LOCUS       sequence-398027-        6891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pTatABC with an mNeonGreen fluorescent protein tag at the C-terminal
            end of TatB..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Sharma A, Chowdhury R, Musser SM
  TITLE     Oligomerization state of the functional bacterial twin-arginine 
            translocation (Tat) receptor complex.
  JOURNAL   Commun Biol. 2022 Sep 19;5(1):988. doi: 10.1038/s42003-022-03952-2.
  PUBMED    36123532
REFERENCE   2  (bases 1 to 6891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-19"; volume: "5(1):988. doi"; pages: " 
            10.1038/s42003-022-03952-2"
COMMENT     Sequence Label: pTatABmNeonC
FEATURES             Location/Qualifiers
     source          1..6891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(96..974)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQFGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1001..1285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1208..1227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     regulatory      2436..2445
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3749..3766)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3919..4005
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4097..4124
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4143..4234
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4235..4303,4304..5095)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4453..4472)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5137..5592
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5224..5243)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5434..5455
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      5703..6291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6192..6211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6445..6462
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     6628..6650
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6788..6807
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatcccgaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca
     1321 tggccgtgta acgtataatg cggctttgtt taatcatcat ctaccacaga ggaacatgta
     1381 tgggtggtat cagtatttgg cagttattga ttattgccgt catcgttgta ctgctttttg
     1441 gcaccaaaaa gctcggctcc atcggttccg atcttggtgc gtcgatcaaa ggctttaaaa
     1501 aagcaatgag cgatgatgaa ccaaagcagg ataaaaccag tcaggatgct gattttactg
     1561 cgaaaactat cgccgataag caggcggata cgaatcagga acaggctaaa acagaagacg
     1621 cgaagcgcca cgataaagag caggtgtaat ccgtgtttga tatcggtttt agcgaactgc
     1681 tattggtgtt catcatcggc ctcgtcgttc tggggccgca acgactgcct gtggcggtaa
     1741 aaacggtagc gggctggatt cgcgcgttgc gttcactggc gacaacggtg cagaacgaac
     1801 tgacccagga gttaaaactc caggagtttc aggacagtct gaaaaaggtt gaaaaggcga
     1861 gcctcactaa cctgacgccc gaactgaaag cgtcgatgga tgaactacgc caggccgcgg
     1921 agtcgatgaa gcgttcctac gttgcaaacg atcctgaaaa ggcgagcgat gaagcgcaca
     1981 ccatccataa cccggtggtg aaagataatg aagctgcgca tgagggcgta acgcctgccg
     2041 ctgcacaaac gcaggccagt tcgccggaac agaagccaga aaccacgcca gagccggtgg
     2101 taaaacctgc tgcggacgct gaaccgaaaa ccgctgcacc ttccccttcg tcgagtgata
     2161 aaccgtatac ccgggtaccg atggtgtcca agggcgaaga ggacaacatg gccagcctgc
     2221 ctgccaccca cgagctgcac atcttcggca gcatcaacgg cgtggacttc gacatggtgg
     2281 gacagggcac cggcaacccc aacgacggct acgaggaact gaacctgaag tccacaaagg
     2341 gcgacctgca gttcagcccc tggattctgg tgccccacat cggctacggc ttccaccagt
     2401 acctgcccta ccccgacggc atgagccctt tccaagccgc catggtggat ggcagcggct
     2461 accaggtgca ccggaccatg cagtttgagg acggcgccag cctgaccgtg aactacaggt
     2521 acacatacga gggcagccac atcaagggcg aggcccaagt gaagggcaca ggctttccag
     2581 ccgacggccc cgtgatgacc aatagcctga cagccgccga ctggtgcaga agcaagaaaa
     2641 cctaccccaa tgacaagacc atcatcagca ccttcaagtg gtcctacacc accggcaatg
     2701 gcaagcggta cagaagcacc gcccggacca cctacacctt cgccaaaccc atggccgcca
     2761 actacctgaa gaaccagcct atgtacgtgt tccgcaagac cgagctgaag cactccaaga
     2821 cagaactgaa cttcaaagag tggcagaaag ccttcaccga cgtgatgggc atggacgagc
     2881 tgtacaagtg aactagtcat gtctgtagaa gatactcaac cgcttatcac gcatctgatt
     2941 gagctgcgta agcgtctgct gaactgcatt atcgcggtga tcgtgatatt cctgtgtctg
     3001 gtctatttcg ccaatgacat ctatcacctg gtatccgcgc cattgatcaa gcagttgccg
     3061 caaggttcaa cgatgatcgc caccgacgtg gcctcgccgt tctttacgcc gatcaagctg
     3121 acctttatgg tgtcgctgat tctgtcagcg ccggtgattc tctatcaggt gtgggcattt
     3181 atcgccccag cgctgtataa gcatgaacgt cgcctggtgg tgccgctgct ggtttccagc
     3241 tctctgctgt tttatatcgg catggcattc gcctactttg tggtctttcc gctggcattt
     3301 ggcttccttg ccaataccgc gccggaaggg gtgcaggtat ccaccgacat cgccagctat
     3361 ttaagcttcg ttatggcgct gtttatggcg tttggtgtct cctttgaagt gccggtagca
     3421 attgtgctgc tgtgctggat ggggattacc tcgccagaag acttacgcaa aaaacgcccg
     3481 tatgtgctgg ttggtgcatt cgttgtcggg atgttgctga cgccgccgga tgtcttctcg
     3541 caaacgctgt tggcgatccc gatgtactgt ctgtttgaaa tcggtgtctt cttctcacgc
     3601 ttttacgttg gtaaagggcg aaatcgggaa gaggaaaacg acgctgaagc agaaagcgaa
     3661 aaaactgaag aataaattca accgcctcta gagtcgacct gcaggcatgc aagcttggct
     3721 gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg
     3781 gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc
     3841 cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag
     3901 tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     3961 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
     4021 ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc
     4081 aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact
     4141 cttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4201 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4261 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4321 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4381 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4441 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     4501 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4561 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4621 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4681 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4741 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     4801 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4861 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4921 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     4981 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5041 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5101 agaccaagtt tactcatata tactttagat tgatttacgc gccctgtagc ggcgcattaa
     5161 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5221 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     5281 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5341 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5401 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5461 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
     5521 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5581 cgtttacaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5641 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5701 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5761 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5821 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     5881 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5941 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6001 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6061 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6121 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6181 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6241 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6301 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6361 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6421 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     6481 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg gtcatggctg
     6541 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     6601 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     6661 catcaccgaa acgcgcgagg cagaaggaga tggcgcccaa cagtcccccg gccacggggc
     6721 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
     6781 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
     6841 cggccacgat gcgtccggcg tagaggatct gctcatgttt gacagcttat c
//
