LOCUS       sequence-398021-       11875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FUGW lentiviral vector with constitutively expressed destabilized 
            GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in 
            combination with Ecad-RFP, comprises the Z-cad dual sensor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11875)
  AUTHORS   Toneff MJ, Sreekumar A, Tinnirello A, Hollander PD, Habib S, Li S, 
            Ellis MJ, Xin L, Mani SA, Rosen JM
  TITLE     The Z-cad dual fluorescent sensor detects dynamic changes between 
            the epithelial and mesenchymal cellular states.
  JOURNAL   BMC Biol. 2016 Jun 17;14(1):47.
  PUBMED    27317311
REFERENCE   2  (bases 1 to 11875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2016-06-17"; volume: "14(1)"; pages: "47"
COMMENT     Sequence Label: FUGW-d2GFP-ZEB1 3'UTR
FEATURES             Location/Qualifiers
     source          1..11875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(46..375)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      60..195
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(114..133)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     360..380
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(389..817)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(499..520)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     711..730
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    863..1087
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1076..1093
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             1119..1299
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1804..1820
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1805..1821
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1824..2412
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1930..1941
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2339..2359
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     ncRNA           complement(2436..2477)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     CDS             complement(join(4262..4384,4385..4390,4391..5101,5102..5104,
                     5105..5107))
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(4433..4454)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4782..4801
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5041..5062
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(5146..6357)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(5290..5309)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    6422..6539
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6837..6881)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7066..7299
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7792..7917
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7964..8144
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8162..8360)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8187..8207)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8362..8741
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8913..8932
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9007..9111
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9112..9180,9181..9972)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9330..9349)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10143..10731
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10632..10651
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10885..10902
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11019..11040
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11055..11060,11061..11078,11079..11085)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11093..11109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11098..11120
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11117..11133
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11117..11133
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    11182..11303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11193..11212)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11247..11266
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(11433..11807)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(11826..11873)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 gtgctgatca gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag
       61 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc
      121 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
      181 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
      241 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
      301 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
      361 ccctaactga cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca
      421 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
      481 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
      541 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
      601 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
      661 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
      721 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
      781 acacccgccg cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag
      841 ctggttcttt ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg
      901 tcttcccaat cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac
      961 ctactcagac aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca
     1021 ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa
     1081 ggcacagtcg aggctgatca gcgggtttaa acgggccctg ctagagattt tccacactga
     1141 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     1201 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     1261 gagctcccag gctcagatct ggtctaacca gagagaccca gtacagtccg gatgcagctc
     1321 tcgggccatg tgatgaaatg ctaggcggct gtcaaacctc cactctaata cttctctctc
     1381 cgggtcatcc atcccatgca ggctcacagg gtgtaacaag cgggtgttct ctccttcatt
     1441 ggcttcttct accttctctt gctcaactgg tactagcttg tagcaccatc caaaggtcag
     1501 tggatatctg atccctggcc ctggtgtgta gttctgccaa tcagggaagt agccttgtgt
     1561 gtggtagatc cacagatcaa ggatatcttg tcttcgttgg gagtgaatta gcccttccag
     1621 tccccccttt tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct
     1681 taaaggtacc tgaggtgtga ctggaaaacc cacctcctcc tcctcttgtg cttctagcca
     1741 ggcacaatca gcattggtag ctgctgtatt gctacttgtg attgctccat gtttttctag
     1801 gtctcgaggt cgacggtatc gatgcgggga ggcggcccaa agggagatcc gactcgtctg
     1861 agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag
     1921 gtccgctgga ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt
     1981 ggcaacacag gcgagcagcc aaggaaagga cgatgatttc cccgacaaca ccacggaatt
     2041 gtcagtgccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc
     2101 cgccgtggca atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc
     2161 aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct
     2221 gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa
     2281 aatgaaagcc atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca
     2341 tagcgtaaaa ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc
     2401 cagaggttga ttatcgataa gcttgatatc gaattggcca tttgttccat gtgagtgcta
     2461 gtaacaggcc ttgtgtcctg ctagcctagt acagcagttc aggcttgttg atgcatttta
     2521 ttgtgagatg ggagtctggt aaaaaactag aaaatgacca tgatgtaaca aggaacttaa
     2581 aaatgaagag tcagatctgt aacttacata catgaataca ggaacaaatt ggcacaaaat
     2641 gttaaacatt gttcccaaca ctgtttatag aagttaatcc taatttggct tactaaggaa
     2701 atgagagttg tatggttagg ctgatttctt aataaaactg cagagcatca tgctattagt
     2761 cacacagtga atttcaaaat ttaagtttta tattaaaaac tgggtaaagg taaaatgact
     2821 tgattgtagt tgtaaaacta atacattccc atttaagttc tgttctacag tccaaggcaa
     2881 gtataaatgt taacataaca ctgttaaatc aaatctcaat gcaggagaac caattgcatc
     2941 tctactttaa atcttatcaa ctttccaaat tttcatacta aaatatatta ttgtattaat
     3001 acaaactaca gtattataca ctacactgtg taataaataa agaaatataa aaataagaca
     3061 cataaatata aaagttttct aaaactaaaa gtacatatgt cagtaagaag ggtattaata
     3121 ctgccaggtt tgaagacata cagtacaaaa atgttgcaca gatctataaa ctaaaagaaa
     3181 taaaataata ctgataggta aaaatcagct aatgttgtta ataaattggg tccataataa
     3241 ctaacatttg gaaacagtta tgagccaaat aacaatagca tgtccatgtc tgaaatgcaa
     3301 gtacatggat aaagcagatt agaaaatttc cctttcgttt ctgtagagaa attctgaaaa
     3361 tcaatcaaca taaaatcaat accgaggaat tgaaggatga aatgtcccag tgtttcagtt
     3421 tctctgacag agtcagtggt tttaagtttt atttgggaat tttgatacaa gagacaaatc
     3481 aacaaatgct agttattgta ggccacacat tggatgaagg cgggttagag ccttgaaaat
     3541 actgagaaat ggcacttaca gcacacaggt cttgcttaag gccaaaggag atacaaagct
     3601 tcatgtcata tccttcatat gttaccacat attcaaacac aatcacaaac actgattctg
     3661 atgattttgc tgtccttatc agattaacat tgttagtccc tgcaatcaga actcaatggc
     3721 aatcttttga actccatttt aaaccacgtg agtaagaaac ttcagttctt ctatcagctg
     3781 ctttcttaag gctaacaata cactttaaat gataatacgc tctactaaga catctaaata
     3841 ttagaaatta ctaccttcta gactgaattt ataaaaggta gttagcacgg gttggaacta
     3901 cattatactt ctctgtgaag ggaattttgt agtgcagaag ttctcacatt ttaaaaaaca
     3961 taataaaata ctgaagcgga tttagcatat gtttaccaat aaaaaattat aaaatttctc
     4021 ccaattgtac agcttaagaa taaaccaaca taagagtgat aatactgata agataaaaca
     4081 atgccagatg catatctaat taatcagctt cctttaacct cttgtattgt tgctctctga
     4141 gtcattaagg ttttctaatc tgctgcatgt cacaaacttg ttcctaactt tgaaaactgc
     4201 atgaaaaatt actaggtctg aggttcaggc acacccggat ttattttgac taggaattcg
     4261 cctacacatt gatcctagca gaagcacagg ctgcagggtg acggtccatc ccgctctcct
     4321 gggcacaaga catgggcagc gtgccatcat cctgctcctc cacctccggc gggaagccat
     4381 ggctaagctt cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     4441 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc
     4501 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     4561 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
     4621 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
     4681 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     4741 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     4801 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     4861 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
     4921 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     4981 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     5041 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     5101 tcaccatggt gggatcctct agagtcgacc tgcagcccaa gcttcgtcta acaaaaaagc
     5161 caaaaacggc cagaatttag cggacaattt actagtctaa cactgaaaat tacatattga
     5221 cccaaatgat tacatttcaa aaggtgccta aaaaacttca caaaacacac tcgccaaccc
     5281 cgagcgcata gttcaaaacc ggagcttcag ctacttaaga agataggtac ataaaaccga
     5341 ccaaagaaac tgacgcctca cttatccctc ccctcaccag aggtccggcg cctgtcgatt
     5401 caggagagcc taccctaggc ccgaaccctg cgtcctgcga cggagaaaag cctaccgcac
     5461 acctaccggc aggtggcccc accctgcatt ataagccaac agaacgggtg acgtcacgac
     5521 acgacgaggg cgcgcgctcc caaaggtacg ggtgcactgc ccaacggcac cgccataact
     5581 gccgcccccg caacagacga caaaccgagt tctccagtca gtgacaaact tcacgtcagg
     5641 gtccccagat ggtgccccag cccatctcac ccgaataaga gctttcccgc attagcgaag
     5701 gcctcaagac cttgggttct tgccgcccac catgcccccc accttgtttc aacgacctca
     5761 cagcccgcct cacaagcgtc ttccattcaa gactcgggaa cagccgccat tttgctgcgc
     5821 tccccccaac ccccagttca gggcaacctt gctcgcggac ccagactaca gcccttggcg
     5881 gtctctccac acgcttccgt cccaccgagc ggcccggcgg ccacgaaagc cccggccagc
     5941 ccagcagccc gctactcacc aagtgacgat cacagcgatc cacaaacaag aaccgcgacc
     6001 caaatcccgg ctgcgacgga actagctgtg ccacacccgg cgcgtcctta tataatcatc
     6061 ggcgttcacc gccccacgga gatccctccg cagaatcgcc gagaagggac tacttttcct
     6121 cgcctgttcc gctctctgga aagaaaacca gtgccctaga gtcacccaag tcccgtccta
     6181 aaatgtcctt ctgctgatac tggggttcta aggccgagtc ttatgagcag cgggccgctg
     6241 tcctgagcgt ccgggcggaa ggatcaggac gctcgctgcg cccttcgtct gacgtggcag
     6301 cgctcgccgt gaggaggggg gcgcccgcgg gaggcgccaa aacccggcgc ggaggccaga
     6361 tctggagccg acacgggtta attaaccaaa ctggatctct gctgtccctg taataaaccc
     6421 gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta
     6481 ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaat
     6541 tgtggatgaa tactgccatt tgtctgcaga attggcgcac gcagtgccga tccgttcact
     6601 aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg
     6661 tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
     6721 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
     6781 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
     6841 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
     6901 tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc
     6961 caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc
     7021 acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc
     7081 tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg
     7141 agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact
     7201 ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
     7261 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
     7321 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
     7381 actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg
     7441 aagatcagcg gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt
     7501 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc
     7561 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg
     7621 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg
     7681 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg
     7741 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc
     7801 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg
     7861 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc
     7921 ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac
     7981 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
     8041 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
     8101 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca ggcaaaacgc
     8161 gctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
     8221 gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac
     8281 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat
     8341 gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca
     8401 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
     8461 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
     8521 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
     8581 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
     8641 gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc
     8701 cgtaattgat tactattaat aactagtcaa taatcaatgt caacgcgtat atctggcccg
     8761 tacatcgcga agcagcgcaa aacgcctaac cctaagcaga ttcttcatgc aattgtcggt
     8821 caagccttgc cttgttgtag cttaaatttt gctcgcgcac tactcagcga cctccaacac
     8881 acaagcaggg agcagatact ggcttaacta tgcggcatca gagcagattg tactgagagt
     8941 gcaccatagg ggatcgggag atctcccgat ccgtcgacgt caggtggcac ttttcgggga
     9001 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     9061 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     9121 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     9181 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     9241 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     9301 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     9361 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     9421 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     9481 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     9541 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     9601 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     9661 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     9721 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     9781 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     9841 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     9901 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     9961 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    10021 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    10081 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    10141 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    10201 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    10261 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    10321 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10381 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10441 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10501 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10561 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    10621 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    10681 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    10741 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    10801 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    10861 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
    10921 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
    10981 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
    11041 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
    11101 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
    11161 gtcgacggta tacagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
    11221 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
    11281 ttataagctg caataaacaa gttggggtgg gcgaagaact ccagcatgag atccccgcgc
    11341 tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag
    11401 gcggcggtgg aatcgaaatc tcgtagcacg tgtcagtcct gctcctcggc cacgaagtgc
    11461 acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg ctcgccgatc
    11521 tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc cgaccactcg
    11581 gcgtacagct cgtccaggcc gcgcacccac acccaggcca gggtgttgtc cggcaccacc
    11641 tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc ggcgaagtcg
    11701 tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac cgctccggcg
    11761 acgtcgcgcg cggtgagcac cggaacggca ctggtcaact tggccatggt ttagttcctc
    11821 accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc aacac
//
