561 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7621 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     7681 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7741 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7801 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7861 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7921 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7981 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8041 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     8101 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8161 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8221 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     8281 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     8341 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     8401 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     8461 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     8521 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     8581 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     8641 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     8701 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     8761 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     8821 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     8881 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8941 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     9001 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     9061 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     9121 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     9181 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     9241 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     9301 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     9361 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     9421 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     9481 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     9541 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     9601 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     9661 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     9721 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9781 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9841 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9901 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9961 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    10021 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    10081 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    10141 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    10201 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    10261 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    10321 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    10381 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    10441 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    10501 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    10561 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    10621 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    10681 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    10741 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    10801 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    10861 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    10921 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    10981 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    11041 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    11101 agctgcaagc
//
