LOCUS       sequence-398077-        1167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expresison of Enterovirus D68 2A protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1167)
  TITLE     The SARS-Cov-2 main protease (Mpro) is responsible for viral 
            polyprotein processing and therefore a therapeutic target.
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EV-D68 2A protease; 6xHis-GST-TEV, low express...
FEATURES             Location/Qualifiers
     source          1..1167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             28..681
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             697..717
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc accaccatca ctcatcgatg tctcccattt tgggttattg gaagataaag
       61 gggttagtac aaccgaccag attactgtta gagtaccttg aagagaagta tgaagagcat
      121 ttatatgagc gcgacgaagg cgataagtgg cgcaacaaga aatttgagtt aggcctcgag
      181 tttccgaacc ttccttatta tatcgacggg gacgtaaagt tgacgcaatc tatggcaata
      241 attcggtata tagcagacaa gcataacatg ttgggtggct gccccaaaga gcgtgccgag
      301 atctccatgt tagaaggagc cgtcctggac attcgctatg gtgtgtcgcg cattgcctac
      361 tcaaaagatt tcgagacttt aaaagttgac tttctgagca agctgccgga gatgcttaag
      421 atgttcgaag atcggctgtg tcacaagacg tacctcaacg gtgaccacgt tacccaccca
      481 gactttatgc tttacgatgc gctggacgtt gtgctttaca tggacccgat gtgtctggac
      541 gcgtttccga agttggtatg tttcaagaag cggattgagg ctattcccca aatagacaaa
      601 taccttaaga gtagtaagta catcgcgtgg ccacttcaag gttggcaagc taccttcggc
      661 ggcggcgacc atcccccaaa gtcgggcggc ggcagcgaga acctgtactt ccaatccatg
      721 ggtccgggct ttgggggcgt atttgttgga agttttaaaa ttattaatta ccaccttgca
      781 acgattgagg agcgtcagtc tgcaatttac gtcgattggc agagcgacgt tcttgttacc
      841 cctatagcag cacacgggag acaccagatt gcccggtgta agtgtaacac gggagtttat
      901 tactgtagac atcgcgaccg cagttatcca atttgttttg agggacctgg aatccagtgg
      961 attgaacaaa atgaatacta tcctgcacgt tatcaaacaa atgtgttatt ggccgcgggc
     1021 ccggcggagg ctggagattg tggcggttta ctggtttgtc cccacggcgt tataggcctg
     1081 ttaacggcgg ggggtggagg tatcgtggcg tttacagaca tacggaatct gctgtggctg
     1141 gatacggatg tcatggaaca gtaatga
//
