LOCUS       sequence-398073-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of a fusion of Dengue2 NS2B-NS3 proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     The SARS-Cov-2 main protease (Mpro) is responsible for viral 
            polyprotein processing and therefore a therapeutic target.
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Fusion of Dengue2 NS2B-NS3
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(37..60,61..96,97..120)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             145..165
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gcgagctgga gtcacccaca attcgagaaa
       61 ggtggtggct ctggtggcgg ttcaggcggc tccgcctggt cccatccaca gtttgaaaag
      121 ggttctggtg tagatctggg taccgagaac ctgtacttcc aatccatggc tgatctggaa
      181 ttagaacgag ctgcggatgt acgctgggaa gagcaggccg agatctcggg ttctagcccc
      241 attctttcta taaccatctc cgaagacggt agcatgtcaa tcaaaaatga agaagaggaa
      301 cagacactgg gtggtggagg atcaggagga ggaggtgctg gggtgctctg ggatgttccc
      361 tcccctcctc cggtaggtaa agcagagctg gaagatggcg cttaccggat aaaacaaaaa
      421 gggattctgg gttactcgca gattggagca ggagtttaca aggagggtac tttccacacg
      481 atgtggcatg ttactcgtgg tgcggtcctt atgcataaag gtaaacgcat cgaaccgtca
      541 tgggcagatg tcaaaaaaga ccttattagt tatggtggag gatggaagct ggaaggagaa
      601 tggaaagaag gcgaagaggt tcaagtgctg gcactggaac ctggtaagaa tccgcgtgcg
      661 gtacagacta aacctggctt attcaaaaca aataccggga caataggttg cgtttccctc
      721 gatttctccc cagggacctc cggctcacca attgttgata aaaaaggtaa agtagttggt
      781 ctttatggaa atggggtagt cacccgaagt ggtgcatatg tctctgccat agccaacacc
      841 gaaaaatcaa ttgaggacaa tccggaaatt gaagacgata tattccgcaa ataatga
//
