LOCUS       sequence-398000-       11151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre reporter, which switches from membraneous mTurquoise to nuclear 
            RFP upon Cre recombinase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11151)
  AUTHORS   Vizoso M, E J Pritchard C, Bombardelli L, van den Broek B, 
            Krimpenfort P, Beijersbergen RL, Jalink K, van Rheenen J
  TITLE     A doxycycline- and light-inducible Cre recombinase mouse model for 
            optogenetic genome editing.
  JOURNAL   Nat Commun. 2022 Oct 28;13(1):6442. doi: 10.1038/s41467-022-33863-z.
  PUBMED    36307419
REFERENCE   2  (bases 1 to 11151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-28"; volume: "13(1):6442. doi"; pages: " 
            10.1038/s41467-022-33863-z"
COMMENT     Sequence Label: 
            EF1-alpha-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP
FEATURES             Location/Qualifiers
     source          1..11151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(192..211)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     539..560
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(606..1298)
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKVV
                     EGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTAT
                     QDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALKL
                     VGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYCD
                     LPSKLGHR"
     CDS             complement(1302..1322)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      1325..1334
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    1357..1390
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    1441..1474
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    1499..2087
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1552..1572)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1970..1981)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2090..2106)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2091..2107)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2612..2792
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2818..2835)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2824..3048
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3094..3522
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3181..3200)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3391..3412
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3531..3551)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3536..3865
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3716..3851
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3778..3797
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3913..3960
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3979..4353
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4483..4604
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4520..4539)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4574..4593
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4653..4669)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4653..4669)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4666..4688)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4677..4693
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4701..4707,4708..4725,4726..4731))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4746..4767
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4884..4901)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5055..5643)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5135..5154)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5814..6605,6606..6674))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6437..6456
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6675..6779)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6854..6873)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7045..7424
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7426..7624
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7579..7599
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7642..7822
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7869..7994
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8487..8720
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8905..8949
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9247..9364
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9557..10735
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9788..10726
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10683..10703
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(10766..10799)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(10850..10883)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      10905..10914
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(11040..11042,11043..11151,1..605)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(11082..11103)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
       61 ccaccctcgt gaccaccctg tcctggggcg tgcagtgctt cgcccgctac cccgaccaca
      121 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
      181 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
      241 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
      301 ggcacaagct ggagtacaac tactttagcg acaacgtcta tatcaccgcc gacaagcaga
      361 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc
      421 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
      481 accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca
      541 tggtcctgct ggagttcgtg acagccgccg ggatcactct cggcatggac gagctgtaca
      601 agtaatcatc tgtgccccag tttgctaggg aggtcgcagt acttggccac agccatctcg
      661 tgctgctcga cgtaggtctc tttgtcggcc tccttgattc tttccagtct gtggtccacg
      721 aagtggaagc cgggcatctt gaggttctta gcgggtttct tggatctgta tgtggtcttg
      781 aaggagcagt gcaggtagcc cccgcccacg agcttcaggg ccatctggct gtggcctctc
      841 aggccgccgt cagcggggta cagcatctcg gtgttggcct cccagccgcg tgttttcttc
      901 tgcatcacag ggccgttgga tgggaagttc accccgttga tcttgacgtt gtagatgatg
      961 cagccgttct ggaagctggt gtcctgggta gcggtcagca cgcccccgtc ttcgtatgtg
     1021 gtgattctct cccatgtgaa gccctcaggg aaggactgct taaagaagtc ggggatgccc
     1081 tgggtgtggt tgatgaaggc tttgctgccg tacatgaagc tggtagccag gatgtcgaag
     1141 gcgaagggga gagggccgcc ctcgaccacc ttgatcttca tggtctgggt gccctcgtag
     1201 ggcttgcctt cgccctcgga tgtgcacttg aagtggtggt tgttcacggt gccctccatg
     1261 tacagcttca tgtgcatgtt ctccttgatc agctcgctcg aaacctttct cttcttcttt
     1321 gggcccatgg tggcggtggc gaccggtagc gctagcataa cttcgtataa agtatcctat
     1381 acgaagttat ttgccttaac ccagaaatta tcactgttat tctttagaat ggtgcaaaga
     1441 ataacttcgt ataatgtatg ctatacgaag ttatgaattc gatatcaagc ttatcggtaa
     1501 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     1561 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     1621 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     1681 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     1741 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     1801 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     1861 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     1921 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     1981 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     2041 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     2101 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     2161 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     2221 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     2281 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     2341 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     2401 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     2461 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     2521 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     2581 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     2641 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     2701 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     2761 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     2821 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     2881 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     2941 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     3001 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     3061 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     3121 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     3181 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3241 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     3301 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     3361 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3421 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     3481 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     3541 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3601 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3661 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     3721 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     3781 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     3841 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     3901 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     3961 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     4021 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     4081 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     4141 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     4201 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     4261 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     4321 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
     4381 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     4441 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     4501 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4561 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     4621 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     4681 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     4741 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     4801 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     4861 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4921 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4981 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5041 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5101 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5161 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5221 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5281 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5341 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5401 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5461 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     5521 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5581 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5641 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5701 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5761 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5821 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5881 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5941 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6001 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6061 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     6121 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     6181 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     6241 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     6301 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     6361 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     6421 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     6481 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     6541 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     6601 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6661 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6721 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6781 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     6841 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     6901 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     6961 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     7021 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     7081 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     7141 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     7201 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     7261 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     7321 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     7381 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     7441 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     7501 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     7561 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     7621 gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7681 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7741 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7801 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     7861 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     7921 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     7981 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     8041 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     8101 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     8161 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     8221 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     8281 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     8341 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     8401 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     8461 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     8521 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     8581 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     8641 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     8701 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     8761 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     8821 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     8881 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     8941 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     9001 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     9061 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     9121 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     9181 cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat
     9241 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     9301 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     9361 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattagct agctttaacg
     9421 atgtgctgag ggcagtcccc gatctcgtgc ttgcccaaat cccaacgcgt aagctttgca
     9481 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
     9541 gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     9601 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
     9661 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     9721 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     9781 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
     9841 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg
     9901 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
     9961 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
    10021 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
    10081 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
    10141 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
    10201 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
    10261 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
    10321 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
    10381 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
    10441 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
    10501 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
    10561 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
    10621 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
    10681 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggtac
    10741 cggatcctct agagtcgact ccggaataac ttcgtatagg atactttata cgaagttatg
    10801 cagaatggta gctggattgt agctgctatt agcaatatga aacctcttaa taacttcgta
    10861 tagcatacat tatacgaagt tatggcgcgc cttatctaga tttagccacc atgggttgct
    10921 gtttctccaa gaccgcagcg aagggagaag ccgccgcgga gaggcctggg gaggcggctg
    10981 tggcctcgtc gccttccaaa gcgaacggac aggagaatgg ccacgtgaag gtaggatccg
    11041 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
    11101 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc a
//
