LOCUS       sequence-398066-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of a fusion of Dengue2 NS2B-NS3 proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     The SARS-Cov-2 main protease (Mpro) is responsible for viral 
            polyprotein processing and therefore a therapeutic target.
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Fusion of Dengue2 NS2B-NS3 proteins
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(37..60,61..96,97..120)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gcgagctgga gtcacccaca attcgagaaa
       61 ggtggtggct ctggtggcgg ttcaggcggc tccgcctggt cccatccaca gtttgaaaag
      121 ggttctggtg tagatctggg taccgagaac ctgtacttcc aatccatggc cgacctcgaa
      181 ttagaaagag ccgcagatgt caagtgggaa gatcaggccg agatctccgg ttcttcaccc
      241 atactcagca taactattag cgaggatgga tccatgtcaa tcaagaacga agaggaagag
      301 cagacactgg gcggaggagg aagcggcggt ggcggggcag gtgtattatg ggacgtccca
      361 agtccgcccc caatgggaaa agccgaactg gaagatggcg cctatcgcat aaagcaaaag
      421 ggaatcttag gctatagtca gataggggcg ggtgtgtata aggaaggtac atttcacacc
      481 atgtgggcag tgacccgcgg ggcggtactc atgcataagg gaaagcggat tgagccaagc
      541 tgggccgatg taaagaaaga tttgatttcc tatggcggtg gatggaagct cgaaggcgag
      601 tggaaagagg gcgaagaggt ccaagttctg gccctggagc ctggtaagaa cccgcgtgcg
      661 gtgcaaacca agccaggcct gttcaagaca aacgcgggaa ctataggtgc cgtgtcgctg
      721 gacttctccc ctggcacctc aggttccccg attatcgaca agaaggggaa agtcgttggc
      781 ttatacggaa acggtgttgt tacgcgttct ggtgcgtacg tatccgcaat tgcccaaacg
      841 gagaagagca tcgaggataa cccggagatc gaggacgaca ttttcagaaa ataatga
//
