LOCUS       sequence-398075-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expresison of Enterovirus D68 2A protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  TITLE     The SARS-Cov-2 main protease (Mpro) is responsible for viral 
            polyprotein processing and therefore a therapeutic target.
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EV-D68 2A protease; 6xHis-SUMO, low expression...
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcaccacc atcatcacca tggctcagga gatcaggaag cgaaaccttc gaccgaggac
       61 ctgggcgaca agaaagaagg ggagtatatt aagttaaagg tgatcggcca ggactcaagc
      121 gagatccact tcaaagtcaa aatgaccacg cacctcaaga agttgaagga gagttactgt
      181 caacggcaag gggttcctat gaactcgctt cgcttcctgt tcgagggcca gcgaattgcg
      241 gacaaccaca cacctaagga acttggtatg gaagaggaag acgtcattga ggtgtaccag
      301 gagcagacag gcatgggtcc gggctttggg ggcgtatttg ttggaagttt taaaattatt
      361 aattaccacc ttgcaacgat tgaggagcgt cagtctgcaa tttacgtcga ttggcagagc
      421 gacgttcttg ttacccctat agcagcacac gggagacacc agattgcccg gtgtaagtgt
      481 aacacgggag tttattactg tagacatcgc gaccgcagtt atccaatttg ttttgaggga
      541 cctggaatcc agtggattga acaaaatgaa tactatcctg cacgttatca aacaaatgtg
      601 ttattggccg cgggcccggc ggaggctgga gattgtggcg gtttactggt ttgtccccac
      661 ggcgttatag gcctgttaac ggcggggggt ggaggtatcg tggcgtttac agacatacgg
      721 aatctgctgt ggctggatac ggatgtcatg gaacagtaat ga
//
