LOCUS       sequence-397942-       10750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10750)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1377
FEATURES             Location/Qualifiers
     source          1..10750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6607..7195
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6660..6680)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7078..7089)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7267..7500
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7578..7699
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7615..7634)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7669..7688
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7739..7874
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7894..7913)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7895..7913)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7923..7940)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7923..7939)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7932..7954)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8081..8536
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8168..8187)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8378..8399
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8562..8666
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8667..8735,8736..9527)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8885..8904)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9698..10286
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10187..10206
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10440..10457
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10574..10595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10610..10615,10616..10633,10634..10640)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10648..10664
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10653..10675
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10672..10688
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10672..10688
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10707..10727
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10709..10727
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaac attgacgaca aactggaagg
     4141 attgtttctt aaatgtggcg gcatagacga aatgcagtct tccaggacaa tggttgtaat
     4201 gggtggagtg tctggccagt ctactgtgtc tggagagcta caggattcag tacttcaaga
     4261 tcgaagtatg cctcaccagg agatccttgc tgcagatgaa gtgttacaag aaagtgaaat
     4321 gagacaacag gatatgatat cacatgatga actcatggtc catgaggaga cagtgaaaaa
     4381 tgatgaagag cagatggaaa cacatgaaag acttcctcaa ggactacagt atgcacttaa
     4441 tgtccctata agcgtaaagc aggaaattac ttttactgat gtatctgagc aactgatgag
     4501 agacaaaaaa caaatcagag agccagtaga cttacagaaa aagaagaagc ggaaacaacg
     4561 ttctcccgca aaaatcctta caataaatga ggatggatca cttggtttga aaacccctaa
     4621 atctcacgtt tgtgagcact gcaatgctgc ctttagaacg aactatcact tacagagaca
     4681 tgtcttcatt catacaggtg aaaaaccatt tcaatgtagt caatgtgaca tgcgtttcat
     4741 acagaagtac ctgcttcaga gacatgagaa gattcatact ggtgaaaaac catttcgctg
     4801 tgatgaatgt ggtatgagat tcatacaaaa atatcatatg gaaaggcata agagaactca
     4861 tagtggagaa aaaccttacc agtgtgaata ctgtttacag tatttttcca gaacagatcg
     4921 tgtattgaaa cataaacgta tgtgccatga aaatcatgac aaaaaactaa atagatgtgc
     4981 catcaaaggt ggccttctga catctgagga agattctggc ttttctacat caccaaaaga
     5041 caactcactg ccaaaaaaga aaaggcagaa aacggagaaa aaatcatctg gaatggacaa
     5101 agagagtgct ttggacaaat ctgacctgaa aaaagacaaa aatgattact tgcctcttta
     5161 ttcttcaagt actaaagtaa aagatgagta tatggttgca gaatatgctg ttgaaatgcc
     5221 acattcgtca gttgggggct cgcatttaga agatgcgtca ggagaaatac acccacctaa
     5281 gttagttctc aaaaaaatta atagtaagag aagtctgaaa cagccactgg agcaaaatca
     5341 aacaatttca cctttatcca catatgaaga gagcaaagtt tcaaagtatg cttttgaact
     5401 tgtggataaa caggctttac tggactcaga aggcaatgct gacattgatc aggttgataa
     5461 tttgcaggag gggcccagta aacctgtgca tagtagtact aattatgatg atgccatgca
     5521 gtttttgaag aagaagcggt atcttcaagc agcaagtaac aacagcaggg aatatgcgct
     5581 gaatgtgggt accatagctt ctcagccttc tgtaacacaa gcagctgtgg caagtgtcat
     5641 tgatgaaagt accacggcat ccatattaga gtcacaggca ctgaatgtgg agattaagag
     5701 taatcatgac aaaaatgtta ttccagatga ggtactgcag actctgttgg atcattattc
     5761 ccacaaagct aatggacagc atgagatatc cttcagtgtt gcagatactg aagtgacttc
     5821 tagcatatca ataaattctt cagaagtacc agaggtcacc ccgtcagaga atgttggatc
     5881 aagctcccaa gcatcctcat cagataaagc caacatgttg caggaatact ccaagtttct
     5941 gcagcaggct ttggacagaa ctagccaaaa tgatgcctat ttgaatagcc cgagccttaa
     6001 ctttgtgact gataaccaga ccctcccaaa tcagccagca ttctcttcca tagacaagca
     6061 ggtctatgcc accatgccca tcaatagctt tcgatcagga atgaattctc cactaagaac
     6121 aactccagat aagtcccact ttggactaat agttggtgat tcacagcact catttccctt
     6181 ttcaggtgat gagacaaacc atgcttctgc cacatcaaca caggactttc tggatcaagt
     6241 gacttctcag aagaaagctg aggcccagcc tgtccaccaa gcttaccaaa tgagctcctt
     6301 tgaacagccc ttccgtgctc cctatcatgg atcaagagct ggaatagcta ctcaatttag
     6361 cactgccaat ggacaggtga accttcgggg accagggaca agtgctgaat tttcagaatt
     6421 tcccttggtg aatgtaaatg ataatagagc tgggatgaca tcttcacctg atgccacaac
     6481 tggccagact tttggctagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     6541 gctttatata tcttgtggaa aggacgagcc gctgcgcgtc actccatcta cacgcgttaa
     6601 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6661 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6721 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6781 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6841 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6901 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6961 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     7021 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7081 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7141 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     7201 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7261 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     7321 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7381 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7441 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7501 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7561 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7621 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7681 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     7741 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7801 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     7861 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     7921 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     7981 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     8041 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     8101 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     8161 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     8221 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     8281 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     8341 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     8401 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     8461 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     8521 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8581 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8641 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8701 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8761 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     8821 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     8881 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     8941 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     9001 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     9061 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     9121 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     9181 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     9241 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     9301 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     9361 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9421 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9481 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9541 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9601 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9661 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9721 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     9781 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     9841 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     9901 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     9961 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    10021 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    10081 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    10141 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    10201 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    10261 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    10321 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    10381 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    10441 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    10501 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    10561 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    10621 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    10681 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    10741 agctgcaagc
//
