LOCUS       sequence-398009-        9118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses LOV-Turbo1-NIK3x in the mammalian nucleolus under dox 
            inducible promoter, lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Qin W, Cheah JS, Xu C, Messing J, Freibaum BD, Boeynaems S, Taylor 
            JP, Udeshi ND, Carr SA, Ting AY
  TITLE     Dynamic mapping of proteome trafficking within and between living 
            cells by TransitID.
  JOURNAL   Cell. 2023 Jun 23:S0092-8674(23)00596-2. doi: 
            10.1016/j.cell.2023.05.044.
  PUBMED    37385249
REFERENCE   2  (bases 1 to 9118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2023 
            Jun 23:S0092-8674(23)00596-2. doi: 10.1016/j.cell.2023.05.044."
COMMENT     Sequence Label: V5-LOV-Turbo1-NIK3x_pTRE-G418
FEATURES             Location/Qualifiers
     source          1..9118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    181..414
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             599..643
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    856..973
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1027..1341
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1035..1053
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1071..1089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1106..1124
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1142..1160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1178..1196
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1213..1231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1249..1267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1314..1338
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1358..1367
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1367..1408
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        2920..3430
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3349..3367
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(3440..4057,4058..4063,4064..4099,4100..4102,4103..4138,
                     4139..4141,4142..4177,4178..4183)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             4193..4249
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4253..5275
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4253..5275
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     LTR             5466..5699
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5771..5892
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5808..5827)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5862..5881
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5932..6067
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6067..6138
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6139..6207,6208..6999)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7170..7758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7659..7678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7912..7929
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8046..8067
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8082..8087,8088..8105,8106..8112)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8120..8136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8125..8147
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8144..8160
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8144..8160
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8179..8199
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8181..8199
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8227..8453
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8454..8634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8681..8806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
       61 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
      121 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
      181 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
      241 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
      301 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
      361 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      421 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      481 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
      541 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      601 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      661 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      721 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      781 atcgtttcag acccacctcc caaccccgag gggacaattc tcgacctcga gacaaatggc
      841 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
      901 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
      961 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc cgcgaatcga
     1021 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
     1081 tgatagagaa cgatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
     1141 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
     1201 atgtcgagtt tatccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     1261 atagagaacg tatgtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt
     1321 ttagtgaacc gtcagatcgc ctggagaatt ggctagcgcc accatgggca agcccatccc
     1381 caaccccctg ctgggcctgg acagcaccgg taccaaagac aatactgtgc ctctgaagct
     1441 gatcgctctc ctggctaatg gcgagttcca tagtggcgaa cagctgggag aaaccctggg
     1501 catgtccagg gccgctatca acaagcacat tcagactctg cgcgactggg gcgtggacgt
     1561 gttcaccgtg cccggaaagg gctactctct gcccgagcct atcccgctgc tgaacgctaa
     1621 acagattctg ggacagctgg agtttcgggc aaccacactg gaacggatcg agaaaagctt
     1681 cgtgattact gatccgagac tgcctgacaa cccaatcatt tttgtgagcg attccttcct
     1741 gcagctgaca gaatattctc gggaagagat cctggggcgc aattgccgtt ttctgcaggg
     1801 acccgagaca gaccgtgcca ctgttcggaa aatcagagat gctattgaca accagactga
     1861 agtgaccgtt cagctgatca attataccaa gagcggcaag aagttctgga acgtgttcca
     1921 cctgcagccg atgcgcgatt ataagggcga cgtccagtac ttcattggcg tgcagctgga
     1981 tggcaccgaa cgtcttcatg gcgccgctga gcgtgaggcg gtctgcctgg tcaaaaagac
     2041 agcctttcag attgctgtgg cagtcctgcc tgtggtcgac tccaccaatc agtacctgct
     2101 ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc agcaggcagg
     2161 gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt acctgagtat
     2221 gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca tcggaattgt
     2281 catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat ggcccaatga
     2341 cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg gaataacagg
     2401 cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc gcgtggagga
     2461 aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc tggacaggaa
     2521 tactctggcc gctacgctga tccgagagct gcgggcagcc ctggaactgt tcgagcagga
     2581 aggcctggct ccatatctgc cacggtggga gaagctggat aacttcatca atagacccgt
     2641 gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg ataaacaggg
     2701 agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag aaatcagtct
     2761 gcggtctgcc gaaaagggat ccagaatgag aaagaagcga aaaaagaagc ttcgaatcct
     2821 gcgcatgaga aagaagcgca agaagaagct ccgcatcctc aggatgcgca agaaacgcaa
     2881 gaagaagctg cgcatcctgt aatagggccg aattcatccg gggttggggt tgcgcctttt
     2941 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
     3001 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
     3061 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
     3121 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
     3181 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
     3241 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
     3301 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
     3361 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
     3421 ctctccccag caattcacca tgtctagact ggacaagagc aaagtcataa acggagctct
     3481 ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
     3541 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
     3601 cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc
     3661 atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg
     3721 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
     3781 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
     3841 gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc
     3901 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat
     3961 tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
     4021 atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga
     4081 ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc
     4141 tgctgacgct cttgacgatt ttgaccttga catgctcccc gggggaagcg gagctactaa
     4201 cttcagcctg ctgaagcagg ctggagacgt ggaggagaac cctggacctg gtaaaaagcc
     4261 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     4321 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     4381 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     4441 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
     4501 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     4561 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
     4621 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     4681 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     4741 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     4801 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     4861 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     4921 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     4981 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
     5041 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     5101 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
     5161 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
     5221 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aataataacc
     5281 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     5341 ggatctccct ttgggccgcc tccccgcctg tttcgcctcg gcgtccggac tagaggtacc
     5401 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     5461 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     5521 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5581 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5641 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5701 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     5761 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5821 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5881 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     5941 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6001 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     6061 ggaggccttt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6121 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6181 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6241 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6301 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6361 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6421 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6481 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6541 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6601 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6661 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6721 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6781 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6841 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6901 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6961 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7021 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7081 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7141 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7201 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7261 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7321 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7381 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7441 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7501 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7561 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7621 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7681 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7741 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7801 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7861 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7921 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7981 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     8041 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     8101 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     8161 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     8221 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     8281 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     8341 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     8401 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     8461 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8521 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8581 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     8641 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     8701 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     8761 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     8821 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     8881 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     8941 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     9001 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     9061 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaac
//
