LOCUS       sequence-398064-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of a fusion of Zika NS2B-NS3 proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     The SARS-Cov-2 main protease (Mpro) is responsible for viral 
            polyprotein processing and therefore a therapeutic target.
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ZIKV NS2B-NS3 protease (H115A- catalytically
            i...
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(37..60,61..96,97..120)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gcgagctgga gtcacccaca attcgagaaa
       61 ggtggtggct ctggtggcgg ttcaggcggc tccgcctggt cccatccaca gtttgaaaag
      121 ggttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgag cggtaagtct
      181 gtcgatatgt acattgaacg tgccggtgac atcacctggg agaaggatgc agaagtgacg
      241 ggtaactcgc caagacttga tgttgcactc gacgagtcgg gcgattttag cttagttgaa
      301 gatgacggtc cgcctatgcg agagggcggc ggcggtagcg gcggtggtgg tggtagcgga
      361 gcattatggg atgtgccggc acccaaggaa gtaaagaaag gggagacgac agacggtgta
      421 tacagagtta tgacgagaag actgttgggt agcacacaag ttggtgttgg cgtaatgcag
      481 gaaggagtct tccacaccat gtgggcggtc accaagggca gtgcgcttcg cagcggtgag
      541 ggacgtctgg atccgtactg gggagacgtg aagcaagacc tggtcagtta ttgtggtccg
      601 tggaagcttg acgcagcctg ggacggccat agcgaggtgc aactcctcgc cgtaccgccg
      661 ggagagcgcg cccgtaatat tcaaactctg cccggtatct ttaaaaccaa ggacggagat
      721 ataggcgcag tggctcttga ttatcccgca gggactagcg gtagtcctat tctggataag
      781 tgcggccgag tgattggctt atacggaaat ggagtggtta tcaagaatgg aagctatgtc
      841 tcagccataa cccaaggtcg tcgcgaagag gagacacccg tagaataatg a
//
