LOCUS       sequence-397923-       15580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15580)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1109
FEATURES             Location/Qualifiers
     source          1..15580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8867..8876
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    11437..12025
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11490..11510)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11908..11919)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12097..12330
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12408..12529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12445..12464)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12499..12518
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12569..12704
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12724..12743)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12725..12743)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12753..12770)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12753..12769)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12762..12784)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12911..13366
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12998..13017)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13208..13229
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13392..13496
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13497..13565,13566..14357)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13715..13734)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14528..15116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15017..15036
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15270..15287
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15404..15425
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15440..15445,15446..15463,15464..15470)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15478..15494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15483..15505
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15502..15518
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15502..15518
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     15537..15557
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15539..15557
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggct gagaacttgc tggacggacc
     4141 gcccaacccc aaaagagcca aactcagctc gcccggtttc tcggcgaatg acagcacaga
     4201 ttttggatca ttgtttgact tggaaaatga tcttcctgat gagctgatac ccaatggagg
     4261 agaattaggc cttttaaaca gtgggaacct tgttccagat gctgcttcca aacataaaca
     4321 actgtcggag cttctacgag gaggcagcgg ctctagtatc aacccaggaa taggaaatgt
     4381 gagcgccagc agccccgtgc agcagggcct gggtggccag gctcaagggc agccgaacag
     4441 tgctaacatg gccagcctca gtgccatggg caagagccct ctgagccagg gagattcttc
     4501 agcccccagc ctgcctaaac aggcagccag cacctctggg cccacccccg ctgcctccca
     4561 agcactgaat ccgcaagcac aaaagcaagt ggggctggcg actagcagcc ctgccacgtc
     4621 acagactgga cctggtatct gcatgaatgc taactttaac cagacccacc caggcctcct
     4681 caatagtaac tctggccata gcttaattaa tcaggcttca caagggcagg cgcaagtcat
     4741 gaatggatct cttggggctg ctggcagagg aaggggagct ggaatgccgt accctactcc
     4801 agccatgcag ggcgcctcga gcagcgtgct ggctgagacc ctaacgcagg tttccccgca
     4861 aatgactggt cacgcgggac tgaacaccgc acaggcagga ggcatggcca agatgggaat
     4921 aactgggaac acaagtccat ttggacagcc ctttagtcaa gctggagggc agccaatggg
     4981 agccactgga gtgaaccccc agttagccag caaacagagc atggtcaaca gtttgcccac
     5041 cttccctaca gatatcaaga atacttcagt caccaacgtg ccaaatatgt ctcagatgca
     5101 aacatcagtg ggaattgtac ccacacaagc aattgcaaca ggccccactg cagatcctga
     5161 aaaacgcaaa ctgatacagc agcagctggt tctactgctt catgctcata agtgtcagag
     5221 acgagagcaa gcaaacggag aggttcgggc ctgctcgctc ccgcattgtc gaaccatgaa
     5281 aaacgttttg aatcacatga cgcattgtca ggctgggaaa gcctgccaag ccatcctggg
     5341 gtctccagct agtggaattc aaaacacaat tggttctgtt ggcacagggc aacagaatgc
     5401 cacttcttta agtaacccaa atcccataga ccccagctcc atgcagcgag cctatgctgc
     5461 tctcggactc ccctacatga accagcccca gacgcagctg cagcctcagg ttcctggcca
     5521 gcaaccagca cagcctcaaa cccaccagca gatgaggact ctcaaccccc tgggaaataa
     5581 tccaatgaac attccagcag gaggaataac aacagatcag cagcccccaa acttgatttc
     5641 agaatcagct cttccgactt ccctgggggc cacaaaccca ctgatgaacg atggctccaa
     5701 ctctggtaac attggaaccc tcagcactat accaacagca gctcctcctt ctagcaccgg
     5761 tgtaaggaaa ggctggcacg aacatgtcac tcaggacctg cggagccatc tagtgcataa
     5821 actcgtccaa gccatcttcc caacacctga tcccgcagct ctaaaggatc gccgcatgga
     5881 aaacctggta gcctatgcta agaaagtgga aggggacatg tacgagtctg ccaacagcag
     5941 ggatgaatat tatcacttat tagcagagaa aatctacaag atacaaaaag aactagaaga
     6001 aaaacggagg tcgcgtttac ataaacaagg catcttgggg aaccagccag ccttaccagc
     6061 cccgggggct cagccccctg tgattccaca ggcacaacct gtgagacctc caaatggacc
     6121 cctgtccctg ccagtgaatc gcatgcaagt ttctcaaggg atgaattcat ttaaccccat
     6181 gtccttgggg aacgtccagt tgccacaagc acccatggga cctcgtgcag cctccccaat
     6241 gaaccactct gtccagatga acagcatggg ctcagtgcca gggatggcca tttctccttc
     6301 ccgaatgcct cagcctccga acatgatggg tgcacacacc aacaacatga tggcccaggc
     6361 gcccgctcag agccagtttc tgccacagaa ccagttcccg tcatccagcg gggcgatgag
     6421 tgtgggcatg gggcagccgc cagcccaaac aggcgtgtca cagggacagg tgcctggtgc
     6481 tgctcttcct aaccctctca acatgctggg gcctcaggcc agccagctac cttgccctcc
     6541 agtgacacag tcaccactgc acccaacacc gcctcctgct tccacggctg ctggcatgcc
     6601 atctctccag cacacgacac cacctgggat gactcctccc cagccagcag ctcccactca
     6661 gccatcaact cctgtgtcgt cttccgggca gactcccacc ccgactcctg gctcagtgcc
     6721 cagtgctacc caaacccaga gcacccctac agtccaggca gcagcccagg cccaggtgac
     6781 cccgcagcct caaaccccag ttcagccccc gtctgtggct acccctcagt catcgcagca
     6841 acagccgacg cctgtgcacg cccagcctcc tggcacaccg ctttcccagg cagcagccag
     6901 cattgataac agagtcccta ccccctcctc ggtggccagc gcagaaacca attcccagca
     6961 gccaggacct gacgtacctg tgctggaaat gaagacggag acccaagcag aggacactga
     7021 gcccgatcct ggtgaatcca aaggggagcc caggtctgag atgatggagg aggatttgca
     7081 aggagcttcc caagttaaag aagaaacaga catagcagag cagaaatcag aaccaatgga
     7141 agtggatgaa aagaaacctg aagtgaaagt agaagttaaa gaggaagaag agagtagcag
     7201 taacggcaca gcctctcagt caacatctcc ttcgcagccg cgcaaaaaaa tctttaaacc
     7261 agaggagtta cgccaggccc tcatgccaac cctagaagca ctgtatcgac aggacccaga
     7321 gtcattacct ttccggcagc ctgtagatcc ccagctcctc ggaattccag actattttga
     7381 catcgtaaag aatcccatgg acctctccac catcaagcgg aagctggaca cagggcaata
     7441 ccaagagccc tggcagtacg tggacgacgt ctggctcatg ttcaacaatg cctggctcta
     7501 taatcgcaag acatcccgag tctataagtt ttgcagtaag cttgcagagg tctttgagca
     7561 ggaaattgac cctgtcatgc agtcccttgg atattgctgt ggacgcaagt atgagttttc
     7621 cccacagact ttgtgctgct atgggaagca gctgtgtacc attcctcgcg atgctgccta
     7681 ctacagctat cagaataggt atcatttctg tgagaagtgt ttcacagaga tccagggcga
     7741 gaatgtgacc ctgggtgacg acccttcaca gccccagacg acaatttcaa aggatcagtt
     7801 tgaaaagaag aaaaatgata ccttagaccc cgaacctttc gttgattgca aggagtgtgg
     7861 ccggaagatg catcagattt gcgttctgca ctatgacatc atttggcctt caggttttgt
     7921 gtgcgacaac tgcttgaaga aaactggcag acctcgaaaa gaaaacaaat tcagtgctaa
     7981 gaggctgcag accacaagac tgggaaacca cttggaagac cgagtgaaca aatttttgcg
     8041 gcgccagaat caccctgaag ccggggaggt ttttgtccga gtggtggcca gctcagacaa
     8101 gacggtggag gtcaagcccg ggatgaagtc acggtttgtg gattctgggg aaatgtctga
     8161 atctttccca tatcgaacca aagctctgtt tgcttttgag gaaattgacg gcgtggatgt
     8221 ctgctttttt ggaatgcacg tccaagaata cggctctgat tgcccccctc caaacacgag
     8281 gcgtgtgtac atttcttatc tggatagtat tcatttcttc cggccacgtt gcctccgcac
     8341 agccgtttac catgagatcc ttattggata tttagagtat gtgaagaaat tagggtatgt
     8401 gacagggcac atctgggcct gtcctccaag tgaaggagat gattacatct tccattgcca
     8461 cccacctgat caaaaaatac ccaagccaaa acgactgcag gagtggtaca aaaagatgct
     8521 ggacaaggcg tttgcagagc ggatcatcca tgactacaag gatattttca aacaagcaac
     8581 tgaagacagg ctcaccagtg ccaaggaact gccctatttt gaaggtgatt tctggcccaa
     8641 tgtgttagaa gagagcatta aggaactaga acaagaagaa gaggagagga aaaaggaaga
     8701 gagcactgca gccagtgaaa ccactgaggg cagtcagggc gacagcaaga atgccaagaa
     8761 gaagaacaac aagaaaacca acaagaacaa aagcagcatc agccgcgcca acaagaagaa
     8821 gcccagcatg cccaacgtgt ccaatgacct gtcccagaag ctgtatgcca ccatggagaa
     8881 gcacaaggag gtcttcttcg tgatccacct gcacgctggg cctgtcatca acaccctgcc
     8941 ccccatcgtc gaccccgacc ccctgctcag ctgtgacctc atggatgggc gcgacgcctt
     9001 cctcaccctc gccagagaca agcactggga gttctcctcc ttgcgccgct ccaagtggtc
     9061 cacgctctgc atgctggtgg agctgcacac ccagggccag gaccgctttg tctacacctg
     9121 caacgagtgc aagcaccacg tggagacgcg ctggcactgc actgtgtgcg aggactacga
     9181 cctctgcatc aactgctata acacgaagag ccatgcccat aagatggtga agtgggggct
     9241 gggcctggat gacgagggca gcagccaggg cgagccacag tcaaagagcc cccaggagtc
     9301 acgccggctg agcatccagc gctgcatcca gtcgctggtg cacgcgtgcc agtgccgcaa
     9361 cgccaactgc tcgctgccat cctgccagaa gatgaagcgg gtggtgcagc acaccaaggg
     9421 ctgcaaacgc aagaccaacg ggggctgccc ggtgtgcaag cagctcatcg ccctctgctg
     9481 ctaccacgcc aagcactgcc aagaaaacaa atgccccgtg cccttctgcc tcaacatcaa
     9541 acacaagctc cgccagcagc agatccagca ccgcctgcag caggcccagc tcatgcgccg
     9601 gcggatggcc accatgaaca cccgcaacgt gcctcagcag agtctgcctt ctcctacctc
     9661 agcaccgccc gggaccccca cacagcagcc cagcacaccc cagacgccgc agccccctgc
     9721 ccagccccaa ccctcacccg tgagcatgtc accagctggc ttccccagcg tggcccggac
     9781 tcagcccccc accacggtgt ccacagggaa gcctaccagc caggtgccgg cccccccacc
     9841 cccggcccag ccccctcctg cagcggtgga agcggctcgg cagatcgagc gtgaggccca
     9901 gcagcagcag cacctgtacc gggtgaacat caacaacagc atgcccccag gacgcacggg
     9961 catggggacc ccggggagcc agatggcccc cgtgagcctg aatgtgcccc gacccaacca
    10021 ggtgagcggg cccgtcatgc ccagcatgcc tcccgggcag tggcagcagg cgccccttcc
    10081 ccagcagcag cccatgccag gcttgcccag gcctgtgata tccatgcagg cccaggcggc
    10141 cgtggctggg ccccggatgc ccagcgtgca gccacccagg agcatctcac ccagcgctct
    10201 gcaagacctg ctgcggaccc tgaagtcgcc cagctcccct cagcagcaac agcaggtgct
    10261 gaacattctc aaatcaaacc cgcagctaat ggcagctttc atcaaacagc gcacagccaa
    10321 gtacgtggcc aatcagcccg gcatgcagcc ccagcctggc ctccagtccc agcccggcat
    10381 gcaaccccag cctggcatgc accagcagcc cagcctgcag aacctgaatg ccatgcaggc
    10441 tggcgtgccg cggcccggtg tgcctccaca gcagcaggcg atgggaggcc tgaaccccca
    10501 gggccaggcc ttgaacatca tgaacccagg acacaacccc aacatggcga gtatgaatcc
    10561 acagtaccga gaaatgttac ggaggcagct gctgcagcag cagcagcaac agcagcagca
    10621 acaacagcag caacagcagc agcagcaagg gagtgccggc atggctgggg gcatggcggg
    10681 gcacggccag ttccagcagc ctcaaggacc cggaggctac ccaccggcca tgcagcagca
    10741 gcagcgcatg cagcagcatc tccccctcca gggcagctcc atgggccaga tggcggctca
    10801 gatgggacag cttggccaga tggggcagcc ggggctgggg gcagacagca cccccaacat
    10861 ccagcaagcc ctgcagcagc ggattctgca gcaacagcag atgaagcagc agattgggtc
    10921 cccaggccag ccgaacccca tgagccccca gcaacacatg ctctcaggac agccacaggc
    10981 ctcgcatctc cctggccagc agatcgccac gtcccttagt aaccaggtgc ggtctccagc
    11041 ccctgtccag tctccacggc cccagtccca gcctccacat tccagcccgt caccacggat
    11101 acagccccag ccttcgccac accacgtctc accccagact ggttcccccc accccggact
    11161 cgcagtcacc atggccagct ccatagatca gggacacttg gggaaccccg aacagagtgc
    11221 aatgctcccc cagctgaaca cccccagcag gagtgcgctg tccagcgaac tgtccctggt
    11281 cggggacacc acgggggaca cgctagagaa gtttgtggag ggcttgtagt aatgaactag
    11341 tccgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaact
    11401 agacgaggca cagataggtc gacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
    11461 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
    11521 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
    11581 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
    11641 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
    11701 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
    11761 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
    11821 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
    11881 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
    11941 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
    12001 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
    12061 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
    12121 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
    12181 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
    12241 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
    12301 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
    12361 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
    12421 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    12481 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
    12541 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
    12601 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    12661 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
    12721 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
    12781 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
    12841 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
    12901 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
    12961 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
    13021 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
    13081 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
    13141 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
    13201 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
    13261 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
    13321 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
    13381 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
    13441 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
    13501 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
    13561 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
    13621 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
    13681 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
    13741 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
    13801 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
    13861 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
    13921 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
    13981 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
    14041 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
    14101 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
    14161 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    14221 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    14281 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    14341 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    14401 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    14461 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    14521 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    14581 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    14641 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    14701 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    14761 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    14821 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    14881 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    14941 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    15001 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    15061 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    15121 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    15181 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    15241 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    15301 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    15361 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    15421 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    15481 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
    15541 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
//
