LOCUS       sequence-398023-       10602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10602)
  AUTHORS   Wei Q, Adelstein RS
  TITLE     Conditional expression of a truncated fragment of nonmuscle myosin 
            II-A alters cell shape but not cytokinesis in HeLa cells.
  JOURNAL   Mol Biol Cell. 2000 Oct . 11(10):3617-27.
  PUBMED    11029059
REFERENCE   2  (bases 1 to 10602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2000 Oct . 11(10):3617-27."
COMMENT     Sequence Label: CMV-GFP-NMHC II-A
FEATURES             Location/Qualifiers
     source          1..10602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6..26)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      191..326
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             375..1169
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             375..1169
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(429..448)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1039..1058
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1357..1376)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1401..1448
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1777..2365
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2266..2285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2540..2843
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2844..3047
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2998..3018
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3086..3095
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3092..3094,3095..3097,3098..3808)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3092..3094,3095..3097,3098..3808)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3137..3158)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3398..3417)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3745..3766
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    9869..9990
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9906..9925)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9960..9979
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9997..10452)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10134..10155)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10346..10365
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10479..10583
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(10585..10602,1..340)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        join(10585..10602,1..174)
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
ORIGIN
        1 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
       61 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
      121 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
      181 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
      241 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
      301 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
      361 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
      421 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
      481 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
      541 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
      601 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
      661 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
      721 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
      781 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
      841 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
      901 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
      961 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     1021 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     1081 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     1141 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     1201 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     1261 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     1321 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
     1381 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
     1441 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     1501 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     1561 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
     1621 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1681 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1741 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1801 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1861 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1921 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1981 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2041 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2101 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2161 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2221 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2281 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2341 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2401 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2461 ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata
     2521 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     2581 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2641 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2701 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2761 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2821 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2881 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2941 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     3001 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
     3061 gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac
     3121 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     3181 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     3241 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     3301 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     3361 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     3421 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     3481 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     3541 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     3601 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     3661 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     3721 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     3781 cactctcggc atggacgagc tgtacaagta ctcagatctc gagctcaagc ttatggcaca
     3841 gcaagctgcc gataagtatc tctatgtgga taaaaacttc atcaacaatc cgctggccca
     3901 ggccgactgg gctgccaaga agctggtatg ggtgccttcc gacaagagtg gctttgagcc
     3961 agccagcctc aaggaggagg tgggcgaaga ggccatcgtg gagctggtgg agaatgggaa
     4021 gaaggtgaag gtgaacaagg atgacatcca gaagatgaac ccgcccaagt tctccaaggt
     4081 ggaggacatg gcagagctca cgtgcctcaa cgaagcctcg gtgctgcaca acctcaagga
     4141 gcgttactac tcagggctca tctacaccta ttcaggcctg ttctgtgtgg tcatcaatcc
     4201 ttacaagaac ctgcccatct actctgaaga gattgtggaa atgtacaagg gcaagaagag
     4261 gcacgagatg ccccctcaca tctatgccat cacagacacc gcctacagga gtatgatgca
     4321 agaccgagaa gatcaatcca tcttgtgcac tggtgaatct ggagctggca agacggagaa
     4381 caccaagaag gtcatccagt atctggcgta cgtggcgtcc tcgcacaaga gcaagaagga
     4441 ccagggcgag ctggagcggc agctgctgca ggccaacccc atcctggagg ccttcgggaa
     4501 cgccaagacc gtgaagaatg acaactcctc ccgcttcggc aaattcattc gcatcaactt
     4561 tgatgtcaat ggctacattg ttggagccaa cattgagact tatcttttgg agaaatctcg
     4621 tgctatccgc caagccaagg aagaacggac cttccacatc ttctattatc tcctgtctgg
     4681 ggctggagag cacctgaaga ccgatctcct gttggagccg tacaacaaat accgcttcct
     4741 gtccaatgga cacgtcacca tccccgggca gcaggacaag gacatgttcc aggagaccat
     4801 ggaggccatg aggattatgg gcatcccaga agaggagcaa atgggcctgc tgcgggtcat
     4861 ctcaggggtt cttcagctcg gcaacatcgt cttcaagaag gagcggaaca ctgaccaggc
     4921 gtccatgccc gacaacacag ctgcccaaaa ggtgtcccat ctcttgggta tcaatgtgac
     4981 cgatttcacc agaggaatcc tcaccccgcg catcaaggtg ggacgggatt acgtccagaa
     5041 ggcgcagact aaagagcagg ctgactttgc catcgaggcc ttggccaagg cgacctatga
     5101 gcggatgttc cgctggctgg tgctgcgcat caacaaggct ctggacaaga ccaagaggca
     5161 gggcgcctcc ttcatcggga tcctggacat tgccggcttc gagatctttg atctgaactc
     5221 gtttgagcag ctgtgcatca attacaccaa tgagaagctg cagcagctct tcaaccacac
     5281 catgttcatc ctggagcagg aggagtacca gcgcgagggc atcgagtgga acttcatcga
     5341 ctttggcctc gacctgcagc cctgcatcga cctcattgag aagccagcag gccccccggg
     5401 cattctggcc ctgctggacg aggagtgctg gttccccaaa gccaccgaca agagcttcgt
     5461 ggagaaggtg atgcaggagc agggcaccca ccccaagttc cagaagccca agcagctgaa
     5521 ggacaaagct gatttctgca ttatccacta tgccggcaag gtggattaca aagctgacga
     5581 gtggctgatg aagaacatgg atcccctgaa tgacaacatc gccacactgc tccaccagtc
     5641 ctctgacaag tttgtctcgg agctgtggaa ggatgtggac cgcatcatcg gcctggacca
     5701 ggtggccggc atgtcggaga ccgcactgcc cggggccttc aagacgcgga agggcatgtt
     5761 ccgcactgtg gggcagcttt acaaggagca gctggccaag ctgatggcta cgctgaggaa
     5821 cacgaacccc aactttgtcc gctgcatcat ccccaaccac gagaagaagg ccggcaagct
     5881 ggacccgcat ctcgtgctgg accagctgcg ctgcaacggt gttctcgagg gcatccgtat
     5941 ctgccgccag ggcttcccca acagggtggt cttccaggag tttcggcaga gatatgagat
     6001 cctgactcca aactccattc ccaagggttt catggacggg aagcaggcgt gcgtgctcat
     6061 gataaaagcc ctggagctcg acagcaatct gtaccgcatt ggccagagca aagtcttctt
     6121 ccgtgccggt gtgctggccc acctggagga ggagcgagac ctgaagatca ccgacgtcat
     6181 catagggttc caggcctgct gcaggggcta cctggccagg aaagcatttg ccaagcggca
     6241 gcagcagctt accgccatga aggtcctcca gcggaactgc gctgcctacc tgaagctgcg
     6301 gaactggcag tggtggcggc tcttcaccaa ggtcaagccg ctgctgcagg tgagccggca
     6361 ggaggaggag atgatggcca aggaggagga gctggtgaag gtcagagaga agcagctggc
     6421 tgcggagaac aggctcacgg agatggagac gctgcagtct cagctcatgg cagagaaatt
     6481 gcagctgcag gagcagctcc aggcagaaac cgagctgtgt gccgaggctg aggagctccg
     6541 ggcccgcctg accgccaaga agcaggaatt agaagagatc tgccatgacc tagaggccag
     6601 ggtggaggag gaggaggagc gctgccagca cctgcaggcg gagaagaaga agatgcagca
     6661 gaacatccag gagcttgagg agcagctgga ggaggaggag agcgcccggc agaagctgca
     6721 gctggagaag gtgaccaccg aggcgaagct gaaaaagctg gaggaggagc agatcatcct
     6781 ggaggaccag aactgcaagc tggccaagga aaagaaactg ctggaagaca gaatagctga
     6841 gttcaccacc aacctcacag aagaggagga gaaatctaag agcctcgcca agctcaagaa
     6901 caagcatgag gcaatgatca ctgacttgga agagcgcctc cgcagggagg agaagcagcg
     6961 acaggagctg gagaagaccc gccggaagct ggagggagac tccacagacc tcagcgacca
     7021 gatcgccgag ctccaggccc agatcgcgga gctcaagatg cagctggcca agaaagagga
     7081 ggagctccag gccgccctgg ccagagtgga agaggaagct gcccagaaga acatggccct
     7141 caagaagatc cgggagctgg aatctcagat ctctgaactc caggaagacc tggagtctga
     7201 gcgtgcttcc aggaataaag ctgagaagca gaaacgggac cttggggaag agctagaggc
     7261 gctgaaaaca gagttggagg acacgctgga ttccacagct gcccagcagg agctcaggtc
     7321 aaaacgtgag caggaggtga acatcctgaa gaagaccctg gaggaggagg ccaagaccca
     7381 cgaggcccag atccaggaga tgaggcagaa gcactcacag gccgtggagg agctggcgga
     7441 gcagctggag cagacgaagc gggtgaaagc aaacctcgag aaggcaaagc agactctgga
     7501 gaacgagcgg ggggagctgg ccaacgaggt gaaggtgctg ctgcagggca aaggggactc
     7561 ggagcacaag cgcaagaaag tggaggcgca gctgcaggag ctgcaggtca agttcaacga
     7621 gggagagcgc gtgcgcacag agctggccga caaggtcacc aagctgcagg tggagctgga
     7681 caacgtgacc gggcttctca gccagtccga cagcaagtcc agcaagctca ccaaggactt
     7741 ctccgcgctg gagtcccagc tgcaggacac tcaggagctg ctgcaggagg agaaccggca
     7801 gaagctgagc ctgagcacca agctcaagca ggtggaggac gagaagaatt ccttccggga
     7861 gcagctggag gaggaggagg aggccaagca caacctggag aagcagatcg ccaccctcca
     7921 tgcccaggtg gccgacatga aaaagaagat ggaggacagt gtggggtgcc tggaaactgc
     7981 tgaggaggtg aagaggaagc tccagaagga cctggagggc ctgagccagc ggcacgagga
     8041 gaaggtggcc gcctacgaca agctggagaa gaccaagacg cggctgcagc aggagctgga
     8101 cgacctgctg gtggacctgg accaccagcg ccagagcgcg tgcaacctgg agaagaagca
     8161 gaagaagttt gaccagctcc tggcggagga gaagaccatc tctgccaagt atgcagagga
     8221 gcgcgaccgg gctgaggcgg aggcccgaga gaaggagacc aaggctctgt cgctggcccg
     8281 ggccctggag gaagccatgg agcagaaggc ggagctggag cggctcaaca agcagttccg
     8341 cacggagatg gaggacctta tgagctccaa ggatgatgtg ggcaagagtg tccacgagct
     8401 ggagaagtcc aagcgggccc tagagcagca ggtggaggag atgaagacgc agctggaaga
     8461 gctggaggac gagctgcagg ccaccgaaga tgccaagctg cggttggagg tcaacctgca
     8521 ggccatgaag gcccagttcg agcgggacct gcagggccgg gacgagcaga gcgaggagaa
     8581 gaagaagcag ctggtcagac aggtgcggga gatggaggca gagctggagg acgagaggaa
     8641 gcagcgctcg atggcagtgg ccgcccggaa gaagctggag atggacctga aggacctgga
     8701 ggcgcacatc gactcggcca acaagaaccg ggacgaagcc atcaaacagc tgcggaagct
     8761 gcaggcccag atgaaggact gcatgcgcga gctggatgac acccgcgcct ctcgtgagga
     8821 gatcctggcc caggccaaag agaacgagaa gaagctgaag agcatggagg ccgagatgat
     8881 ccagttgcag gaggaactgg cagccgcgga gcgtgccaag cgccaggccc agcaggagcg
     8941 ggatgagctg gctgacgaga tcgccaacag cagcggcaaa ggagccctgg cgttagagga
     9001 gaagcggcgt ctggaggccc gcatcgccca gctggaggag gagctggagg aggagcaggg
     9061 caacacggag ctgatcaacg accggctgaa gaaggccaac ctgcagatcg accagatcaa
     9121 caccgacctg aacctggagc gcagccacgc ccagaagaac gagaatgctc ggcagcagct
     9181 ggaacgccag aacaaggagc ttaaggtcaa gctgcaggag atggagggca ctgtcaagtc
     9241 caagtacaag gcctccatca ccgccctcga ggccaagatt gcacagctgg aggagcagct
     9301 ggacaacgag accaaggagc gccaggcagc ctgcaaacag gtgcgtcgga ccgagaagaa
     9361 gctgaaggat gtgctgctgc aggtggatga cgagcggagg aacgccgagc agtacaagga
     9421 ccaggccgac aaggcatcta cccgcctgaa gcagctcaag cggcagctgg aggaggccga
     9481 agaggaggcc cagcgggcca acgcctcccg ccggaaactg cagcgcgagc tggaggacgc
     9541 cactgagacg gccgatgcca tgaaccgcga agtcagctcc ctaaagaaca agctcaggcg
     9601 cggggacctg ccgtttgtcg tgccccgccg aatggcccgg aaaggcgccg gggatggctc
     9661 cgacgaagag gtagatggca aagcggatgg ggctgaggcc aaacctgccg aataagtcga
     9721 ctctaatcac tagtgcggcc gatccaccgg atctagataa ctgatcataa tcagccatac
     9781 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     9841 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     9901 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9961 tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt
    10021 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
    10081 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
    10141 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
    10201 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
    10261 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
    10321 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
    10381 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
    10441 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
    10501 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
    10561 aataatattg aaaaaggaag agtcctgagg cggaaagaac ca
//
