LOCUS       sequence-397934-       13399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0912
FEATURES             Location/Qualifiers
     source          1..13399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    9256..9844
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9309..9329)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9727..9738)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9916..10149
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10227..10348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10264..10283)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10318..10337
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10388..10523
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10543..10562)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10544..10562)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10572..10589)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10572..10588)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10581..10603)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10730..11185
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10817..10836)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11027..11048
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11211..11315
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11316..11384,11385..12176)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11534..11553)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12347..12935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12836..12855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13089..13106
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13223..13244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13259..13264,13265..13282,13283..13289)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13297..13313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13302..13324
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13321..13337
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13321..13337
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13356..13376
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13358..13376
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag gttccagaac ccacctgccc
     4141 tgcccctcct gcgagggacc agccagctcc cactcctggc cctccagggg ccccaggtgg
     4201 ccaggcctca cctcacctga ccctgggccc tgtccttctg ccgccagagc agggcctggc
     4261 cccccccact gtgttcctga aggccctgcc catcccactg taccacacgg tgcctcccgg
     4321 gggcctccag ccccgcgccc cgctagtgac gggcagccta gatgggggca acgtgccctt
     4381 catactcagc cctgtgctgc agcctgaagg gcctggcccc acccaggtgg ggaagccggc
     4441 ggcccctacg ctgacggtga acatcgtggg cactctgcct gtcctgtcgc cgggcctggg
     4501 ccccacgctg ggcagcccag gcaaggtgcg gaatgcgggc aagtacctgt gtccgcactg
     4561 tggtcgcgac tgcctgaagc ccagtgttct agagaagcac atccggtccc acacgggtga
     4621 gaggcccttc ccgtgtgcca cctgcggcat cgcctttaag acccagagca atctctacaa
     4681 gcacaggcgg acgcagacgc acctcaacaa ctcccggctg tcctcagagt ccgagggcgc
     4741 cgggggcggc ctcctggagg aaggggacaa ggccggagag ccccccagac cagagggcag
     4801 gggcgagagc aggtgccagg ggatgcacga aggcgcctcg gagagacccc tttctccggg
     4861 tgcccacgtg cccctacttg ccaagaacct ggatgtgagg accgaagctg ctccctgtcc
     4921 agggtccgca tttgccgaca gagaggctcc ttgggactct gcccccatgg cgtcacctgg
     4981 gctcccagcg gccagcacac aaccctggcg taagttgcca gagcagaagt cgccgaccgc
     5041 cgggaagccg tgcgccctgc agcggcagca ggcgacggca gcggagaagc cctgggatgc
     5101 caaggccccc gagggccggc tgcggaagtg tgagagcacc gactcggggt acctgtcgcg
     5161 ctccgacagc gcggagcagc cgcatgcgcc ctgcagcccc ctgcacagcc tttcggagca
     5221 cagcgccgag tccgaggggg agggcggccc gggcccgggg ccaggggtcg caggggccga
     5281 gcccggggcg cgagaagccg gcctggagct ggagaagaag cggctggagg agcgcatcgc
     5341 ccagctcatc tcccacaacc aggcggtggt ggacgatgcc cagctggaca acgtgcggcc
     5401 ccggaagacc gggctgtcca aacagggcag catcgacctg cccacgccct acacctacaa
     5461 ggactccttc cactttgaca tccgcgcgct ggagccaggc cgtaggaggg ccccgggccc
     5521 cgtgcgctcc acctggacgc ccccagacaa gtctcggccc ctcttcttcc actccgtccc
     5581 cactcagctc tccaccaccg tggaatgtgt ccccgtcacc aggagcaact cgctgccctt
     5641 cgtcgagggc tccaggacgt ggctggagcc cagggagccc cgggacccct ggtccaggac
     5701 gcagaagcct ctgagcccca ggcccggccc agcccgcctg ggctgccgct cgggactaag
     5761 ctcgactgac gttcccagtg ggcatccccg ggccctggtc agacaggccg cggtggagga
     5821 cctgccaggc acccccattg gcgatgccct ggtgcccgca gaggacacag acgcaaagag
     5881 aactgctgcg cgggaggcca tggccggcaa gggcagagcg ggcggcagga agtgcggcca
     5941 gagaaggctg aagatgttct cccaggagaa gtggcaggtg tacggggatg agacgttcaa
     6001 aaggatctac cagaaaatga aagccagtcc ccatggaggc aagaaagcca gggaggtggg
     6061 aatgggcagt ggggcagaac tgggctttcc tctgcagaaa gaggcagcag ggagctcagg
     6121 cacagtcccc acccaagaca ggaggacccc tgtccatgag gacatatccg caggggcaac
     6181 gccagagcct tggggaaatc caccagccct ggaggcctcc ttggtgactg aacccactaa
     6241 gcatggggag acggtggcca ggagaggaga cagtgaccga cccagggtgg aagaggctgt
     6301 gtcatcccct gcactgggtg gcagagacag tccctgttca ggcagtagga gccccctggt
     6361 ctctccaaat gggaggctgg aactggggtg gcagatgccc ccagcacctg gccccctcaa
     6421 agggggtgat gtagaggctc ccaggccagt ttggccggac cccaagctgg aaggaggtgc
     6481 ccgaggtgtg ggggatgttc aggagacctg cctgtgggcc cagactgtcc tgagatggcc
     6541 cagcaggggc tcaggggagg acaagctccc ctcagagagg aagaagctga aagtggagga
     6601 cctgcacagc tggaagcaac cagagcctgt gagcgcagag accccaggtg ggcccacgca
     6661 gcctgcctct ttgtcatccc agaagcagga tgccgatccc ggggaggtgc cagggggctc
     6721 aaaggagagt gccaggcagg tgggcgagcc tctggagtcc tctggagcct ccttggctgc
     6781 tgcttctgtt gccctgaaga gggtggggcc aagggacaag gctaccccac tgcatcctgc
     6841 agccccagcc cccgcagagc acccctcgct ggccacccca cctcaggctc ctagagtgct
     6901 ctctgccctg gcagataatg ccttttcccc caagtacctc ctcaggttac ctcaggcaga
     6961 gaccccctta ccactgccca ttccctgggg accaaggcac agccaggact ctctctgcag
     7021 cagtgggtgg cctgaagaac gggcatcatt tgttgggtca ggactgggga cccctctttc
     7081 tcccagccca gcctcaggcc cctccccagg tgaggcggac agcatcctgg aggaccccag
     7141 ctgttccagg ccacaggatg ggagaaaagg ggcacagttg gggggggaca agggggacag
     7201 gatggccact agcaggccag cagccaggga gttgcccatc tcagcaccag gggctcccag
     7261 ggaggctacc tcctccccgc ccactccaac gtgtgaggca cacttagttc aggacatgga
     7321 gggtgacagc caccgtatcc atcgcctctg catgggcagc actttggcaa gggccaggct
     7381 ctctggggat gtcctgaatc cctgggtacc caactgggag ctgggggagc ctcctgggaa
     7441 tgccccagaa gatccttctt cagggcccct ggtgggcccc gacccgtgtt cccccctcca
     7501 gcctggctcc ttcctcactg ccctcactcg gcctcagggt gtgcccccag gctggccaga
     7561 gctggccttg tcttcccact cagggacgtc ccggagccac agcacccgca gtccccacag
     7621 cacccaaaac ccctttccct cactgaaggc tgagccgcgg ctcacgtggt gttgcctgag
     7681 ccgcagtgtc cctctgcccg cggagcagaa ggcaaaggcg gcatctgtgt acttggcggt
     7741 gcactttcct ggtagcagcc tccgagatga gggtcccaat ggccctcctg ggagcaatgg
     7801 aggatggacc tggacaagcc ctggagaagg agggccggcg cagatgtcca agttctccta
     7861 cccaacagtc ccaggggtga tgccccagca ccaggtgtct gagccagaat ggaagaaagg
     7921 cctgccttgg agggcaaaga tgtctcgtgg gaacagcaag cagagaaaac tgaagatcaa
     7981 ccctaaaagg tacaaaggga atttcttgca gagctgtgtt cagctgagag ccagtagact
     8041 tcgcacacca acctgggtgc gaagaagaag ccgccaccct cccgcacttg agggactgaa
     8101 gccatgcagg acccctgggc agacctcttc agaaatagca ggtctgaatc tgcaagagga
     8161 gccatcttgt gccacctcag aatcacctcc ttgttgtggg aaggaagaga agaaggaagg
     8221 tgactgcaga caaaccttag gaaccctctc tcttggtaca agttcaagaa ttgtcaggga
     8281 aatggacaaa cgaactgtga aggatatttc tccatctgct ggtgagcatg gtgactgtac
     8341 tactcacagc actgctgcca catcaggatt atctctgcaa tctgacacct gcctggcagt
     8401 ggttaatgac gtgcctctac cccctggcaa aggtcttgac cttgggttgc tggagactca
     8461 gctgctggcc tcccaggatt cagtctcaac agatcccaaa ccatacatct tctcagatgc
     8521 tcaaaggcct tcttcctttg ggtccaaagg aacttttccc caccatgaca ttgctacctc
     8581 tgtggctgcc gtttgtattt ctctgccagt gagaacagat cacatagccc aggaaattca
     8641 cagtgctgaa tcacgagacc acagccagac tgcagggagg actctgacat caagctcccc
     8701 agacagcaaa gtcacagaag agggcagagc acagaccctc ttgccaggga gaccttcatc
     8761 tggacaaaga atttcagatt cggttccact ggagtcaact gaaaaaactc atcttgaaat
     8821 accagcttca ggaccaagtt cagctagttc acaccacaag gaagggagac acaagacgtt
     8881 ttttccttcc agaggccagt atgggtgtgg ggaaatgact gtcccctgcc cctctttagg
     8941 aagtgacggt aggaaacgtc aggtatctgg attaatcact cggaaagatt ctgtggttcc
     9001 ttctaagcca gagcagccca tagaaattcc tgaagcccct tctaaatccc tcaagaagag
     9061 gagtctggaa ggaatgagaa agcaaactcg agtagagttc agtgacacca gcagcgacga
     9121 tgaagaccga ttagttatag aaatatagta atgaactagt ccgtaacttg aaagtatttc
     9181 gatttcttgg ctttatatat cttgtggaaa ggacgacacc catatcccta cgatagcagc
     9241 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     9301 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     9361 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     9421 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     9481 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     9541 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     9601 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     9661 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     9721 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     9781 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     9841 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     9901 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     9961 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
    10021 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
    10081 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
    10141 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
    10201 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
    10261 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    10321 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
    10381 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    10441 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
    10501 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
    10561 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
    10621 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
    10681 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
    10741 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
    10801 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
    10861 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
    10921 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
    10981 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
    11041 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
    11101 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
    11161 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
    11221 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    11281 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    11341 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    11401 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    11461 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    11521 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
    11581 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    11641 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    11701 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    11761 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    11821 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    11881 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    11941 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    12001 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    12061 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    12121 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    12181 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    12241 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    12301 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    12361 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    12421 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    12481 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    12541 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    12601 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    12661 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    12721 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    12781 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    12841 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    12901 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    12961 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    13021 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    13081 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    13141 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    13201 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    13261 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    13321 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
    13381 aaaagctgga gctgcaagc
//
