LOCUS       sequence-398002-       13843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetically encoded voltage indicator (GEVI) JEDI-1P under the HuC 
            promoter for pan neuronal expression in zebrafish; JEDI-1P sequence 
            codon-optimized for zebrafish expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13843)
  AUTHORS   Lu X, Wang Y, Liu Z, Gou Y, Jaeger D, St-Pierre F
  TITLE     Widefield imaging of rapid pan-cortical voltage dynamics with an 
            indicator evolved for one-photon microscopy.
  JOURNAL   Nat Commun. 2023 Oct 12;14(1):6423. doi: 10.1038/s41467-023-41975-3.
  PUBMED    37828037
REFERENCE   2  (bases 1 to 13843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-10-12"; volume: "14(1):6423. doi"; pages: " 
            10.1038/s41467-023-41975-3"
COMMENT     Sequence Label: pHUC-JEDI-1P
FEATURES             Location/Qualifiers
     source          1..13843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3403..3412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4739..4873
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4763..4782)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4817..4836
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(5312..5499,5500..5511)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5545..5561)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5545..5561)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5598..5618)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5598..5616)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5637..5653)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5637..5653)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5650..5672)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5661..5677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5685..5691,5692..5709,5710..5715))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5730..5751
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5868..5885)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6039..6627)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6119..6138)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6798..7589,7590..7658))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7421..7440
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7659..7763)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7926..7947)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(8296..8307,8308..8470)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 acgattcgca aacgtgttta tttgtaaaga cgacatcaag agcttaatgt ccacttgaaa
       61 agaaataaat atgcccgcgg cgccaaggat attttgaatg tagtcgttca tggtgagcca
      121 ctcccgaatt cagcactctg gagagcgacc aaagaggaat agcggaatag caactacttg
      181 agccatttct ctccgtttga tggcgttcat gattaaaaat ataatcacgt gattttttat
      241 acatatataa atatatacat aaagtaacgc tttccttttg agaatacata aatatttatt
      301 cgcgtgataa atcaatgatc ttatatttat ttgcacgacc gaaattagcc aaattcacca
      361 attaaaaaaa aaataagcca acaaaaaaga agggtttatc cgttcaattt tgacttgtgt
      421 cctgttgttt tacagctgcc actgtgatcc cttaagctgc agtgagagtg tggctaatgc
      481 cttttgttta agataatcct gtaatctgtt accgaaacgg cctattgaca agccggcatt
      541 cacatttcag tcaggaggcc cgtccagaca tgtacattta tgaattacga atgataaaat
      601 taagatctgc attaggcgtc agaaatgtca cgaacaccca ttcatttccc acagccaata
      661 atggatcatg ctgggaagcg ctatcctcgc agtctcaaaa ttaatgttga catgttgcgt
      721 agagctacag attagatcag ccatatgctt tatgtgttta cctcagcgga tcttcagcaa
      781 gatgtgttta ttttaagaaa atggcttcct cgctgcctgc acaggggcat taaggaagtg
      841 acgtgagcgt cctcacagtc aaattacttt atctcatgct gctttcaggc ctctgcttat
      901 tttattatta ttattttaaa ttagggggaa tcacggtggc gttgtgggta gggtagcgcg
      961 atcaccttac agcaagaagg tcgctggttc gagctctggc tgggtcagtt ggcatttctg
     1021 tgtggagttt aaattgaatt gaaattgaat aaactaaatt ggccctagtg tatgtgtgtg
     1081 aatgtaagtg tgcatgggtg tttcccagtg ttgggtttga ctggaagagc atccgcttcg
     1141 taaaacatat gctggataag ttggcggttc atttcactgt ggtgaccgct gattaataaa
     1201 gggactaagc tgaaaatgaa atgaattaat acattttaaa tgagcttttg ctgacatgtt
     1261 tatgattaca aatagtgaaa ttgtatgtta atttacgata agtgcattgt tctaaattca
     1321 tttttatgta gcagctggtt tttcttccat ttgtgtcatt caacagaaca tttttctaaa
     1381 aaagtgaact taaattttct cctccctctg acaaggcttg atggtaattt cagccgactc
     1441 taattacaga ctcactaaaa ggaatcggtg cagtcattta tctttatcag cgcgctggtg
     1501 ggaaggtaat aggttttgct tatgagttgt ttgcgtgcga gtctgggtcc ttgtgtaggc
     1561 gatgctgatg cactgcatta aatattgagg gagaagtcgc tcaaaaatag caaagtacag
     1621 gctggggcag gaacgtcaaa gcccgctcag tggccacgtt tcttgtttcg taagtcctat
     1681 ttttaatagc gataattcag gctcagtaga aggaactcca gcactataag cagtctctcg
     1741 cctgtctcgg cgagttatgt attttgggat gaagtgctct tccacctcta cctctgctgt
     1801 gctggcgcca gtatctgggc tgtaagatag tgtacgtgtc agtctgtgtg gccgaagagc
     1861 ctgtgtgttg tgtaactcac tacattcgtc gctgtggatg gctgacttgc catggggagg
     1921 tttacttcag ttcacgaatc acattcgcca cgatcttgtc actttgtgtc aagactcgct
     1981 ctctgtgtgt caacagctct gctgtttgag gttgaaattc tgaacttgag atatatccca
     2041 ggatatatta gggctactgc caacggaaaa agcaaatggt gccatggcca tttgattgat
     2101 caaacaaaca cccttttgct tgggtgacag tgaaatgtca gatccatgtt tgtactgctt
     2161 taataacttg cctctctgag ctttctcctt gtcagtgtca atttgcctga gatataaatt
     2221 ccggttgccc cgaaagctta ttttattaaa aaaaagctaa catgcactca tacaacgtgc
     2281 gcacatacat gtgaggcttg ctaaaagtca agaagcaagc caatagcaag agtcgtcaga
     2341 ctgctgttac ctgggcaacc tggttagagg taaggctcaa ggcaagaata aatcattcca
     2401 ctgaagagac acagcgttac agcgagatga gagcaagagg cgatagtgaa gggagaacaa
     2461 aagaacagcc tacccacact agtgcagatc tacctgtttg agctactgat tgttcagttt
     2521 gattagatat cagacaattc tgctatctcc aattgcattg ctgcatgaat tcctagaaat
     2581 cattttaatc accaaaaact agtagggaca cttgtggcgt atatgattct gcctgttgat
     2641 tgtggggatc caacagaaag atcatgaatg cattgttaat taagtcggtg agacaccctg
     2701 ttccacccta ggggccgttc tggggaaaga gtgctttcag tcagctaaag atgactaata
     2761 tgtgaacata tattactaca catgccaatt tgtacattct ggatagttac cagccctggg
     2821 aaaacatcga aaaaaataaa tcaacataga taaaattggc aaatcccttc tggtacgatg
     2881 cagagtgctt gctctgagaa cttgtgcatt gtgacagaaa gcgaccgtta aaaactgaaa
     2941 gtagcatcta caacctgcat acatctgttc tttgaaaatc ccctgtaggc caaattaagc
     3001 atcacgctgt gctccacagc aacacggatg aaccgaaaac ccagacacaa acgcacacac
     3061 ttttcacata aacaaggttt agttatgcta gctatgcttt ttcgtcctcc ctttgtcatc
     3121 agcggcgagt gacgtaacac agtttgaccc tggacagcgt aaaggggggg ttgggaatga
     3181 gtgaaaggac tgtgacatct gcagggccag gggctgatgg agagagctga agtgagggag
     3241 gtcagaggag gaggaagggt gggatattgt cctcctcagg ccctatccct ctcagacgca
     3301 ttgattttta acctctcagc gagaatgcca agcattacac ccctcaaatt atggttcctc
     3361 acccaacctg ttatattttc cacctgcaga taattaaccg gtgccaccat ggagacgact
     3421 gtgaggtatg aacaggggtc agagctcact aaaacttcga gctctccaac agcagatgag
     3481 cccacgataa agattgatga tggtcgtgat gagggtaatg aacaagacag ctgttccaat
     3541 accattagga gaaaaatttc cccgtttgtg atgtcatttg gattcagagt atttggagtt
     3601 gtgcttatca ttgtagacat catagtggtg attgtggatc tggccatcag tgagaagaaa
     3661 agaggcatta gagagattct tgaaggtgtt tccctggcta tagcactctt cttccttgtt
     3721 gatgttctca tgagagtgtt tgttgaaggc ttcaagaact atttccggtc caaactggtt
     3781 actttggatg cagtcatagt agtgggcact ctgctaatta atatgaccta ctccttctct
     3841 gaccttgctg cctttaacag cgagaacgtg tatattaccg cggataaaca gaaaaacggc
     3901 attaaagcga actttaccgt gcgccataac gtggaagatg gcagcgtgca gctggcggat
     3961 cattatcagc agaacacccc gattggcgat ggcccggtgc tgctgccgga taaccattat
     4021 ctgagcaccc agaccgtgct gagcaaagat ccgaacgaaa aacgcgatca catggtgctg
     4081 ctggaatttg tgaccgcagc gggcattaca cacggcatgg atgaactgta tggcggcacc
     4141 ggcggcagcg cgagccaggg cgaagaactg tttaccggcg tggtgccgat tctggtggaa
     4201 ctggatggcg atgtgaacgg ccataaattt agcgtgcgcg gcgaaggcga aggcgatgcg
     4261 accattggca aactgaccct gaaatttatt tgcaccaccg gcaaactacc ggtgccgtgg
     4321 ccgaccctgg tgaccacctt aacctatggc gtgcagtgct ttagccgcta tccggatcat
     4381 atgaaacgcc atgatttttt taaaagcgcg atgccggaag gctatgtgca ggaacgcacc
     4441 attagcttta aagatgatgg caaatataaa acccgcgcgg tggtgaaatt tgaaggcgat
     4501 accctggtga accgcattga actgaaaggc accgatttta aagaagatgg caacattctg
     4561 gggcataaac tggaatataa cacagatcag atgccgcaca tggttactct tcttcgagtt
     4621 ctgaaaattg ttatcttaat aagaatattt cgcctggctt cacagaagaa acaacttgaa
     4681 gtggtaacct aatgaaccgg ttggatcctg gccgtcgttt tactgtacca tcgatgatga
     4741 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     4801 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     4861 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     4921 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
     4981 tctagatcag atctgcgaag atacggccac gggtgctctt gatcctgtgg ctgattttgg
     5041 actgtgctgc tcgcagctgc tgatgaatca catacttcct ccattttctt ccactgattg
     5101 actgttataa tttccctaat ttccaggtca aggtgctgtg cattgtggta atagatgtga
     5161 catgacgtca cttccaaagg accaatgaac atgtctgacc aatttcatat aatgtgaaaa
     5221 cgattttcat aggcagaata aataacattt aaattaaact gggcatcagc gcaattcaat
     5281 tggtttggta atagcaaggg aaaatagaat gaagtgatct ccaaaaaata agtacttttt
     5341 gactgtaaat aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa atgtaattaa
     5401 gtaaaagtaa aagtattgat ttttaattgt actcaagtaa agtaaaaatc cccaaaaata
     5461 atacttaagt acagtaatca agtaaaatta ctcaagtact ttacacctct ggttcttgac
     5521 cccctacctt cagcaagccc agcagatcca ctagttctag agcggccgcc accgcggtgg
     5581 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     5641 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     5701 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     5761 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     5821 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     5881 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5941 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6001 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6061 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6121 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6181 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6241 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6301 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6361 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6421 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6481 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6541 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6601 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6661 gctcagtgga acgaaaactc acgttaaggc attttggtca tgagattatc aaaaaggatc
     6721 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     6781 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6841 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6901 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     6961 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7021 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7081 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7141 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7201 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7261 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7321 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7381 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7441 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7501 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7561 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7621 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7681 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7741 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     7801 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     7861 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     7921 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     7981 gaaaaaccgt ctatcagggc gatggcccac gctggctaag aactcatcag cctccccggt
     8041 ccatctaccc acgtaccaat gcaccaattg gccacaatga cggctactac atggtgccat
     8101 tccttcctct ttataggaat ggagactacc tcctgtccaa caatgctctt ggatacgagt
     8161 acgcctacct gttggaccca ggtcattgca caacaccaga aatgccctct gatctgcaaa
     8221 agacgtgaat atctgttcag acacccatat ccactctgtt ccacacaggt cagaggtttg
     8281 tccaggagtt cttgacagag gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac
     8341 aagtacttag ggaaaatttt actcaattaa aagtaaaagt atctggctag aatcttactt
     8401 gagtaaaagt aaaaaagtac tccattaaaa ttgtacttga gtattaagga agtaaaagta
     8461 aaagcaagaa agaaaactag agattcttgt ttaagcttcc cgggtgtcga cgaattctcg
     8521 agctctattc ctaaagacct tgggtgacca aaatcttatt ttaataaata aaactgttta
     8581 ttaaaacttt tttgtttcaa agaaccatat gtatagtgaa atttataaaa atatcaattt
     8641 ttaaaaagct ggtgtactca tttatgttat gaactctaaa accatatact gactgcaagt
     8701 gatgatgtat agagtgatgt ttacgagtaa acatatttag ttgtatacat cctactgagc
     8761 acattttgat gtatgaaata acattacaag ctttatccaa attaagccat tttaaaacac
     8821 tgccaattga aaatacaaat cctggaaaaa atcgtcttta gcgcagtcat ttgagccatc
     8881 ctaatccgtt acctcagacc ataataagaa gggataacac tagctgtagc aatggaacac
     8941 atctgtttca cacaatcata tctcctgcgc cggtgctaag cagattcagc gtgatcataa
     9001 catgctttcc actcataaat gtaaatttac aatttgcaca tgtaaaacag acacttttga
     9061 gatattggat aaaaaaacaa gagtatattg cttagtttca tccaccagtc atccccacag
     9121 cgtttggaag gccataaaaa gtgtctaaaa tcaatgatca ttgaaagagc acaagagaga
     9181 ctcttacgct gtaatgccac tggggacaaa agtgacagtc tcttaatggg ctcttctgga
     9241 ggggctcctg aacattaaaa attatcagcg aaattaccga aagagcttca agcaactggc
     9301 atgcttgatc ctctgcgtcg gggcggtgaa taggtgcttc agatgccctc ttacccacgg
     9361 gctggattca gctgccccgc taccagcgga gaccccctaa tgagcctctg caattaagtt
     9421 tattcatgtt aagtgtgaac ggggtgcgtg cggaactgtg ggcagctaac agacctgggt
     9481 tctttgtgcc acaagtgctg cctttattcg gctcacaaag cagaaaacaa cacccgcacc
     9541 tataatggcg ccctcggctg ggtctaagaa acgtggcgag ttgacagagc agagtgggcg
     9601 gggttaagac agactgacag cgggacccat ctccatcctc ttattaacgc ttaacgagtg
     9661 ccttcctcat gcaatattca tcgccactaa tatcatccaa gctctgagct gagctggcca
     9721 cttatgtaag gcaattatgt aaaatatcag acagggccca cactcagaat ctgactgggg
     9781 tagagacgcg ggacgagaac cgagagcaag aactgaaagt gaaagtgacc actaaaggga
     9841 ggagaggaca gaggggcagg atgtgtcaag attaccagag aacacttggc cagaaatgcg
     9901 caaccattgg agctctccgg attacccaaa ggttaacgag tttgaacgcc tctgcccact
     9961 cgcccatctc tgatggtttc ccaagaactc ctcaagcaaa atatatataa ttgtgtgtat
    10021 tatgcacaga cacgagaaaa tgctgttttt ctgatctgca ttacagcaca tttgcccgcc
    10081 aacgacaata ccacccactc ggtacctcgc tgactcctga tgcctgatac ctgcgcggtg
    10141 actgtctaca atctgcataa tcaagagaag ttgtgttgaa gacgagcgcc acacaaccgt
    10201 ttccacaagg tcacccaagg ccggtgcaga tgtaggtgag gtctccataa acagactgaa
    10261 ataaacacat cctccgctgg gaacaacaac cccctcacgc ctcatgcatt tccataagcc
    10321 tacatgcatc tcttccaact tatggagact cgcacctacc aacatccgca caacaaagat
    10381 atacagagcg cgctccctca ggtcaaggcc ctgtgggggt ctgtgcagaa ataggtcatt
    10441 tgtcacacat caagtcctgg ggcaggagat gcattataga tgagaccaaa cagcctgtct
    10501 cggtgagctc tacccactcc ctgagactag aaatggggga agggagcttg agataacaac
    10561 cgctgcaatc actgtgtcga tgtttaatat cagcaccaac cgggaacaat aaggagatgg
    10621 gtgcattcat gttcacatct taccagtcaa gtatcatcga accggcttga taaccacacc
    10681 tcgtgtaata gctgagcaga tagttgtcat tttaaagcgt tggcctttgt cgattatgta
    10741 atgcgcacat tcaacacatg gtaatataga aacggttatg tcgaggttgt tttgtccaga
    10801 gatgaccttc acacagttac agccgctctg catccacaca aatggaggac ttaatcgtgg
    10861 actgcattct tagaaatgat ctacaaagac aaataatgtg aaatcaagaa aggacaaaat
    10921 ttaagtaagg ggatgaggga gagagagaac gaggggcaag gagaaagcat ggctcctgtc
    10981 tttttctgca cccatctgtt cggagtgcag gtggagctct attcactcag ctctgcatgt
    11041 gtgtttgggg ggggcaggaa gaaagggagg gcaaaaggaa gagtggagag atggtggggg
    11101 ctggagggat ggggggttct cggtgatctc tcctgaaggg gataatggga gagcagcgct
    11161 ttgcaatggc tgccatgtag taccctccct gcacaattag ccaatcagca gcaagctctg
    11221 ccagccagaa ggacacataa aagaagaaca ttgcagcaga ggcacagaag gagcctgcga
    11281 ggagctggga aatacacaca caacagcaga accacaacac cctcccctgg acacacccta
    11341 ctggggatca ctgcttttct ttttttctga accatcgccc acgccacacg gagagaaatc
    11401 tctctctcat catcatcctg aagaaaaccc ccttatcctc attttcacac tgctgaggaa
    11461 aacctacaat cgcacgggct gagatttcct ggcgaagact gtcctttttc ctttttcttt
    11521 ttttttcttt tcctttggaa actgacattt gcatttctcc attccaagcc acggcgtaat
    11581 aatatctgca atccagcctg aagacctgca aatcgaagga cctagatcac tatcatcttt
    11641 gtacgtcaag gatcctggtt actgtacgta taacctttct ttcttttgct ctgaccaata
    11701 tgaaacacta aaatcgattc gagcagcctc tcagcatcaa ttacagtgcg tgaaaaacat
    11761 tcaaatagag gcagcaaata ttacatgtga aaatacaggc tggctaaatc caagctaatt
    11821 tagaaatgtg gtcaaaacgc atactggcac gtctaatcgc ggattcagta aacaagatta
    11881 acgattagcc cagtgtataa gtcatatgat accggcatgc gcgagagcat cgctacaccc
    11941 gagctggctt cattttcgga ggaaaatcaa aacattgctt tctcctgccg tgcgaaccat
    12001 tcgtcataaa ccgtaatacg caacatacat ttattactac atccgttaat tagcgataat
    12061 tagccgttat taacaaagag cgctgaggaa ttcctctcaa atagcggagg tgcggcggag
    12121 gcagaggggc gtaaaagggc acatgcgtgc ctggctcaaa aaaggatgct ccagactgaa
    12181 actcagagac aaaacacgat gcctcggagg ctgagagccc atcgagagga aaggcaaaga
    12241 aaagggtcga gtcactcaga gggggagggg agatattgtg catccattgg tttgaattga
    12301 agcaggcaga ataaaaatcc ttgcgattat gcttcgtgag agatcgcgag agaggaaaag
    12361 gctaaatgcc acgtatgcaa atggataaat gtcatctatt tcttcgcgag tagacatttt
    12421 gtggacaacg agagtgtaaa taagcgatat gaggggaata caaaggcctt gagggaaagc
    12481 gttttgggcg tgtcttgtcg aaaagaagac agcgatggca cgcgctcaga accaccatct
    12541 tcacgtagac aatccgcgtg aaaacaataa caaaactgct cattttaaca cgagcaggtg
    12601 tttgaaacca cccaaaaaag attgtctaaa atattaagat taattaatga cgtttaaatg
    12661 tccaggcctg atataaatga agcgtgtagt aagagtaatc gcctttgttt cttttcagag
    12721 atacgttttc agagattcag ggaattatat cttcaataaa ataatgtttg atttcattat
    12781 tttaaagctt tttaagaagt aaaaatacac attattcatt tacattttat tttatttaaa
    12841 ggcctatggt cttgaaaata ataagtggaa tatataggct atatattcgt ctaaggacag
    12901 ccggtgttat tttatagtga cctcagacaa aagccgagac aaaacgaggc gcttgccttt
    12961 cattttaatc cttaatagac ggtgtgatga atgaatgaat gaatgaataa atgaatgaat
    13021 gacgggccct tcggcatgtt gttccgcagt cctccgtgag cacgagtctc taccgcagtg
    13081 ccaagcacat aaaaccgttg agtcagataa ccttctccag gcgcgtatac acaattcaag
    13141 gacaagcaca tcacaactat acacaacaca acacaaccgc ctaaacgcac gagctcatca
    13201 atcgcaattt aaaggcatgt cagtcaaaga aaaaggcggc gttatttaaa accaatgttc
    13261 aacaatattt gtatgctgac tagcccgggc gcgcacgcta taagatcaca aggccttgtt
    13321 ttttcccttt attcaaaata tcattaatta ttacaggtca taaaacaaaa caatgcgaat
    13381 tacaatgatt taaagactat tgatttaaca ataatcagtt aattatagaa taatattata
    13441 atattttaca tacaaatgaa agatgacatt atttgatgac attgtaatta aaatatatgc
    13501 aatatatttt tacgttttta ttaattaggc tattactatt attataatta tcattatttt
    13561 tattattatt attattatta ttaaatagtc ttgcgaaata agagtgaagg cagttgcaat
    13621 gttcattgtt cccggagagt cccgcagcct ctggcctcaa cacaaagcgc tactgtttac
    13681 attaatattc atagtgctgc catggtctct gtagaggagg aacgggtggc tcttttgttc
    13741 gctagtgtca atacgtttat tcagcaaaaa gcagctcgga tttgtgaatg cccaacaagc
    13801 acacgcgcac tccaatcaaa ggacgagagg ataacgattt taa
//
